Advertisement

The Response of Achyranthes bidentata Blume to Short-Term UV-B Exposure

  • J. LiEmail author
  • X. Han
  • C. Wang
  • L. Tang
  • W. Zhang
  • W. Qi
RESEARCH PAPERS
  • 24 Downloads

Abstract

Ultraviolet-B radiation (UV-B) can boost the accumulation of terpenoids in plants. Here, Ach-yranthes bidentata Blume plantlets were exposed to UV-B radiation for different durations (1, 2, 3 and 4 h) to understand its effect on the accumulation of the medicinally important secondary metabolites oleanolic acid and ecdysterone. Our results showed that UV-B radiation led to reduced chlorophyll a, chlorophyll b and carotenoid production. Additionally, enzymatic antioxidants accumulated in the plant to detoxify ROS under UV-B radiation. Nine known enzyme-encoding genes (HMGS, HMGR, PMK, FPS, SS, SE, GGPPS, β-AS and CAS) involved in secondary metabolism in A. bidentata were analyzed at the transcriptional levels post-UV-B treatment from 0 to 4 h. RT-qPCR analysis revealed an upregulation in the HMGS, HMGR, PMK, FPS, SS, SE, β-AS and CAS genes that are related to the production of oleanolic acid and ecdysterone. We observed a significant increase in oleanolic acid and ecdysterone content at 3 and 2 h UV-B radiation exposure, respectively. The present work provides evidence that UV-B radiation in mild doses can enhance the concentration of these secondary metabolites by upregulating the relative expression levels of key enzyme genes involved in oleanolic acid and ecdysterone biosynthesis. This study provides evidence that short-term UV-B treatments can increase A. bidentata secondary metabolite content and may be a safe approach for generating high oleanolic acid and ecdysterone producing plantlets.

Keywords:

Achyranthes bidentata UV-B radiation oleanolic acid ecdysterone gene expression RT-qPCR 

Notes

ACKNOWLEDGMENTS COMPLIANCE WITH ETHICAL STANDARDS

This work was supported by the National Nature Science Foundation of China [project no. 81274076] and the Key Projects of Henan Province Colleges and Universities [project no. 17A180026].

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

REFERENCES

  1. 1.
    Bornman, J.F., New trends in photobiology: target sites of UV-B radiation in photosynthesis of higher plants, J. Photochem. Photobiol. B: Biol., 1989, vol. 4, no. 2, pp. 145–158.CrossRefGoogle Scholar
  2. 2.
    Caldwell, M.M., Teramura, A.H., and Tevini, M., The changing solar ultraviolet climate and the ecological consequences for higher plants, Trends Ecol. Evol., 1989, vol. 4, no. 12, pp. 363–367.CrossRefGoogle Scholar
  3. 3.
    Heck, D.E., Vetrano, A.M., Mariano, T.M., and Laskin, J.D., UV-B light stimulates production of reactive oxygen species: unexpected role for catalase, J. Biol. Chem., 2003, vol. 278, no. 25, pp. 22432–22436.CrossRefGoogle Scholar
  4. 4.
    Chen, H.Z. and Han, R., He-Ne laser treatment improves the photosynthetic efficiency of wheat exposed to enhanced UV-B radiation, Laser Phys., 2014, vol. 24, pp. 1–7.Google Scholar
  5. 5.
    Tossi, V., Lombardo, C., Cassia, R., and Lamattina, L., Nitric oxide and flavonoids are systemically induced by UV-B in maize leaves, Plant Sci., 2012, vol. 193/194, pp. 103–109.CrossRefGoogle Scholar
  6. 6.
    Kumari, R., Agrawal, S.B., Singh, S., and Dubey, N.K., Supplemental ultraviolet-B induced changes in essential oil composition and total phenolics of Acorus calamus L. (sweet flag), Ecotoxicol. Environ. Saf., 2009, vol. 72, no. 7, pp. 2013–2019.CrossRefGoogle Scholar
  7. 7.
    Nishimura, T., Ohyama, K., Inagaki, N., Morota, T., and Goto, E., Ultraviolet-B radiation suppressed the growth and anthocyanin production of perilla plants grown under controlled environments with artificial light, Acta Hortic., 2008, vol. 797, pp. 425–430.Google Scholar
  8. 8.
    Indrajith, A. and Ravindran, K.C., Antioxidant potential of Indian medicinal plant Phyllanthus amarus L. under supplementary UV-B radiation, Recent Res. Sci. Technol., 2009, vol. 1, pp. 34–39.Google Scholar
  9. 9.
    Lee, M.J., Son, J.E., and Oh, M.M., Growth and phenolic content of sowthistle grown in a closed-type plant production system with a UV-A or UV-B lamp, Hortic. Environ. Biotechnol., 2013, vol. 54, no. 6, pp. 492–500.CrossRefGoogle Scholar
  10. 10.
    Li, Y., Liu, P., and Li, Y.H., Intraspecific variation of Achyranthes bidentata (Amaranthaceae) in the geo-authentic product area based on internal transcribed spacer sequences of ribosomal DNA, Aust. J. Crop Sci., 2012, vol. 6, pp. 1655–1660.Google Scholar
  11. 11.
    Li, J., Wang, C., Han, X., Qi, W., Chen, Y., Wang, T., Zheng, Y., and Zhao, X., Transcriptome analysis to identify the putative biosynthesis and transport genes associated with the medicinal components of Ac-hyranthes bidentata Bl., Front. Plant Sci., 2016, vol. 7: 1860.Google Scholar
  12. 12.
    Kumari, R. and Prasad, M.N.V., Medicinal plant active compounds produced by UV-B exposure, Sustainable Agric. Rev., 2013, vol. 12, pp. 225–254.CrossRefGoogle Scholar
  13. 13.
    Ramani, S. and Chelliah, J., UV-B-induced signalling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures, BMC Plant Biol., 2007, vol. 7, pp. 61–77.CrossRefGoogle Scholar
  14. 14.
    Pandey, N. and Pandey-Rai, S., Short term UV-B radiation-mediated transcriptional responses and altered secondary metabolism of in vitro propagated plantlets of Artemisia annua, Plant Cell Tissue Organ Cult., 2014, vol. 116, no. 3, pp. 371–385.CrossRefGoogle Scholar
  15. 15.
    Wellburn, A., The spectral determination of chlorophyll a and chlorophyll b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution, J. Plant Physiol., 1994, vol. 144, no. 3, pp. 307–313.CrossRefGoogle Scholar
  16. 16.
    Beauchamp, C. and Fridovich, I., Superoxide dismutase: improved assays and assay applicable to acrylamide gels, Anal. Biochem., 1971, vol. 44, no. 1, pp. 276–287.CrossRefGoogle Scholar
  17. 17.
    Dhindsa, R.S., Plumb-Dhindsa, P., and Thorpe, T.A., Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase, J. Exp. Bot., 1981, vol. 32, no. 1, pp. 93–101.CrossRefGoogle Scholar
  18. 18.
    Li, J.T. and Hu, Z.H., Accumulation and dynamic trends of triterpenoid saponin in vegetative organs of Achyranthus bidentata, J. Integr. Plant Biol., 2009, vol. 51, no. 2, pp. 122–129.CrossRefGoogle Scholar
  19. 19.
    Livak, K.J. and Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(–delta delta C(T)) method, Methods, 2001, vol. 25, pp. 402–408.CrossRefGoogle Scholar
  20. 20.
    Xiang, L., Zeng, L.X., Yuan, Y., Chen, M., Wang, F., Liu, X.Q., Zeng, L.J., Lan, Z., and Liao, Z.H., Enhancement of artemisinin biosynthesis by overexpressing dxr, cyp71av1 and cpr in the plants of Artemisia annua L., Plant Omics, 2012, vol. 5, pp. 503–507.Google Scholar
  21. 21.
    Zhou, L., Yang, G., Sun, H., Tang, J., Jian, Y., and Wang, Y., Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L., Front. Med., 2016, vol. 11, no. 1, pp. 1–10.Google Scholar
  22. 22.
    Zhang, J., Wang, J., and Tian, L.P., Advance in research on effect of enhanced UV-B radiation on plants, Chin. Agr. Sci. Bull., 2009, vol. 25, no. 22, pp. 104–108.CrossRefGoogle Scholar
  23. 23.
    Li, X.Y., Chen, H.Z., and Han, R., Effect of UV-B irradiation on seed germination and seedling growth of Arabidopsis, Chin. Bull. Bot., 2013, vol. 48, pp. 52–58.CrossRefGoogle Scholar
  24. 24.
    Swarna, K., Bhanumathi, G., and Murthy, S., Studies on the UV-B radiation induced oxidative damage in thylakoid photofunctions and analysis of the role of antioxidant enzymes in maize primary leaves, Bioscan, 2012, vol. 7, pp. 609–610.Google Scholar
  25. 25.
    Santos, I., Fidalgo, F., and Almeida, J.M., Biochemical and ultrastructural changes in leaves of potato plants grown under supplementary UV-B radiation, Plant Sci., 2004, vol. 167, no. 4, pp. 925–935.CrossRefGoogle Scholar
  26. 26.
    Wargent, J.J., Nelson, B.C., McGhie, T.K., and Barnes, P.W., Acclimation to UV-B radiation and visible light in Lactuca sativa involves up-regulation of photosynthetic performance and orchestration of metabolome-wide responses, Plant Cell Environ., 2015, vol. 38, pp. 929–940.CrossRefGoogle Scholar
  27. 27.
    Singh, A., Sarkar, A., Singh, S., Agrawal, S.B., and Tripathi, B., Investigation of supplemental ultraviolet-B-induced changes in antioxidative defense system and leaf proteome in radish (Raphanus sativus L. cv. truthful): an insight to plant response under high oxidative stress, Protoplasma, 2010, vol. 245, no. 1, pp. 75–83.CrossRefGoogle Scholar
  28. 28.
    Debeaujon, I., Peeters, A.J., Leon-Kloosterziel, K.M., and Koornneef, M., The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium, Plant Cell, 2001, vol. 13, pp. 853–871.CrossRefGoogle Scholar
  29. 29.
    Eichholz, I., Huyskens-Keil, S., Keller, A., Ulrich, D., Kroh, L.W., and Rohn, S., UV-B-induced chandes of volatile metabolites and phenolic compounds in blueberries (Vaccinium corymbusum L.), Food Chem., 2011, vol. 126, pp. 60–64.CrossRefGoogle Scholar
  30. 30.
    Park, M.J., Gwak, K.S., Yang, I., Kim, K.W., Jeung, E.B., Chang, J.W., and Choi, I.G., Effect of citral, eugenol, nerolidol and α-terpineol on the ultrastructural changes of Trichophyton mentagrophytes, Fitoterapia, 2009, vol. 80, pp. 290–296.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • J. Li
    • 1
    Email author
  • X. Han
    • 1
  • C. Wang
    • 1
  • L. Tang
    • 1
  • W. Zhang
    • 1
  • W. Qi
    • 1
  1. 1.College of Life Sciences, Henan Normal UniversityXinxiangChina

Personalised recommendations