Russian Journal of Plant Physiology

, Volume 62, Issue 6, pp 823–829 | Cite as

Differences in photosynthesis and terpene content in leaves and roots of wild-type and transgenic Arabidopsis thaliana plants

  • J. S. BlanchEmail author
  • J. Peñuelas
  • J. Llusià
  • J. Sardans
  • S. M. Owen
Research Papers


We investigated the hypotheses that two different varieties of Arabidopsis thaliana show differences in physiology and terpene production. The two varieties of A. thaliana used in this study were wild-type (WT) and transgenic line (CoxIV-FaNES I) genetically modified to emit nerolidol with linalool/nerolidol synthase (COX). Photosynthetic rate, electron transport rate, fluorescence, leaf volatile terpene contents and root volatile terpene contents were analyzed. For both types, we found co-eluting α-pinene+β-ocimene, limonene, and humulene in leaves; and in the roots we found co-eluting α-pinene+β-ocimene, sabinene+β-pinene, β-myrcene, limonene, and humulene. At the end of the growing cycle, COX plants tended to have lower pools of terpene compounds in their leaves, with 78.6% lower photosynthesis rates and 30.8% lower electron transport rates, compared with WT plants at that time. The maximal photochemical efficiency F v/F m was also significantly lower (25.5%) in COX plants, indicating that these varieties were more stressed than WT plants. However, COX plants had higher (239%) root terpene contents compared to WT plants. COX plants appear to favor root production of volatile terpenes rather than leaf production. Thus we conclude that there were significant differences between COX and WT plants in terms of terpenoid pools, stress status and physiology.


Arabidopsis thaliana FaNES I leaf terpene contents root terpene contents photosynthesis 



CO2 uptake


transgenic line (CoxIV-FaNES I)


electron transport rate


maximum photochemical efficiency of PSII


actual photochemical efficiency of PSII


stomatal conductance


terpene synthases


volatile organic compounds




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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • J. S. Blanch
    • 1
    • 2
    Email author
  • J. Peñuelas
    • 1
    • 2
  • J. Llusià
    • 1
    • 2
  • J. Sardans
    • 1
    • 2
  • S. M. Owen
    • 1
    • 2
    • 3
  1. 1.CSIC, Global Ecology UnitCREAF-CSIC-UABCataloniaSpain
  2. 2.CREAFCataloniaSpain
  3. 3.Centre for Ecology and Hydrology (CEH)PenicuikUK

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