Russian Journal of Genetics

, Volume 42, Issue 2, pp 97–105 | Cite as

Generation and analysis of novel mutations of the Trithorax-like gene in Drosophila melanogaster

  • E. V. Fedorova
  • A. A. Ogienko
  • D. A. Karagodin
  • K. G. Aimanova
  • E. M. Baricheva
Molecular Genetics

Abstract

The Trithorax-like (Trl) gene of Drosophila melanogaster encodes the multifunctional GAGA factor. The expression of Trl is known to depend on numerous factors, such as the organ, the tissue, the ontogenetic stage, and the ambient temperature. Apparently, this expression is controlled by a complex system of regulatory elements, which so far has been scarcely studied. Our preliminary results indicate that the second intron of the Trl gene bears functionally significant elements. To test this assumption, we generated 23 novel alleles of the gene via P-induced male recombination and analyzed them cytogenetically. Of these mutations, 13 (recessive lethals) are deletions, disrupting the coding gene region. Ten mutations (seven deletions and three duplications) remove parts of the second Trl intron only. Some of these mutant stocks exhibit lower viability at different temperatures. These results suggest that the second intron region harbors functionally significant elements. The deletion mapping results verified the localization of the Trl gene in the 70F1-2 region.

Keywords

Ambient Temperature Complex System Gene Region Regulatory Element Numerous Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • E. V. Fedorova
    • 1
  • A. A. Ogienko
    • 1
  • D. A. Karagodin
    • 1
  • K. G. Aimanova
    • 1
  • E. M. Baricheva
    • 1
  1. 1.Institute of Cytology and GeneticsRussian Academy of SciencesNovosibirskRussia

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