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Sucrose Synthase (SuSy) Gene Expression: An Indicator for Cotton Fiber Initiation and Early Development

  • M. Ahmed
  • S. Akhtar
  • M. Fanglu
  • M. M. Hasan
  • A. A. Shahid
  • X. Yanang
  • M. B. Sarwar
  • A. Q. Rao
  • T. Husnain
  • X. Wang
RESEARCH PAPERS
  • 33 Downloads

Abstract

Cotton (Gossypium hirsutum L.) fiber initiation from ovule epidermal cells happen from 0 to 5 DPA, invertase (INV) and sucrose synthase (SuSy) are crucial for fiber initiation, cell expansion, and elongation. We used two commercial cotton varieties, Xuzhou 142-normal wild-type (WT) and Sea Island PimaS-4 (PimaS-4) as controls and compared with three fiber mutants, Xuzhou 142-fl (fl), Xuzhou 142-N (N) and Ligon lintless (Li). SuSy, INV activity, sugars and malate content were measured at fiber cell initiation and early development stage. Increased Susy activity was detected in WT, PimaS-4 and Li ovules at 0 DPA than the fiber mutant lines fl and N. On the other hand, fl mutant showed high sucrose contents than N and Li during 0 to 1 DPA. No significant changes happen in studied cotton lines with respect to INV from 1 to 5 DPA altogether. There was a significant difference in total soluble sugars and malate contents between WT and fl cotton ovules at early elongation stage (5 DPA). The results revealed that SuSy activity at anthesis day indicates the fate of ovule epidermal cells to bulge out and form fiber initials. The reduced SuSy activity in fl ovules at 0 DPA results in a lack of fiber cell initiation and lead to fiberless seed phenotype. The study will pave the way towards unraveling the mechanism of fiber initiation and development by exploring the role of different fiber-related genes.

Keywords:

Gossypium hirsutum sucrose synthase invertase cotton fiber development sugar metabolism day post anthesis 

Notes

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

REFERENCES

  1. 1.
    Basra, A.S. and Malik, C., Development of the cotton fiber, Int. Rev. Cytol., 1984, vol. 89, pp. 65–113.CrossRefGoogle Scholar
  2. 2.
    Kim, H.J. and Triplett, B.A., Cotton fiber growth in planta and in vitro. Models for plant cell elongation and cell wall biogenesis, Plant Physiol., 2001, vol. 127, pp. 1361–1366.CrossRefGoogle Scholar
  3. 3.
    Ahmed, M., Shahid, A.A., Din, S.U., Akhtar, S., Ahad, A., Rao, A.Q., Bajwa, K.S., Khan, M.A.U., Sarwar, M.B., and Husnain, T., An overview of genetic and hormonal control of cotton fiber development, Pak. J. Bot., 2018, vol. 50, pp. 433–443.Google Scholar
  4. 4.
    Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T., Pathway and control of sucrose import into initiating cotton fibre cells, Aust. J. Plant Biol., 2000, vol. 27, pp. 795–800.Google Scholar
  5. 5.
    Ruan, Y.L. and Chourey, P.S., A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds, Plant Physiol., 1998, vol. 118, pp. 399–406.CrossRefGoogle Scholar
  6. 6.
    Stiff, M.R. and Haigler, C.H., Recent advances in cotton fiber development. Flowering and fruiting in cotton, in Tennessee: The Cotton Foundation, 2012, pp. 163–192.Google Scholar
  7. 7.
    Raghavendra, K., Sheeba, J., and Santosh, H., Sucrose synthase, a major biomarker for sink strength in cotton, Cotton Res. J., 2013, vol. 5, pp. 158–171.Google Scholar
  8. 8.
    Xu, S.M., Brill, E., Llewellyn, D.J., Furbank, R.T., and Ruan, Y.L., Overexpression of a potato sucrose synthase gene in cotton accelerates leaf expansion, reduces seed abortion, and enhances fiber production, Mol. Plant., 2012, vol. 5, pp. 430–441.CrossRefGoogle Scholar
  9. 9.
    Shu, H., Zhou, Z., Xu, N., Wang, Y., and Zheng, M., Sucrose metabolism in cotton (Gossypium hirsutum L.) fibre under low temperature during fibre development, Eur. J. Agron., 2009, vol. 31, pp. 61–68.CrossRefGoogle Scholar
  10. 10.
    Nolte, K.D., Hendrix, D.L., Radin, J.W., and Koch, K.E., Sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules, Plant Physiol., 1995, vol. 109, pp. 185–1293.CrossRefGoogle Scholar
  11. 11.
    Fujii, S., Hayashi, T., and Mizuno, K., Sucrose synthase is an integral component of the cellulose synthesis machinery, Plant Cell Physiol., 2010, vol. 51, pp. 294–301.CrossRefGoogle Scholar
  12. 12.
    Zeng, Y.D., Sun, J.L., Bu, S.H., Deng, K.S., Tao, T., Zhang, Y.M., Zhang, T.Z., Du, X.M., and Zhou, B.L., EcoTILLING revealed SNPs in GhSus genes that are associated with fiber-and seed-related traits in upland cotton, Sci. Rep., 2016, vol. 6: 29250. doi 10.1038/srep29250CrossRefGoogle Scholar
  13. 13.
    Brill, E., van Thournout, M., White, R.G., Llewellyn, D., Campbell, P.M., Engelen, S., Ruan, Y.L., Arioli, T., and Furbank, R.T., A novel isoform of sucrose synthase is targeted to the cell wall during secondary cell wall synthesis in cotton fiber, Plant Physiol., 2011, vol. 157, pp. 40–54.CrossRefGoogle Scholar
  14. 14.
    Kleczkowski, L.A., Glucose activation and metabolism through UDP-glucose pyrophosphorylase in plants, Phytochemistry, 1994, vol. 37, pp. 1507–1515.CrossRefGoogle Scholar
  15. 15.
    Li, B., Yang, Y., Hu, W.R., Li, X.D., Cao, J.Q., and Fan, L., Over-expression of GhUGP1 in upland cotton improves fibre quality and reduces fibre sugar content, Plant Breed., 2015, vol. 134, pp. 197–202.CrossRefGoogle Scholar
  16. 16.
    Kleczkowski, L.A., Kunz, S., and Wilczynska, M., Mechanisms of UDP-glucose synthesis in plants, Crit. Rev. Plant Sci., 2010, vol. 29, pp. 191–203.CrossRefGoogle Scholar
  17. 17.
    Wang, L. and Ruan, Y.L., Regulation of cell division and expansion by sugar and auxin signaling, Front. Plant Sci., 2013, vol. 4: 163.Google Scholar
  18. 18.
    Wang, L. and Ruan, Y.L., Critical roles of vacuolar invertase in floral organ development and male and female fertilities are revealed through characterization of GhVIN1-RNAi cotton plants, Plant Physiol., 2016, vol. 171, pp. 405–423.CrossRefGoogle Scholar
  19. 19.
    Koch, K., Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development, Curr. Opin. Plant Biol., 2004, vol. 7, pp. 235–246.CrossRefGoogle Scholar
  20. 20.
    Weschke, W., Panitz, R., Gubatz, S., Wang, Q., Radchuk, R., Weber, H., and Wobus, U., The role of invertases and hexose transporters in controlling sugar ratios in maternal and filial tissues of barley caryopses during early development, Plant J., 2003, vol. 33, pp. 395–411.CrossRefGoogle Scholar
  21. 21.
    Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T., Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development, Plant Cell, 2003, vol. 15, pp. 952–964.CrossRefGoogle Scholar
  22. 22.
    Wang, L., Li, X.R., Lian, H., Ni, D.A., He, Y.K., Chen, X.Y., and Ruan, Y.L., Evidence that high activity of vacuolar invertase is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively, Plant Physiol., 2010, vol. 154, pp. 744–756.CrossRefGoogle Scholar
  23. 23.
    Abidi, N., Hequet, E., and Cabrales, L., Changes in sugar composition and cellulose content during the secondary cell wall biogenesis in cotton fibers, Cellulose, 2010, vol. 17, pp. 153–160.CrossRefGoogle Scholar
  24. 24.
    Wang, L., Cook, A., Patrick, J.W., Chen, X.Y., and Ruan, Y.L., Silencing the vacuolar invertase gene GhVIN1 blocks cotton fiber initiation from the ovule epidermis, probably by suppressing a cohort of regulatory genes via sugar signaling, Plant J., 2014, vol. 78, pp. 686–696.CrossRefGoogle Scholar
  25. 25.
    Wang, L. and Ruan, Y.L., New insights into roles of cell wall invertase in early seed development revealed by comprehensive spatial and temporal expression patterns of GhCWIN1 in cotton, Plant Physiol., 2012, vol. 160, pp. 777–787.CrossRefGoogle Scholar
  26. 26.
    Jin, Y., Ni, D.A., and Ruan, Y.L., Posttranslational elevation of cell wall invertase activity by silencing its inhibitor in tomato delays leaf senescence and increases seed weight and fruit hexose level, Plant Cell, 2009, vol. 21, pp. 2072–2089.CrossRefGoogle Scholar
  27. 27.
    Tang, X., Su, T., Han, M., Wei, L., Wang, W., Yu, Z., Xue, Y., Wei, H., Du, Y., and Greiner, S., Suppression of extracellular invertase inhibitor gene expression improves seed weight in soybean (Glycine max), J. Exp. Bot., 2016, vol. 68, pp. 469–482.Google Scholar
  28. 28.
    Ali, A., Iqbal, M., Ali, Q., Razzaq, A., and Nasir, I.A., Gene profiling for invertase activity: assessment of potato varieties for resistance towards cold induced sweetening, Adv. Life Sci., 2016, vol. 3, pp. 63–70.Google Scholar
  29. 29.
    Yang, J., Hu, W., Zhao, W., Chen, B., Wang, Y., Zhou, Z., and Meng, Y., Fruiting branch K+ level affects cotton fiber elongation through osmoregulation, Front. Plant Sci., 2016, vol. 7: 13.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • M. Ahmed
    • 1
    • 2
  • S. Akhtar
    • 2
  • M. Fanglu
    • 1
  • M. M. Hasan
    • 2
  • A. A. Shahid
    • 2
  • X. Yanang
    • 2
  • M. B. Sarwar
    • 2
  • A. Q. Rao
    • 2
  • T. Husnain
    • 2
  • X. Wang
    • 1
  1. 1.Institute of Crop Sciences, College of Agriculture and Biotechnology, Faculty of Agriculture Life and Environment Sciences Zhejiang UniversityHangzhouChina
  2. 2.National Centre of Excellence in Molecular Biology, Faculty of Life Sciences, University of the Punjab LahoreLahorePakistan

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