Sucrose Synthase (SuSy) Gene Expression: An Indicator for Cotton Fiber Initiation and Early Development
- 33 Downloads
Cotton (Gossypium hirsutum L.) fiber initiation from ovule epidermal cells happen from 0 to 5 DPA, invertase (INV) and sucrose synthase (SuSy) are crucial for fiber initiation, cell expansion, and elongation. We used two commercial cotton varieties, Xuzhou 142-normal wild-type (WT) and Sea Island PimaS-4 (PimaS-4) as controls and compared with three fiber mutants, Xuzhou 142-fl (fl), Xuzhou 142-N (N) and Ligon lintless (Li). SuSy, INV activity, sugars and malate content were measured at fiber cell initiation and early development stage. Increased Susy activity was detected in WT, PimaS-4 and Li ovules at 0 DPA than the fiber mutant lines fl and N. On the other hand, fl mutant showed high sucrose contents than N and Li during 0 to 1 DPA. No significant changes happen in studied cotton lines with respect to INV from 1 to 5 DPA altogether. There was a significant difference in total soluble sugars and malate contents between WT and fl cotton ovules at early elongation stage (5 DPA). The results revealed that SuSy activity at anthesis day indicates the fate of ovule epidermal cells to bulge out and form fiber initials. The reduced SuSy activity in fl ovules at 0 DPA results in a lack of fiber cell initiation and lead to fiberless seed phenotype. The study will pave the way towards unraveling the mechanism of fiber initiation and development by exploring the role of different fiber-related genes.
Keywords:Gossypium hirsutum sucrose synthase invertase cotton fiber development sugar metabolism day post anthesis
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 3.Ahmed, M., Shahid, A.A., Din, S.U., Akhtar, S., Ahad, A., Rao, A.Q., Bajwa, K.S., Khan, M.A.U., Sarwar, M.B., and Husnain, T., An overview of genetic and hormonal control of cotton fiber development, Pak. J. Bot., 2018, vol. 50, pp. 433–443.Google Scholar
- 4.Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T., Pathway and control of sucrose import into initiating cotton fibre cells, Aust. J. Plant Biol., 2000, vol. 27, pp. 795–800.Google Scholar
- 6.Stiff, M.R. and Haigler, C.H., Recent advances in cotton fiber development. Flowering and fruiting in cotton, in Tennessee: The Cotton Foundation, 2012, pp. 163–192.Google Scholar
- 7.Raghavendra, K., Sheeba, J., and Santosh, H., Sucrose synthase, a major biomarker for sink strength in cotton, Cotton Res. J., 2013, vol. 5, pp. 158–171.Google Scholar
- 13.Brill, E., van Thournout, M., White, R.G., Llewellyn, D., Campbell, P.M., Engelen, S., Ruan, Y.L., Arioli, T., and Furbank, R.T., A novel isoform of sucrose synthase is targeted to the cell wall during secondary cell wall synthesis in cotton fiber, Plant Physiol., 2011, vol. 157, pp. 40–54.CrossRefGoogle Scholar
- 17.Wang, L. and Ruan, Y.L., Regulation of cell division and expansion by sugar and auxin signaling, Front. Plant Sci., 2013, vol. 4: 163.Google Scholar
- 22.Wang, L., Li, X.R., Lian, H., Ni, D.A., He, Y.K., Chen, X.Y., and Ruan, Y.L., Evidence that high activity of vacuolar invertase is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively, Plant Physiol., 2010, vol. 154, pp. 744–756.CrossRefGoogle Scholar
- 27.Tang, X., Su, T., Han, M., Wei, L., Wang, W., Yu, Z., Xue, Y., Wei, H., Du, Y., and Greiner, S., Suppression of extracellular invertase inhibitor gene expression improves seed weight in soybean (Glycine max), J. Exp. Bot., 2016, vol. 68, pp. 469–482.Google Scholar
- 28.Ali, A., Iqbal, M., Ali, Q., Razzaq, A., and Nasir, I.A., Gene profiling for invertase activity: assessment of potato varieties for resistance towards cold induced sweetening, Adv. Life Sci., 2016, vol. 3, pp. 63–70.Google Scholar
- 29.Yang, J., Hu, W., Zhao, W., Chen, B., Wang, Y., Zhou, Z., and Meng, Y., Fruiting branch K+ level affects cotton fiber elongation through osmoregulation, Front. Plant Sci., 2016, vol. 7: 13.Google Scholar