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Russian Journal of Plant Physiology

, Volume 63, Issue 4, pp 566–574 | Cite as

pH and CO2 effects on Coelastrella (Scotiellopsis) rubescens growth and metabolism

  • G. S. Minyuk
  • E. S. Chelebieva
  • I. N. Chubchikova
  • N. V. Dantsyuk
  • I. V. Drobetskaya
  • E. G. Sakhon
  • O. B. Chivkunova
  • K. A. Chekanov
  • E. S. Lobakova
  • R. A. Sidorov
  • A. E. SolovchenkoEmail author
Research Papers

Abstract

We studied effects of рН and СО2 enrichment on the physiological condition and biochemical composition of a carotenogenic microalga Coelastrella (Scotiellopsis Vinatzer) rubescens Kaufnerová et Eliás (Scenedesmaceae, Sphaeropleales, Chlorophyceae), a promising source of natural astaxanthin. The microalga was grown at a constant pH (5, 6, 7 or 8) maintained by direct СО2 injection. The air-sparged culture served as the control. Cell division rate and size, dry biomass productivity, the rates of nitrogen and phosphorus uptake as well as photosynthetic pigment and total lipid content and fatty acid composition were followed. С. rubescens possessed a narrow-range рН tolerance (the optimum рН 6–7). Under these conditions, the highest values of the maximum (1.0–1.1 1/day) and average (0.3–0.35 1/day) specific growth rate, chlorophyll а (4.8–4.9%) and total carotenoid dry weight percentages (1.7–1.8%) were recorded. Cell lipid fatty acid unsaturation index (1.851) and polyunsaturated fatty acid percentage (36–39%) and С18:3 ω3/С18:1 ω9 ratio (3.8–4.5) were also the highest under these conditions. A decline of рН to 5 brought about severe stress manifesting itself as a cell division cessation, photosynthetic apparatus reduction, two-fold increase in cell volume, accumulation of dry weight and lipids and a considerable decline in fatty acid unsaturation. Cultivation of С. rubescens without СО2 enrichment resulted in a rapid alkalization of the medium to рН 9.5–10.5 impairing the physiological condition of the cells. Reasons of the deteriorative effects of suboptimal pH values on the physiological condition of C. rubescens are discussed.

Keywords

Coelastrella (Scotiellopsis) rubescens СО2 cultivation fatty acids growth rate рН pigments 

Abbreviations

Ast

astaxanthin

Car

carotenoids

Chl а, Chl b

chlorophylls а and b

FA

fatty acids

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • G. S. Minyuk
    • 1
  • E. S. Chelebieva
    • 1
  • I. N. Chubchikova
    • 1
  • N. V. Dantsyuk
    • 1
  • I. V. Drobetskaya
    • 1
  • E. G. Sakhon
    • 1
  • O. B. Chivkunova
    • 2
  • K. A. Chekanov
    • 2
  • E. S. Lobakova
    • 2
  • R. A. Sidorov
    • 3
  • A. E. Solovchenko
    • 2
    Email author
  1. 1.Kovalevsky Institute of Biology of Southern SeasSevastopolRussia
  2. 2.Biological facultyLomonosov State UniversityMoscowRussia
  3. 3.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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