Russian Journal of Plant Physiology

, Volume 63, Issue 3, pp 338–348 | Cite as

Dominant form of cationic peroxidase from sorghum roots

  • E. V. Dubrovskaya
  • N. N. Pozdnyakova
  • V. S. Grinev
  • A. Yu. Muratova
  • S. N. Golubev
  • A. D. Bondarenkova
  • O. V. Turkovskaya
Research Papers


A dominant form of cationic peroxidase (PO-2) was isolated from sorghum (Sorghum bicolor L. Moench) roots and purified to electrophoretically homogeneous state. The enzyme is a monomer with mol wt of 49.7 kD. The optimum pH and the main catalytic constants (KM, V max, k cat) were determined for oxidation of the main substrates including Н2О2, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 2,7-diaminofluorene, syringaldazine, 2,6-dimethoxyphenol, and o-dianisidine. The KM values increased in the sequence: H2O2 < 2,7-diaminofluorene < ABTS < o-dianisidine, whereas the maximum turnover number (93.9 s–1) was found for 2,7-diaminofluorene. Based on the analysis of molecular and catalytic properties of the enzyme, it was proven that PO-2 is a typical cationic plant peroxidase. Polycyclic aromatic hydrocarbons (phenanthrene, anthracene, fluorene), 2,2'-diphenic acid, and Ni ions had no significant influence on the activity of PO-2. The enzyme was inhibited by p-aminobenzoic acid, NaN3, 1-naphthol, 9,10-anthraquinone, and 9,10-phenanthrenequinone. In the presence of NaN3, 1-naphthol, and 9,10-phenanthrenequinone, a mixed competitive/noncompetitive type of inhibition was noted. The peroxidase PO-2 was found to oxidize synthetic anthraquinone dyes, phenanthrene, and some oxygenated derivatives of polycyclic aromatic hydrocarbons (9-phenanthrol; 1-naphthol; and 1-hydroxy-2-naphthoic, salicylic, and 2,2'-diphenic acids), which indirectly confirms the coupled plant–microbial metabolism of these compounds in the root zone of sorghum. The results indicate that 9,10-phenanthrenequinone and 2,2'-diphenic acid are the products of peroxidase-catalyzed oxidation of 9-phenanthrol.


Sorghum bicolor cationic peroxidase catalytic properties polycyclic aromatic hydrocarbons (PAH) PAH metabolites anthraquinone dyes 



Acid Blue 62




alizarin red


Basic Blue 22


polycyclic aromatic hydrocarbons


cationic peroxidase


Reactive Blue 4


4-hydroxy-3,5-dimethoxybenzaldehyde azine


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • E. V. Dubrovskaya
    • 1
  • N. N. Pozdnyakova
    • 1
  • V. S. Grinev
    • 1
  • A. Yu. Muratova
    • 1
  • S. N. Golubev
    • 1
  • A. D. Bondarenkova
    • 1
  • O. V. Turkovskaya
    • 1
  1. 1.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia

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