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Russian Journal of Plant Physiology

, Volume 64, Issue 1, pp 28–40 | Cite as

Chromosome regions associated with the activity of lipoxygenase in the genome D of Triticum aestivum L. under water deficit

  • M. D. PermyakovaEmail author
  • A. V. Permyakov
  • S. V. Osipova
  • T. A. Pshenichnikova
  • A. A. Shishparenok
  • E. G. Rudikovskaya
  • A. V. Rudikovsky
  • V. V. Verkhoturov
  • A. Börner
Research Papers

Abstract

Quantitative trait loci (QTLs) associated with the phenotypic expression of the activity of different forms of lipoxygenase (LOX) under water deficit were detected in the chromosomes of the D-genome using intogression lines of common wheat Triticum aestivum L. Chinese Spring (Synthetic 6x). QTL associated with the activity of seed soluble LOX was identified on the short arm of chromosome 4D. The activity of membranebound form of enzyme in the seedlings was mapped to the short arm, while that of a soluble form was on the long arm of chromosome 5D. Two regions responsible for the activity of soluble LOX in the leaves were found on the short arm of chromosome 2D. Three QTLs associated with the activities of chloroplast LOXs were found on the same chromosome: the activity of the soluble form was linked to Xgwm261 and Xgwm539 markers, and the membrane form to Xgdm93 marker. QTLs for the activities of both soluble and membrane-bound LOX in the leaves were identified in the centromeric region of chromosome 7D. The activities of two membrane enzymes in the leaves were linked to Xgdm130 marker on the short arm of this chromosome. Loci associated with the activity of different LOX forms colocalized with QTLs for the shoot mass, gas exchange parameters, chlorophyll fluorescence, content of photosynthetic pigments, and grain productivity of wheat. A correlation between these parameters and the LOX activity was detected and it was shown that various forms of the enzyme were differentially involved in the adaptation of wheat plants to water deficit. The current paper discusses their presumed physiological role.

Keywords

Triticum aestivum quantitative trait loci water deficit lipid metabolism lipoxygenase activity gas exchange chlorophyll fluorescence photosynthetic pigments drought 

Abbreviations

WD

water deficit

SL

stem length

JA

jasmonic acid

IL

introgression lines

Car

carotenoids

leafMLOX

membrane-bound LOX in the leaves

leafSLOX

soluble LOX in the leaves

QTL

quantitative trait locus

LOX

lipoxygenase

GWE

grain weight per ear

SM

shoot mass

NWS

normal water supply

Phr

CO2 assimilation rate

PUFA

polyunsaturated fatty acids

sdlMLOX

membrane-bound LOX in the seedlings

sdlSLOX

soluble LOX in the seedlings

SN

spikelet number per spike

seedSLOX

soluble LOX in the seeds

TI

tolerance index

TR

transpiration rate

ETR

electron transport rate

SC

stomatal conductance

Chl

chlorophyll

ChlMLOX

membrane-bound LOX in the chloroplasts

ChlSLOX

soluble LOX in the chloroplasts

WUE

water use efficiency

Y(II)

effective photochemical quantum yield of photosystem II

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • M. D. Permyakova
    • 1
    Email author
  • A. V. Permyakov
    • 1
  • S. V. Osipova
    • 1
    • 4
  • T. A. Pshenichnikova
    • 2
  • A. A. Shishparenok
    • 1
  • E. G. Rudikovskaya
    • 1
  • A. V. Rudikovsky
    • 1
  • V. V. Verkhoturov
    • 3
  • A. Börner
    • 5
  1. 1.Siberian Institute of Plant Physiology and Biochemistry, Siberian BranchRussian Academy of SciencesIrkutskRussia
  2. 2.Federal Research Center, Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  3. 3.Irkutsk National Research Technical UniversityIrkutskRussia
  4. 4.Irkutsk State UniversityIrkutskRussia
  5. 5.Leibniz Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany

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