Russian Journal of Plant Physiology

, Volume 65, Issue 5, pp 658–666 | Cite as

Seasonal Changes in Primary Photosynthetic Events during Low Temperature Adaptation of Pinus sylvestris in Central Yakutia

  • V. E. Sofronova
  • T. K. Antal
  • O. V. Dymova
  • T. K. Golovko
Research Papers


The methods of chlorophyll fluorescence induction and HPLC were used to study the influence of autumnal temperature decrease on photochemical electron-transport activity of photosystem II (PSII), nonphotochemical quenching of excessive excitation energy, and the composition of pigments in the firstyear needles of Pinus sуlvestris L. trees grown naturally in Central Yakutia. In the period from the beginning of September to October 10, the chlorophyll content was reduced by half, while the Chl a/b ratio increased from 2.9 to 4.3–4.5, indicating the degradation of peripheral antenna complexes. The decrease in average daily temperature to 4.9–6.4°C led to a transient increase in the quantum yield of nonphotochemical quenching (ΔpH-dependent parameter φNPQ). These changes were accompanied by a slow accumulation of unregulated zeaxanthin fraction insensitive to illumination conditions. The further decrease in average daily temperature to near-zero levels was paralleled by a sharp increase in zeaxanthin content, while the pH-dependent quenching was replaced with the constitutive quenching (parameter φf,D) because of the supposed structural reorganization of PSII. These processes were accompanied by a fast decrease in PSII functional activity, which was mostly due to the impairment of plastoquinone photochemical reduction. Freezing temperatures (from–3.6 to–12.1) destructed the oxygen-evolving complex in PSII and completely inactivated the PSII reaction centers. It is concluded that the largest changes in the condition of photosynthetic apparatus occur at a near-zero temperature range and proceed until complete inactivation of PSII under the action of freezing temperatures.


Pinus sylvestris needles chlorophyll fluorescence photosynthetic pigments seasonal dynamics low-temperature adaptation 









electron-transport chain


light-harvesting complex




nonphotochemical quenching of Chl fluorescence


oxygen-evolving complex


photosynthetically active radiation


photon flux density




photosystem I


photosystem II


photosynthetic apparatus


primary quinone acceptor of electrons in PSII


secondary quinone acceptor of electrons


energy dependent component of nonphotochemical quenching of Chl fluorescence


reaction centers


reactive oxygen species




violaxanthin cycle




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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. E. Sofronova
    • 1
  • T. K. Antal
    • 2
  • O. V. Dymova
    • 3
  • T. K. Golovko
    • 3
  1. 1.Institute for Biological Problems of the Cryolithozone, Siberian BranchRussian Academy of SciencesYakutskRussia
  2. 2.Moscow State UniversityMoscowRussia
  3. 3.Institute of Biology, Komi Research Center, Ural BranchRussian Academy of SciencesSyktyvkarRussia

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