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Russian Journal of Plant Physiology

, Volume 65, Issue 6, pp 882–889 | Cite as

Exogenous Melatonin Protects Canola Plants from Toxicity of Excessive Copper

  • V. P. Kholodova
  • S. V. Vasil’ev
  • M. V. Efimova
  • P. Yu. Voronin
  • Z. F. Rakhmankulova
  • E. Yu. Danilova
  • Vl. V. Kuznetsov
Research Papers
  • 2 Downloads

Abstract

Physiological mechanisms of canola (Brassica napus L., cv. Westar) plant protection afforded by melatonin (at 0.1–100 μM) from copper salts (at 10–100 μM) were studied. Plants were cultivated on Hoagland–Snyder medium. At the age of 5 weeks, they were subjected to melatonin, copper sulfate, or their combination for 7 days. It was found that excessive copper in a nutrient medium inhibited the dry biomass accumulation against the control by 25–85%. Copper sulfate diminished the content of chlorophylls and carotenoids and functional activity of the thylakoid membranes in the chloroplasts. It increased 2.0–2.5 times the lipid peroxidation (LPO) intensity and the proline level up to 20 times. Melatonin reduced the changes caused by copper, and the degree of the protection depended on melatonin and CuSO4 concentrations. It was found that melatonin decreased the oxidative stress and proline accumulation, both induced by CuSO4. At first, we established the positive correlation (with the coefficient 0.8240) between the level of oxidative stress and proline content in the presence of CuSO4. Possible mechanisms of protection by melatonin and its biological role under conditions of technogenic stress are discussed.

Keywords

Brassica napus CO2/H2 gas exchange copper ions malondialdehyde melatonin photosynthetic pigments proline 

Abbreviations

LPO

lipid peroxidation

MDA

malondialdehyde

NPQ

nonphotochemical quenching

ROS

reactive oxygen species

PSII

photosystem II

ФPSII

photosynthetic quantum yield for PSII

Fv/Fm

maximal quantum yield

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. P. Kholodova
    • 1
  • S. V. Vasil’ev
    • 1
  • M. V. Efimova
    • 1
    • 2
  • P. Yu. Voronin
    • 1
  • Z. F. Rakhmankulova
    • 1
  • E. Yu. Danilova
    • 3
  • Vl. V. Kuznetsov
    • 1
    • 2
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Tomsk National Research State UniversityTomskRussia
  3. 3.Sechenov the First State Medical UniversityRussian Ministry of HealthMoscowRussia

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