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Russian Journal of Plant Physiology

, Volume 65, Issue 6, pp 842–848 | Cite as

The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat (Triticum aestivum) under Cold Stress

  • Y. Lv
  • C. H. Song
  • Q. W. Lu
  • Y. Tian
  • H. D. Li
  • D. Zhang
  • J. Yu
  • Q. H. Xu
  • J. Cang
Research Papers

Abstract

‘Dongnong Dongmai1 (Dn1)’ is a cultivar of wheat (Triticum aestivum L.) which may endure‒30°C in field with a reviving rate of 85%. ABA may enhance plant resistance. Aiming to reveal Dn1 coldresistant mechanism after exogenous ABA (exo-ABA) treated (control: non-ABA treated), some transcription factors (TFs) were selected and detected. Seedlings were prayed with exo-ABA at three-leaf stage, subsequently their tilling nodes were respectively sampled when it was at 5, 0,–10 and–25°C in field. Six TF genes (TabZIP1, TaWABI5, TaMYB1, TaMYB80, TaNAC2 and TaWRKY80) were selected and their expression at different low temperature was detected, the results showed that: in non-ABA treated tilling nodes, the expression of six TF genes all reached their peak level at–10°C; in exo-ABA treated tilling nodes, the expression of five TF genes (TabZIP1, TaWABI5, TaMYB80, TaNAC2 and TaWRKY80) reached their peak level at–25°C, and particularly the expression level of TabZIP1 was the highest in five TFs. In order to further defined the biological function of TabZIP1, the physical and chemical properties, protein secondary structure and the evolutionary tree was analyzed and predicted by some bioinformatics software or online program; TabZIP1 was localized in nuclear of tobacco (Nicotiana tabacum), which was observed by laser scanning confocal microscope (LSCM). This study initially exposed some TFs in Dn1 were upregulated to respond to the coldstress after exo-ABA treated, which indicated that a serious cold-responsive genes could be transcripted with the TFs assistance, and the results provided new theoretical basis to reveal the cold-reponsive mechanism of winter wheat.

Keywords

Triticum aestivum winter wheat exogenous ABA cold-stress transcription factors subcellular localization 

Abbreviations

ABA

abscisic acid

Dn1

winter wheat cv. Dongnong Dongmai1

exo-ABA

exogenous ABA

PEG

polyethylene glycol

TFs

transcription factors

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References

  1. 1.
    Preston, J.C. and Sandve, S.R., Adaptation to seasonality and the winter freez, Front. Plant Sci., 2013, vol. 4, pp. 1–18.Google Scholar
  2. 2.
    Kurepin, L.V., Dahal, K.P., and Savitch, L.V., Role of CBFs as integrators of chloroplast redox, phytochrome and plant hormone signaling during cold acclimation, Int. Mol. Sci., 2013, vol. 14, pp. 12 729–12 763.CrossRefGoogle Scholar
  3. 3.
    Wang, C., Gao, C., and Wang, L., Comprehensive transcriptional profiling of NaHCO3-stressed Tamarix hispida roots reveals networks of responsive genes, Plant Mol. Biol., 2014, vol. 84, pp. 145–157.CrossRefGoogle Scholar
  4. 4.
    Xu, Z.S., Chen, M., Li, L.C., and Ma, Y.Z., Functions and application of the AP2/ERF transcription factor family in crop improvement, J. Integr. Plant Biol., 2011, vol. 53, pp. 570–585.CrossRefPubMedGoogle Scholar
  5. 5.
    Landschulz, W.H., Johnson, P.F., and Knight, S.L., The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins, Science, 1988, vol. 240, pp. 1759–1764.CrossRefPubMedGoogle Scholar
  6. 6.
    Kobayashi, F., Maeta, E., Terashima, A., and Takumi, S., Positive role of a wheat HvABI5 ortholog in abiotic stress response of seedlings, Physiol. Plant., 2008, vol. 134, pp. 74–86.CrossRefPubMedGoogle Scholar
  7. 7.
    Mao, X.G., Zhang, H.Y., and Qian, X.Y., TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis, Exp. Bot., 2012, vol. 63, pp. 2933–2946.CrossRefGoogle Scholar
  8. 8.
    Lee, T.G., Jang, C.S., Kim, J.Y., Kim, D.S., Park, J.H., and Kim, D.Y., A MYB transcription factor (TaMyb1) from wheat roots is expressed during hypoxia: roles in response to the oxygen concentration in root environment and abiotic stresses, Physiol. Plant., 2007, vol. 129, pp. 375–385.CrossRefGoogle Scholar
  9. 9.
    Sreenivasulu, N., Harshavardhan, V.T., Govind, G., Seiler, C., and Kohli, A., Contrapuntal role of ABA: does it mediate stress tolerance or plant growth retardation under long-term drought stress? Gene, 2012, vol. 506, pp. 265–273.CrossRefPubMedGoogle Scholar
  10. 10.
    Liu, L.J., Cang, J., and Yu, J., Effects of exogenous abscisic acid on carbohydrate metabolism and the expression levels of correlative key enzymes in winter wheat under low temperature, Biosci. Biotechnol. Biochem., 2013, vol. 77, pp. 516–525.CrossRefPubMedGoogle Scholar
  11. 11.
    Cang, J., Yu, J., Liu, L.J., Yang, Y., Cui, H., Hao, Z.B., and Li, Z.F., Two-dimensional electrophoresis analysis of proteins in response to cold stress in extremely coldresistant winter wheat Dongnongdongmai 1 tillering nodes, J. Northeast Agric. Univ., 2012, vol. 19, pp. 27–35.Google Scholar
  12. 12.
    Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Arabidopsis Genome Initiative, Nature, 2000, vol. 408, no. 6814, pp. 796–815.CrossRefGoogle Scholar
  13. 13.
    The map-based sequence of the rice genome. International Rice Genome Sequencing Project, Nature, 2005, vol. 436, no. 7052, pp. 793–800.CrossRefGoogle Scholar
  14. 14.
    Schmutz, J. and Cannon, S.B., Genome sequence of the palaeopolyploid soybean, Nature, 2010, vol. 463, pp. 178–183.CrossRefPubMedGoogle Scholar
  15. 15.
    Shen, H., Cao, K., and Wang, X.A., Conserved proline residue in the leucine zipper region of AtbZIP34 and AtbZIP61 in Arabidopsis thaliana interferes with the formation of homodimer, Biochem. Biophys. Res. Commun., 2007, vol. 362, pp. 425–430.CrossRefPubMedGoogle Scholar
  16. 16.
    Yamamoto, M.P., Onodera, Y., Touno, S.M., and Takaiwa, F., Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Plant Physiol., 2006, vol. 141, pp. 1694–1707.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Gangappa, S.N., Crocco, C.D., Johansson, H., Datta, S., and Hettiarachchi, C., The Arabidopsis B-BOX protein BBX25 interacts with HY5, negatively regulating BBX22 expression to suppress seedling photomorphogenesis, Plant Cell, 2013, vol. 25, pp. 1243–1257.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Kang, S.G., Price, J., Lin, P.C., Hong, J.C., and Jang, J.C., The arabidopsis bZIP1 transcription factor is involved in sugar signaling, protein networking and DNA binding, Mol. Plant, 2010, vol. 3, pp. 361–373.CrossRefPubMedGoogle Scholar
  19. 19.
    Leeboo, J.A. and Park, C.J., In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter, Biochem. Biophys. Res. Commun., 2006, vol. 44, pp. 55–62.Google Scholar
  20. 20.
    Casaretto, J. and Ho, T.D., The transcription factors HvABI5 and HvVP1 are required for the ABA induction of gene expression in barley aleurone cells, Plant Cell, 2003, vol. 15, pp. 271–284.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Meng, X.B., Zhao, W.S., Lin, R.M., Wang, M., and Peng, Y.L., Identification of a novel rice bZIP-type transcription factor gene, OsbZIP1, involved in response to infection of Magnaporthe grisea, Plant Mol. Biol., 2005, vol. 23, pp. 301–313.CrossRefGoogle Scholar
  22. 22.
    Galka, M.M., Rajagopalan, N., Buhrow, L.M., and Nelson, K.M., Identification of interactions between abscisic acid and ribulose-1,5-bisphosphate carboxylase/oxygenase, PloS One, 2015, vol. 10, pp. 13–33.CrossRefGoogle Scholar
  23. 23.
    Liu, R., Xu, Y.H., and Jiang, S.C., Light-harvesting chlorophyll a/b-binding proteins, positively involved in abscisic acid signalling, require a transcription repressor, WRKY40, to balance their function, Exp. Bot., 2013, vol. 64, pp. 5443–5456.CrossRefGoogle Scholar
  24. 24.
    Wang, J.F., Cang, J., Yu, J., and Liu, L.J., Effects of exogenous abscisic acid on miRNA expression pattern of winter wheat Dongnongdongmai, J. Triticeae Crops, 2014, vol. 34, pp. 311–317.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • Y. Lv
    • 1
  • C. H. Song
    • 1
  • Q. W. Lu
    • 1
  • Y. Tian
    • 1
  • H. D. Li
    • 1
  • D. Zhang
    • 1
  • J. Yu
    • 1
  • Q. H. Xu
    • 1
  • J. Cang
    • 1
  1. 1.College of Life ScienceNortheast Agricultural University, Xiangfang DistrictHeilongjiang, HarbinChina

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