Russian Journal of Genetics

, Volume 42, Issue 11, pp 1324–1328 | Cite as

The use of hybridization in breeding of eukaryotic microorganisms

  • G. I. Naumov
  • E. S. Naumova
  • V. I. Kondratieva
Theoretical Papers and Reviews


The article deals with the genetic bases of breeding of eukaryotic microorganisms. Using the data on the Saccharomyces yeasts, application of different genetic approaches and methods to breeding is discussed, including interstrain, interlinear, and distant interspecific hybridization, as well as heterosis, polyploidy, cytoduction, and meiotic recombination.


Mating Type Wine Yeast Eukaryotic Microorganism Wine Yeast Strain Molecular Karyotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Spencer, J.F.T. and Spencer, D.M., Genetic Improvement of Industrial Yeasts, Annu. Rev. Microbiol., 1983, vol. 89, pp. 121–142.CrossRefGoogle Scholar
  2. 2.
    Stewart, G.G. and Russel, I., One Hundred Years of Yeast Research and Development in the Brewing Industry, J. Inst. Brew., 1986, vol. 92, pp. 537–558.Google Scholar
  3. 3.
    Trivedi, N.V., Jacobson, G.K., and Tesch, W., Baker’s Yeast, CRC Crit. Rev. Biotechnol., 1986, vol. 4, no. 1, pp. 75–109.CrossRefGoogle Scholar
  4. 4.
    Subden, R.E., Current Developments in Wine Yeasts, CRC Crit. Rev. Biotechnol., 1987, vol. 5, no. 1, pp. 49–65.Google Scholar
  5. 5.
    Johnston, J.R., Brewing and Distilling Yeasts, Yeast Technology, Spencer, J.F.T. and Spencer, D.M., Eds., Berlin: Springer, 1990, pp. 89–104.Google Scholar
  6. 6.
    Inge-Vechtomov, S.G., Some Problems of Yeast Hybridization, Mikrobiol. Promyshl., 1971, no. 7, pp. 9–14.Google Scholar
  7. 7.
    Kosikov, K.M., Geneticheskie metody selektsii drozhzhei (gibridizatsiya, poliploidiya) (Genetic Methods of Yeast Breeding (Hybridization, Polyploidy)), Moscow: Nauka, 1979.Google Scholar
  8. 8.
    Naumov, G.I., Kondrat’eva, V.I., Naumova, T.I., and Gudkova, N.K., Genetic Basis of the Saccharomyces cerevisiae Yeast Classification: Study of Hybrid Ascospore Survival, Zh. Obshch. Biol., 1983, vol. 44, no. 5, pp. 648–660.PubMedGoogle Scholar
  9. 9.
    Inge-Vechtomov, S.G., Novel Genetic Strains of Saccharomyces cerevisiae, Vestn. Leningr. Univ., 1963, no. 21, pp. 117–125.Google Scholar
  10. 10.
    Zakharov, I.A., Kozhin, S.A., Kozhina, T.N., and Fedorova, I.V., Sbornik metodik po genetike drozhzheisakharomitsetov (Laboratory Manual on the Genetics of Saccharomycete Yeasts), Leningrad: Nauka, 1984.Google Scholar
  11. 11.
    Naumov, G.I., Naumova, E.S., and Querol, A., Genetic Study of Natural Introgression Supports Delimitation of Biological Species in the Saccharomyces sensu stricto Complex, Syst. Appl. Microbiol., 1997, vol. 20, pp. 595–601.Google Scholar
  12. 12.
    Naumov, G.I., Kondrat’eva, V.I., and Naumova, E.S., The Methods of Hybridization of Homothallic Yeast Haploids and Diploids, Biotekhnologia, 1986, no. 6, pp. 33–36.Google Scholar
  13. 13.
    Rose, M.D., Winston, F., and Hieter, Ph., Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1990, pp. 11–18.Google Scholar
  14. 14.
    Alikhanian, S.I. and Nalbandyan, G.M., Selection of Wine Yeasts with the Use of Mutagens: I. Generation of the Saccharomyces vini Strains for the Production of Natural, Strong Table Wines from the Vine Varieties with High Sugar Content, Genetika (Moscow), 1971, vol. 7, no. 9, pp. 125–132.Google Scholar
  15. 15.
    Alikhanian, S.I., Nalbandyan, G.M., and Avakyan, B.P., Selection of Wine Yeasts with the Use of Mutagens: II. Generation of Ethanol-Resistant Strains of Saccharomyces oviformis for the Production of Sherry Wines, Genetika (Moscow), 1971, vol. 7, no. 10, pp. 51–58.Google Scholar
  16. 16.
    Alikhanian, S.I., Nalbandyan, G.M., and Avakyan, B.P., Selection of Wine Yeasts with the Use of Mutagens: III. Generation of the Saccharomyces vini Strains for the Production of Champaign Wines, Genetika (Moscow), 1971, vol. 7, no. 11, pp. 99–106.Google Scholar
  17. 17.
    Naumova, E.S., Naumov, G.I., and Korkhola, M., Molecular Karyotypes of Different Genetic Lines of the Saccharomyces cerevisiae Yeast, Biotekhnologia, 1993, no. 4, pp. 2–5.Google Scholar
  18. 18.
    Marullo, P., Bely, M., Masneuf-Pomarède, I., et al., Inheritable Nature of Enological Quantitative Traits Is Demonstrated by Meiotic Segregation of Industrial Wine Yeast Strains, FEMS Yeast Res., 2004, vol. 4, no. 7, pp. 711–719.PubMedCrossRefGoogle Scholar
  19. 19.
    Naumov, G.I., Genetic Identification of Biological Species in the Saccharomyces sensu stricto Complex, J. Ind. Microbiol., 1996, vol. 17, pp. 295–302.CrossRefGoogle Scholar
  20. 20.
    Naumov, G.I., James, S.A., Naumova, E.S., et al., Three New Species in the Saccharomyces sensu stricto Complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1931–1942.PubMedGoogle Scholar
  21. 21.
    Kurtzman, C.P., Phylogenetic Circumscription of Saccharomyces, Kluyveromyces and Other Members of the New Genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma, and Zygotorulaspora, FEMS Yeast Res., 2003, vol. 4, pp. 233–245.PubMedCrossRefGoogle Scholar
  22. 22.
    Nilson-Tillgren, T., Kielland-Brandt, M.C., Holmberg, S., et al., Is Lager Yeast a Species Hybrid? Utilization of Intrinsic Genetic Variation in Breeding, Proc. 4th Symp. on Genetics of Industrial Microorganisms, Ikeda, Y. and Beppu, T., Eds., Tokyo: Kodanska, 1983, pp. 143–147.Google Scholar
  23. 23.
    Vaughan Martini, A. and Martini, A., Three Newly Delimited Species of Saccharomyces sensu stricto, Antonie van Leeuwenhoek, 1987, vol. 53, no. 2, pp. 77–84.CrossRefGoogle Scholar
  24. 24.
    Naumov, G.I., Naumova, E.S., Masneuf, I., et al., Natural Polyploidization of Some Cultured Yeast Saccharomyces sensu stricto: Auto and Allotetraploidy, Syst. Appl. Microbiol., 2000, vol. 23, pp. 442–449.PubMedGoogle Scholar
  25. 25.
    Naumova, E.S., Naumov, G.I., Masneuf-Pomarède, I., et al., Molecular Genetic Study of Introgression between Saccharomyces bayanus and S. cerevisiae, Yeast, 2000, vol. 22, pp. 1099–1115.CrossRefGoogle Scholar
  26. 26.
    Vaughan Martini, A., Saccharomyces paradoxus comb. nov., a Newly Separated Species of the Saccharomyces sensu stricto Complex, Syst. Appl. Microbiol., 1989, vol. 12, pp. 179–182.Google Scholar
  27. 27.
    Naumova, E.S., Chernookova, T.V., Skorikova, T.K., et al., Selection of Champagne Yeasts on the Basis of Interspecies Hybridization Saccharomyces cerevisiae × S. bayanus, Biotekhnologia, 1993, no. 7, pp. 8–13.Google Scholar
  28. 28.
    Masneuf, I., Murat, M.-L., Naumov, G.I., et al., Des hybrides Saccharomyces cerevisiae × S. bayanus var. uvarum presentant une forte aptitude à rèvèler des composès soufrès charactèristiques de l’arôme variètal des vins blancs de Sauvignon, J. Int. Sci. Vigne Vin., 2002, vol. 36, no. 4, pp. 205–212.Google Scholar
  29. 29.
    Gunge, N., Breeding of Bakers’ Yeast—Determination of the Ploidy and an Attempt to Improve Practical Properties, Japan. J. Genet., 1966, vol. 41, no. 3, pp. 203–214.Google Scholar
  30. 30.
    Sakai, K. and Takahashi, T., Estimation of Ploidies in Brewery Yeasts, Bull. Brew. Sci., 1972, vol. 18, pp. 29–36.Google Scholar
  31. 31.
    Takahashi, T., Genetic Analysis of German Wine Yeast, Bull. Brew. Sci., 1978, vol. 24, pp. 39–47.Google Scholar
  32. 32.
    Inge-Vechtomov, S.G., “Illegitimate” Copulation and Polyploidy in Yeasts, Genetika (Moscow), 1967, no. 11, pp. 100–109.Google Scholar
  33. 33.
    Roman, H. and Sands, S.M., Heterogeneity of Clones of Saccharomyces Derived from Haploid Ascospores, Proc. Natl. Acad. Sci. USA, 1953, vol. 39, pp. 171–179.PubMedCrossRefGoogle Scholar
  34. 34.
    Takagi, A., Harashima, S., and Oshima, Y., Construction and Characterization of Isogenic Series of Saccharomyces cerevisiae Polyploid Strains, Appl. Environ. Microbiol., 1983, vol. 45, no. 3, pp. 1034–1040.PubMedGoogle Scholar
  35. 35.
    Zakharov, I.A., Yurchenko, L.V., and Yarovoi, B.F., Cytoduction: An Autonomous Transfer of Cytoplasmic Hereditary Factors upon Yeast Cell Mating, Genetika (Moscow), 1969, vol. 5, no. 9, pp. 136–141.Google Scholar
  36. 36.
    Conde, J. and Fink, G.R., A Mutant of Saccharomyces cerevisiae Defective for Nuclear Fusion, Proc. Natl. Acad. Sci. USA, 1976, vol. 73, no. 10, pp. 3651–3655.PubMedCrossRefGoogle Scholar
  37. 37.
    Spodina, T.L. and Kozhina, T.N., Transmission of the Cytoplasmic Killing Determinant in the Yeast upon Cytoduction, Tsitol. Genet., 1977, vol. 11, no. 2, pp. 99–102.PubMedGoogle Scholar
  38. 38.
    Yurchenko, L.V., Heterokaryosis in the Saccharomyces cerevisiae Yeast, Genetika (Moscow), 1982, vol. 18, no. 9, pp. 1412–1422.Google Scholar
  39. 39.
    Dutcher, S.K., Internuclear Transfer of Genetic Information in kar-1/KAR1 Heterokaryons in Saccharomyces cerevisiae, Mol. Gen. Biol., 1981, vol. 1, no. 3, pp. 245–253.Google Scholar
  40. 40.
    Inge-Vechtomov, S.G. and Karpova, T.S., Cytoduction Selective System with the Use of Recessive Suppressors in the Saccharomyces Yeast, Genetika (Moscow), 1984, vol. 20, no. 3, pp. 398–407.Google Scholar
  41. 41.
    Naumov, G.I., Tyurina, L.V., Bur’yan, N.I., and Skorikova, T.K., Toxin Production and Composition of Industrial Populations of the Saccharomyces cerevisiae Yeast, Biotekhnologia, 1986, no. 4, pp. 26–34.Google Scholar
  42. 42.
    Ivannikova, Yu.V., Naumova, E.S., and Naumov, G.I., Detection of Viral dsRNA in the Saccharomyces bayanus Yeast, Dokl. Russ. Akad. Nauk, 2006, vol. 406, no. 5, pp. 709–711.Google Scholar
  43. 43.
    Lodder, J., Khoudokormoff, B., and Langejan, A., Melibiose-Fermenting Baker’s Yeast Hybrids, Antonie van Leeuwenhoek, 1969, vol. 35(suppl., part II), p. 9.Google Scholar
  44. 44.
    Tsai Chen-ko, Chang Bo-run, and Liu Hui-ping, Hybridization and Selection of Yeasts: I. Effect of Maltose Genes or Maltose Fermentation Ability in Saccharomyces, Acta Genet. Sinica, 1978, vol. 5, no. 1, pp. 9–18.Google Scholar
  45. 45.
    Thornton, R., The Introduction of Flocculation into a Homothallic Wine Yeast: A Practical Example of the Modification of Winemaking Properties by the Use of Genetic Techniques, Am. J. Enol. Vitic., 1985, vol. 36, no. 1, pp. 47–49.Google Scholar
  46. 46.
    Lahtchev, K.L., Pesheva, M., and Tzvetanov, O., Construction of Hybrid Yeasts with Increased Flocculation for White Wine Manufacture, J. Wine Res., 1991, vol. 2, no. 3, pp. 191–201.Google Scholar
  47. 47.
    Javelot, C., Girand, P., Colonna-Ceccaldi, B., and Vladescu, B., Introduction of Terpene-Producing Ability in a Wine Strain of Saccharomyces cerevisiae, J. Biotechnol., 1991, vol. 21, pp. 239–251.CrossRefGoogle Scholar
  48. 48.
    Miwa, H., Yamanaka, S., and Takinaki, K., Breeding of Saccharomyces Yeast for Improved Growth Rate on Acetate Medium, J. Ferment. Technol., 1978, vol. 56, no. 5, pp. 539–542.Google Scholar
  49. 49.
    Cai, J., Zhang, B., and Liu, Y., Hybridization and Selection of Yeasts: IV. Breeding of Yeasts for High Ethanol Production, Acta Microbiol. Sinica, 1982, vol. 22, no. 1, pp. 48–54.Google Scholar
  50. 50.
    Eschenbruch, R., Cresswell, K.J., Fisher, B.M., and Thornton, R.J., Selective Hybridization of Pure Culture Wine Yeasts: I. Elimination of Undesirable Wine-Making Properties, Eur. J. Appl. Microbiol. Biotechnol., 1982, vol. 14, pp. 155–158.CrossRefGoogle Scholar
  51. 51.
    Thornton, R.J., Selective Hybridization of Pure Culture Wine Yeasts: II. Improvement of Fermentation Efficiency and Inheritance of SO2 Tolerance, Eur. J. Appl. Microbiol. Biotechnol., 1982, vol. 14, pp. 159–164.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • G. I. Naumov
    • 1
  • E. S. Naumova
    • 1
  • V. I. Kondratieva
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia

Personalised recommendations