Russian Journal of Plant Physiology

, Volume 65, Issue 4, pp 490–497 | Cite as

Substrate Specificity of Acyl-Lipid Δ9-Desaturase from Cyanobacterium sp. IPPAS B-1200, a Cyanobacterium with Unique Fatty Acid Composition

  • A. Yu. Starikov
  • A. A. Usserbaeva
  • K. S. Mironov
  • R. A. Sidorov
  • B. K. Zayadan
  • V. S. Bedbenov
  • M. A. Sinetova
  • D. A. LosEmail author
Research Papers


Cyanobacterium sp. IPPAS B-1200 is characterized by a high content of rare fatty acids (FAs), both myristic (14:0–30%) and myristoleic (14:1Δ9–10%) in the membrane lipids. Thus, short-chain FAs reach 40% of the sum of all FAs in cells, which is unusual for Cyanobacteria. Monounsaturated palmitoleic acids (16:1Δ9) also reach 40% of the sum of the FAs. We determined the complete nucleotide sequence of the genome of this cyanobacterium and found the only gene for the acyl-lipid Δ9-desaturase, desC1. We cloned this gene and characterized its specificity to the length of the substrate using heterologous expression in Escheriсhia coli. The results show that DesC1 nonspecifically generates olefin bond in FAs with a length of 14, 16, and 18 carbon atoms. This finding confirms that all monoesterifed FAs in Cyanobacterium sp. IPPAS B-1200 are generated by one acyl-lipid Δ9-desaturase.


Cyanobacterium genome desaturase fatty acids genes expression 



fatty acid(s)


gas chromatography


mass spectrometry


polyacrylamide gel electrophoresis


polymerase chain reaction


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. Yu. Starikov
    • 1
  • A. A. Usserbaeva
    • 2
  • K. S. Mironov
    • 1
  • R. A. Sidorov
    • 1
  • B. K. Zayadan
    • 2
  • V. S. Bedbenov
    • 1
  • M. A. Sinetova
    • 1
  • D. A. Los
    • 1
    Email author
  1. 1.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of Biology and BiotechnologyAl-Farabi State University of KazakhstanAlmatyKazakhstan

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