Effect of Soil Drought on Biomass Production, Physiological Attributes and Antioxidant Enzymes Activities of Potato Cultivars
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The effects of water deficit on the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX), and also on total antioxidant capacity, ion leakage, proline content, chlorophyll fluorescence, gas exchange, chlorophyll and carotenoid content and plant dry mass of potato cultivars (Solanum tuberosum L.) were investigated. For this purpose, the response of ten potato cultivars (Agria, Arinda, Marfona, Banba, Born, Santé, Milva, Satina, Jelly and Spirit) was studied under two irrigation regimes (70 and 20% of soil available water). Water deficit increased the activity of antioxidant enzymes, ion leakage and proline content but decreased total antioxidant capacity, Fv/Fm, gas exchanges, chlorophyll, carotenoid and plant dry mass in all potato cultivars. Based on stress tolerance index (STI), Agria and Born were the most tolerant and sensitive cultivars to water stress, respectively. Positive correlations were found between total antioxidant capacity and the activities of CAT, APX and POX under control irrigation. Proline content positively correlated with plant dry mass and negatively with photosynthesis, transpiration and carbon dioxide concentration in the sub-stomatal chamber under water deficit treatment. The highest increases in ion leakage under water deficit were observed in the most sensitive cultivars. The results of this experiment, therefore, showed that proline accumulation and ion leakage may be related to water stress tolerance of potato cultivars. Also, increased chlorophyll and carotenoid content in resistant cultivars supported potato plants to tolerate water deficit.
Keywords:Solanum tuberosum antioxidant enzymes ion leakage proline
ACKNOWLEDGMENTS COMPLIANCE WITH ETHICAL STANDARDS
This work was supported by the Isfahan University of Technology, Faculty of Agriculture, and Department of Horticulture Science.
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 5.Afshari, M., Shekari, F., Azemkhani, R., Habibi, H., and Fotokian, M.H., Effects of foliar application of salicylic acid on growth and physiological attributes of cowpea under water stress conditions, Iran Agric. Res., 2013, vol. 32, pp. 56–70.Google Scholar
- 6.Lu, Y.Y., Deng, X.P., and Kwak, S.S., Overexpression of CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in transgenic sweet potato enhances tolerance and recovery from drought stress, Afr. J. Biotech., 2010, vol. 9, pp. 8378–8391.Google Scholar
- 8.Lin, K.H., Chao, P.Y., Yang, C.M., Cheng, W.C., Lo, H.F., and Chang, T.R., The effects of flooding and drought stresses on the antioxidant constituents in sweet potato leaves, Bot. Stud., 2006, vol. 47, pp. 417–426.Google Scholar
- 11.Bansal, K.C. and Nagarajan, S., Leaf water content, stomatal conductance and proline accumulation in leaves of potato (Solanum tuberosum L.) in response to water stress, Ind. J. Plant Physiol., 1986, vol. 29, pp. 397–404.Google Scholar
- 13.Nakano, Y. and Asada, K., Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.Google Scholar
- 21.Fernandez, G.C.J., Effective selection criteria for assessing stress tolerance, Proc. Int. Symp. on Adaptation of Vegetables and Other Food Crops in Temperature and Water Stress, Cuo, C.G., Ed., Taiwan, 1992, pp. 257–270.Google Scholar
- 22.Shock, C.C., Feibert, E.B.G., and Saunders, L.D., Potato yield and quality response to deficit irrigation, Hort. Sci., 1998, vol. 33, pp. 655–659.Google Scholar
- 30.Schafleitner, R., Gutierrez, R., Espino, R., Gaudin, A., Pérez, J., Martinez, M., Dominguez, A., Tincopa, L., Alvarado, C., Numberto, G., and Bonierbale, M., Field screening for variation of drought tolerance in (Solanum tuberosum L.) by agronomical, physiological and genetic analysis, Potato Res., 2007, vol. 50, pp. 71–85.CrossRefGoogle Scholar