Russian Journal of Plant Physiology

, Volume 65, Issue 6, pp 874–881 | Cite as

Relationship between Polyamines and Anaerobic Respiration of Wheat Seedling Root under Water-Logging Stress

  • H. Y. Du
  • D. X. Liu
  • G. T. Liu
  • H. P. LiuEmail author
  • R. Kurtenbach
Research Papers


To elucidate the relationship between polyamines and anaerobic respiration of wheat (Triticum aestivum L.) seedling root under water-logging stress, the contents of polyamines (PAs), lactate and alcohol, and the activities of anaerobic respiration enzymes were investigated in seedling roots of two wheat cultivars, Yumai no. 18 and Yangmai no. 9. On the 5th day after water-logging treatment, spermidine (Spd) and spermine (Spm) contents increased significantly, pyruvate decarboxylase (PDC) activity increased and there was no difference between two cultivars. Alcohol dehydrogenase (ADH) activity and alcohol content in Yangmai no. 9 increased more markedly than Yumai no. 18, while lactate dehydrogenase (LDH) activity and the lactate content in the Yumai no. 18 increased more markedly than Yangmai no. 9. Treatments with exogenous Spd and Spm resulted in enhancing the increases in ADH activity, alcohol content, and the levels of Spd and Spm. This concomitantly inhibited the increases in LDH activity and lactate content in Yumai no. 18 under water-logging stress, alleviating stress-induced injury to the seedlings. Treatment with exogenous inhibitor methylglyoxyl-bis-guanylhydrazone (MGBG), resulted in reducing the increases in ADH activity, alcohol content, and Spd and Spm levels, promoting the increases in LDH activity and lactate content in Yangmai no. 9 under water-logging stress, and aggravating the stress-induced injury to the seedlings. The results suggested that under water-logging stress, increased Spd and Spm could facilitate the tolerance of wheat seedling to the stress by enhancing the increases in ADH activity and alcohol content, and inhibiting the increases in LDH activity and lactate content.


Triticum aestivum wheat seedling polyamine anaerobic respiration enzymes water-logging stress 



alcohol dehydrogenase


lactate dehydrogenase




pyruvate decarboxylase


phenylmethanesulfonyl fluoride




relative dry weight increase rate


S-adenosylmethionine decarboxylase








N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid


thiamine pyrophosphate


trihydroxy methyl-aminomethane


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  1. 1.
    Christianson, J.A., Llewellyn, D.J., Dennis, E.S., and Wilson, I.W., Global gene expression responses to water-logging in roots and leaves of cotton (Gossypium hirsutum L.), Plant Cell Physiol., 2010, vol. 51, pp. 21–37.CrossRefPubMedGoogle Scholar
  2. 2.
    Jackson, M.B., Davies, W.J., and Else, M.A., Pressureflow relationships, xylem solutes and root hydraulic conductance in flooded tomato plants, Ann. Bot., 1996, vol. 77, pp. 17–24.CrossRefGoogle Scholar
  3. 3.
    Drew, M.C., Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, vol. 48, pp. 223–250.CrossRefPubMedGoogle Scholar
  4. 4.
    Mancuso, S. and Shabala, S., Water-logging signaling and tolerance in plants, in Programmed Cell Death and Aerenchyma Formation under Hypoxia, Fagerstedt, K.V., Ed., Berlin: Springer, 2010, pp. 99–118.Google Scholar
  5. 5.
    Kreuzwieser, J., Hauberg, J., Howell, K.A., Carroll, A., Rennenberg, H., Millar, A.H., and Whelan, J., Differential response of gray poplar leaves and roots underpins stress adaptation during hypoxia, Plant Physiol., 2009, vol. 149, pp. 461–473.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Almeida, A.M., Vriezen, W.H., and van der Straeten, D., Molecular and physiological mechanisms of flooding avoidance and tolerance in rice, Russ. J. Plant Physiol., 2003, vol. 50, pp. 743–751.CrossRefGoogle Scholar
  7. 7.
    Bansal, R. and Srivastava, J.P., Effect of water-logging on photosynthetic and biochemical parameters in pigeonpea, Russ. J. Plant Physiol., 2015, vol. 62, pp. 322–327.CrossRefGoogle Scholar
  8. 8.
    Settler, T.L. and Waters, I., Reviews of prospects for germplasm improvement for water-logging tolerance in wheat, barley and oats, Plant Soil, 2003, vol. 253, pp. 1–34.CrossRefGoogle Scholar
  9. 9.
    Goyal, M. and Asthir, B., Polyamine catabolism influences anti-oxidative defense mechanism in shoots and roots of five wheat genotypes under high temperature stress, Plant Growth Regul., 2010, vol. 60, pp. 13–25.CrossRefGoogle Scholar
  10. 10.
    Grzesiak, M., Filek, M., Barbasz, A., Kreczmer, B., and Hartikainen, H., Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses, Plant Growth Regul., 2013, vol. 69, pp. 177–189.CrossRefGoogle Scholar
  11. 11.
    Du, H., Zhou, X., Yang, Q., Liu, H., and Kurtenbach, R., Changes in H+-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress, Plant Growth Regul., 2015, vol. 75, pp. 1–10.CrossRefGoogle Scholar
  12. 12.
    Ricard, B., Couee, I., Raymond, P., Saglio, P.H., Saint-Ges, V., and Pradet, A., Plant metabolism under hypoxia and anoxia, Plant Physiol. Biochem., 1994, vol. 32, pp. 1–10.Google Scholar
  13. 13.
    Slocum, R.D., Polyamine biosynthesis in plant, in Polyamines in Plants, Slocum, R.D. and Flores, H., Eds., Florida: CRC, 1991, pp. 23–40.Google Scholar
  14. 14.
    Lee, T.M., Shieh, Y.J., and Chou, C.H., Role of putrescine in enhancing shoot elongation in Scirpus mucronatus under submergence, Physiol. Plant., 1996, vol. 96, pp. 419–424.CrossRefGoogle Scholar
  15. 15.
    Nada, K., Iwatani, E., Doi, T., and Tachibana, S., Effect of putrescine pretreatment to roots on growth and lactate metabolism in the root of tomato (Lycopersicon esculentum Mill.) under root-zone hypoxia, J. Jpn. Soc. Hort. Sci., 2004, vol. 73, pp. 337–339.CrossRefGoogle Scholar
  16. 16.
    Yiu, J.C., Liu, C.W., Fang, Y.T., and Lai, Y.S., Waterlogging tolerance of welsh onion (Allium fistulosum L.) enhanced by exogenous spermidine and spermine, Plant Physiol. Biochem., 2009, vol. 47, pp. 710–716.CrossRefPubMedGoogle Scholar
  17. 17.
    Mustroph, A. and Albrecht, G., Tolerance of crop plants to oxygen deficiency stress: fermentative activity and photosynthetic capacity of entire seedlings under hypoxia and anoxia, Physiol. Plant., 2003, vol. 117, pp. 508–520.CrossRefPubMedGoogle Scholar
  18. 18.
    Kato-Noguchi, H., Evaluation of the importance of lactate for the activation of ethanolic fermentation in lettuce roots in anoxia, Physiol. Plant., 2000, vol. 109, pp. 28–33.CrossRefGoogle Scholar
  19. 19.
    Kiriakos, K., Maria, D., Christakis, H., Kalliopi, A., and Roubelakis, A., A narrow-pore HPLC method for the identification and quantitation of free, conjugated, and bound polyamines, Anal. Biochem., 1993, vol. 214, pp. 484–489.CrossRefGoogle Scholar
  20. 20.
    Trought, M.C.T. and Drew, M.C., Effects of waterlogging on young wheat plants (Triticum aestivum L.) and on soil solutes at different temperatures, Plant Soil, 1982, vol. 69, pp. 311–326.CrossRefGoogle Scholar
  21. 21.
    Gibbs, J. and Greenway, H., Mechanisms of anoxia tolerance in plants. I. Growth, survival and anaerobic catabolism, Funct. Plant Biol., 2003, vol. 30, pp. 1–47.CrossRefGoogle Scholar
  22. 22.
    Armstrong, W., Brändle, R., and Jackson, M.B., Mechanisms of flood tolerance in plants, Acta Bot. Neerl., 1994, vol. 43, pp. 307–358.CrossRefGoogle Scholar
  23. 23.
    Davies, D.D., Anaerobic metabolism and the production of organic acids, in The Biochemistry of Plants, Davies, D.D., Ed., New York: Academic, 1980, pp. 581–611.Google Scholar
  24. 24.
    Roberts, J.K.M., Callis, J., Wemmer, D., Walbot, V., and Jardetzky, O., Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 3379–3383.CrossRefPubMedGoogle Scholar
  25. 25.
    Gupta, K., Dey, A., and Gupta, B., Plant polyamines in abiotic stress responses, Acta Physiol. Plant., 2013, vol. 35, pp. 2015–2036.CrossRefGoogle Scholar
  26. 26.
    Liu, Y. and Zhang, S., Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis, Plant Cell, 2004, vol. 16, pp. 3386–3399.CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Mitsuya, Y., Takahashi, Y., Berberich, T., Miyazaki, A., Matsumura, H., Takahashi, H., Terauchi, R., and Kusano, T., Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus, J. Plant Physiol., 2009, vol. 166, pp. 626–643.CrossRefPubMedGoogle Scholar
  28. 28.
    Dutra, N.T., Silveira, V., Azevedo, I.G., Gomes-Neto, L.R., Facanha, A.R., Steiner, N., Guerra, M.P., Floh, E.I.S., and Santa-Catarina, C., Polyamines affect the cellular growth and structure of pro-embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines, Physiol. Plant., 2013, vol. 148, pp. 121–132.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • H. Y. Du
    • 1
  • D. X. Liu
    • 2
  • G. T. Liu
    • 3
  • H. P. Liu
    • 1
    Email author
  • R. Kurtenbach
    • 1
  1. 1.College of Life Science and Agronomy, Key Laboratory of Plant Genetics and Molecular BreedingZhoukou Normal UniversityZhoukou HenanChina
  2. 2.Jiangsu Provincial Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhou JiangsuChina
  3. 3.College of Biological ScienceChina Agricultural UniversityBeijingChina

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