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Russian Journal of Plant Physiology

, Volume 65, Issue 6, pp 824–832 | Cite as

Phosphoenolpyruvate Carboxylase during Maturation and Germination Sorghum Seeds: Enzyme Activity and Regulation

  • Y. Bouargalne
  • R. Ben Mrid
  • R. El Omari
  • M. Nhiri
Research Papers
  • 5 Downloads

Abstract

Sorghum (Sorghum bicolor (L.) Moench) is a species of great socio-economic and ecological importance for countries in arid and semi-arid climate. In C4 plants like sorghum, phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) plays a key role in seed development and germination. In this work, the PEPC activity shows an increase followed by a decrease at the early and later stages of maturation, respectively. In germinating seeds, the PEPC activity quickly increases after soaking. The L-malate test and the ratio of PEPC activity determined at pH 8.0 and 7.1, indicates, that PEPC is phosphorylated at the early stages of maturation then becomes dephosphorylated at the later stages and during seed germination, PEPC takes back its phosphorylated form. The determination of the affinity constant showed different KM depending on the seed developmental stage. As there is no PEPC-C4 isoform in developing sorghum seeds, this result indicates that the different KM observed during seed maturation could be a result of a post-translational regulation such as phosphorylation or ubiquitination of a pre-existing isoform. This regulation enhances the PEPC activity at early stages of seed development.

Keywords

Sorghum bicolor C4 plant seed development phosphoenolpyruvate carboxylase enzyme activity phosphorylation L-malate glucose-6-phosphate 

Abbreviations

CAM

crassulacean acid metabolism

DPA

days post-anthesis

DPI

days post-imbibition

EDTA

ethylenedinitrilotetraacetic acid

EGTA

ethylene-bis(oxyethylenenitrilo) tetraacetic acid

HEPES

4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid

MDH

malate dehydrogenase

MOPS

4-morpholinepropanesulfonic acid

PEP

phosphoe-nolpyruvate

PEPC

phosphoenolpyruvate carboxylase

PEPC-K

PEPC protein kinase

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • Y. Bouargalne
    • 1
  • R. Ben Mrid
    • 1
  • R. El Omari
    • 1
  • M. Nhiri
    • 1
  1. 1.Laboratoire de Biochimie et Génétique MoléculaireFaculté des Sciences et Techniques de TangerTangerMaroc

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