AlGLY I gene implicated in salt stress response from halophyte Aeluropus littoralis
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Aeluropus littoralis (Gouan) Parlatore is a rhizomatous perennial monocotyledonous halophyte that withstands environmental stresses. The role of the glyoxalase system, which plays an important role in carbohydrate metabolic process and compatible solutes production, in salt tolerance of A. littoralis was proved to be extremely momentous. Thus in the present study, a GLY I gene was isolated and sequenced from this plant (revealing the partial sequence of AlGLY I), and its expression profiling has been performed in response to salinity and recovery conditions, by fluorescent real-time PCR (qPCR). Experimental samples were prepared separately from shoot and root tissues after 600 mM NaCl treatment, as well as after stress removing. Maximum mRNA expression of GLY I, which was observed after 6 h salt stress in shoot tissue, was 5.9-fold higher compared to the control. Characterization of the partial sequence of AlGLY I gene, containing 896 bp, using publicly available databases demonstrated that the deduced transcripts, encoding 297 amino acids with a 32.5869 kD molecular mass including 5.19 isoelectric points, shared a high homology (~90%) to Oryza sativa GLY I protein. Setaria italica, Sorghum bicolor, Brachypodium distachyon, Triticum aestivum, and Hordeum vulgare with 86, 85, 84, 83 and 78%, respectively, also revealed high homology. The promoter analysis also showed the presence of various stress related CREs, which probably activate the AlGLY I gene transcription under abiotic stress conditions. These results suggested that AlGLY I may be a potentially useful candidate gene for engineering salinity tolerance in cultivated plants.
KeywordsAeluropus littoralis bioinformatics analysis glyoxalase I gene
- GLY I
quantitative real-time polymerase chain reaction
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- 1.Abiotic Stress in Plants—Mechanisms and Adaptations, Shanker, A.K. and Venkateswarlu, B., Eds., Rijeka: InTechOpen, 2011.Google Scholar
- 5.Ahmad, P., Hakeem, K.R., Kumar, A., Ashraf, M., and Akram, N.A., Salt-induced changes in photosynthetic activity and oxidative defense system of three cultivars of mustard (Brassica juncea L.), Afr. J. Biotechnol., 2012, vol. 11, pp. 2694–2703.Google Scholar
- 6.Mustafiz, A., Ghosh, A., Tripathi, A.K., Kaur, C., Ganguly, A.K., Bhavesh, N.S., Tripathi, J.K., Pareek, A., Sopory, S.K., and Singla-Pareek, S.L., A unique Ni2+ dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response, Plant J., 2014, vol. 78, no. 6, pp. 951–963.CrossRefPubMedGoogle Scholar
- 8.Thornalley, P.J., Glyoxalase I—structure, function and a critical role in the enzymatic defence against glycation, Biochem. Soc. Trans., 2003, vol. 31, pp. 1343–1348.Google Scholar
- 14.Hall, T., BioEdit: an important software for molecular biology, GERF Bull. Biosci., 2011, vol. 2, pp. 60–61.Google Scholar
- 25.Kato, N., Akai, M., Zulkifli, L., Matsuda, N., Kato, Y., Goshima, S., Hazama, A., Yamagami, M., Guy, R.H., and Uozumi, N., Role of positively charged amino acids in the M2D transmembrane helix of Ktr/Trk/HKT type cation transporters, Channels, 2007, vol. 1, no. 3, pp. 161–171.CrossRefPubMedGoogle Scholar
- 27.Marchler, B.A., Derbyshire, M.K., Gonzales, N.R., Lu, S., Chitsaz, F., Geer, L.Y., Geer, R.C., He, J., Gwadz, M., Hurwitz, D.I., Lanczycki, C.J., Lu, F., Marchler, G.H., Song, J.S., Thanki, N., et al., CDD: NCBI’s conserved domain database, Nucleic Acids Res., 2015, vol. 43, pp. D222–D226.CrossRefPubMedGoogle Scholar
- 28.Saad, R.B., Zouari, N., Ramdhan, W.B., Azaza, J., Meynard, D., Guiderdoni, E., and Hassairi, A., Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zincfinger “AlSAP” gene isolated from the halophyte grass Aeluropus littoralis, Plant Mol. Biol., 2010, vol. 72, pp. 171–190.CrossRefPubMedGoogle Scholar
- 30.Veena, V.S., Reddy, S.K., and Sopory, S.K., Glyoxalase I from Brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress, Plant J., 1999, vol. 17, pp. 385–395.Google Scholar