Russian Journal of Plant Physiology

, Volume 65, Issue 6, pp 824–832 | Cite as

Phosphoenolpyruvate Carboxylase during Maturation and Germination Sorghum Seeds: Enzyme Activity and Regulation

  • Y. Bouargalne
  • R. Ben Mrid
  • R. El Omari
  • M. NhiriEmail author
Research Papers


Sorghum (Sorghum bicolor (L.) Moench) is a species of great socio-economic and ecological importance for countries in arid and semi-arid climate. In C4 plants like sorghum, phosphoenolpyruvate carboxylase (PEPC, EC plays a key role in seed development and germination. In this work, the PEPC activity shows an increase followed by a decrease at the early and later stages of maturation, respectively. In germinating seeds, the PEPC activity quickly increases after soaking. The L-malate test and the ratio of PEPC activity determined at pH 8.0 and 7.1, indicates, that PEPC is phosphorylated at the early stages of maturation then becomes dephosphorylated at the later stages and during seed germination, PEPC takes back its phosphorylated form. The determination of the affinity constant showed different KM depending on the seed developmental stage. As there is no PEPC-C4 isoform in developing sorghum seeds, this result indicates that the different KM observed during seed maturation could be a result of a post-translational regulation such as phosphorylation or ubiquitination of a pre-existing isoform. This regulation enhances the PEPC activity at early stages of seed development.


Sorghum bicolor C4 plant seed development phosphoenolpyruvate carboxylase enzyme activity phosphorylation L-malate glucose-6-phosphate 



crassulacean acid metabolism


days post-anthesis


days post-imbibition


ethylenedinitrilotetraacetic acid


ethylene-bis(oxyethylenenitrilo) tetraacetic acid


4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid


malate dehydrogenase


4-morpholinepropanesulfonic acid




phosphoenolpyruvate carboxylase


PEPC protein kinase


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • Y. Bouargalne
    • 1
  • R. Ben Mrid
    • 1
  • R. El Omari
    • 1
  • M. Nhiri
    • 1
    Email author
  1. 1.Laboratoire de Biochimie et Génétique MoléculaireFaculté des Sciences et Techniques de TangerTangerMaroc

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