Russian Journal of Plant Physiology

, Volume 63, Issue 1, pp 124–131 | Cite as

Morphological changes and increase of resistance to oxidative stress by overexpression of the LebZIP2 gene in Nicotiana benthamiana

  • E. S. Seong
  • J. H. Yoo
  • N. J. Kim
  • J. H. Choi
  • J. G. Lee
  • B. K. Ghimire
  • I. M. Chung
  • C. Y. Yu
Research Papers


The tomato bZIP2-encoding gene was inserted into the Nicotiana benthamiana genome using Agrobacterium-mediated transformation to characterize resistance to oxidative stress and two herbicides, glyphosate and paraquat. We produced transgenic tobacco plants using the LebZIP2 gene, which were then utilized to examine salt stress and herbicide resistance through oxidative mechanisms. Transgenic LebZIP2-overexpressing plants were examined using specific primers for selection marker genes (PCR using genomic DNA) and target genes (RT-PCR). Based on microscopic examination, we observed an increase in leaf thickness and cell number in transgenic plants. The electrolyte leakage of leaves suggested that LebZIP2-overexpressing lines were weak tolerant to NaCl stress and resistant to methyl viologen. During our analysis, transgenic lines were exposed to different herbicides. Transgenic plants showed an increased tolerance based on visual injury, as well as an increased biomass. Based on these results, the LebZIP2 gene may be involved in oxidative stress tolerance and cell development in plants.


Nicotiana benthamiana LebZIP2 oxidative stress NaCl biomass methyl viologen glyphosate paraquat 



basic region/leucine zipper


methyl viologen


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • E. S. Seong
    • 1
  • J. H. Yoo
    • 2
  • N. J. Kim
    • 2
  • J. H. Choi
    • 2
  • J. G. Lee
    • 3
  • B. K. Ghimire
    • 4
  • I. M. Chung
    • 4
  • C. Y. Yu
    • 2
  1. 1.Bioherb Research InstituteKangwon National UniversityChuncheonSouth Korea
  2. 2.Department of Bioconvergence Science and Technology, College of Agriculture and Life ScienceKangwon National UniversityChuncheonSouth Korea
  3. 3.Hwajin CosmeticsHongcheonSouth Korea
  4. 4.Department of Applied BioscienceKonkuk UniversitySeoulSouth Korea

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