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Russian Journal of Plant Physiology

, Volume 62, Issue 4, pp 499–506 | Cite as

Antioxidant enzyme activity and osmolyte content in winter cereal seedlings under hardening and cryostress

  • Yu. E. Kolupaev
  • N. I. Ryabchun
  • A. A. Vayner
  • T. O. Yastreb
  • A. I. Oboznyi
Research Papers

Abstract

Activities of antioxidant enzymes and the osmolyte contents in seedlings of winter rye (Secale cereale L.), soft (Triticum aestivum L.) and durum (T. durum L.) wheat, and barley (Hordeum vulgare L.) grown at 20°C (control) or after 7-day cold hardening at 2°C and/or 5-hour freezing at −6°C were investigated. It was found that nonhardened rye seedlings differed from those of other cereals by their ability to survival after freezing at −6°C and higher activity of guaiacol peroxidase (GPO) and high content of proline. Hardening induced the increase in the frost tolerance of all cereals under study, and the resistance of rye and soft wheat was found to be significantly higher than that of durum wheat and barley. Rye and soft wheat exhibited more profound tolerance to oxidative damages as well, and it was expressed in lesser increase in the MDA content after freezing. In the course of hardening, detectable increase in the activities of GPO and catalase (CAT), as well as the contents of proline and soluble carbohydrates, was observed in seedlings of all cereals under study. In barley, the activity of superoxide dismutase (SOD) increased to the highest extent under these conditions. After freezing of both hardened and nonhardened seedlings, higher activities of all tested antioxidant enzymes were revealed in rye and soft wheat as compared to those in durum wheat and barley. In this case, hardened rye and soft wheat seedlings after freezing displayed increased content of proline. All these results lead to the conclusion that the high content of proline and activity of GPO observed in rye seedlings may determine their increased constitutive frost tolerance, whereas high tolerance of hardened soft wheat seedlings is primarily associated with accumulation of low-molecular-weight protectors, such as sugars and proline, and, to some extent, with the increased activity of antioxidant enzymes.

Keywords

Secale cereale Triticum aestivum T. durum Hordeum vulgare frost tolerance hardening antioxidant enzymes soluble carbohydrates proline 

Abbreviations

GPO

guiaicol peroxidase

CAT

catalase

SOD

superoxide dismutase

TBA

2-thiobarbituric acid

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References

  1. 1.
    Trunova, T.I., Rastenie i nizkotemperaturnyi stress. 64-e Timiryazevskoe chtenie (Plant and Low Temperature Stress, the 64th Timiryazev Lecture), Moscow: Nauka, 2007.Google Scholar
  2. 2.
    Theocharis, A., Clement, C., and Barka, E.A., Physiological and molecular changes in plants grown at low temperatures, Planta, 2012, vol. 235, pp. 1091–1105.PubMedCrossRefGoogle Scholar
  3. 3.
    Grabelnych, O.I., Sumina, O.N., Funderat, S.P., Pobezhimova, T.P., Voinikov, V.K., and Kolesnichenko, A.V., The distribution of electron transport between the main cytochrome and alternative pathways in plant mitochondria during short-term cold stress and cold hardening, J. Therm. Biol., 2004, vol. 29, pp. 165–175.CrossRefGoogle Scholar
  4. 4.
    Foyer, C.H. and Noctor, G., Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications, Antioxid. Redox Signal., 2009, vol. 11, pp. 861–906.PubMedCrossRefGoogle Scholar
  5. 5.
    Pradedova, E.V., Isheeva, O.D., and Salyaev, R.K., Classification of the antioxidant defense system as the ground for reasonable organization of experimental studies of the oxidative stress in plants, Russ. J. Plant Physiol., 2011, vol. 58, pp. 210–217.CrossRefGoogle Scholar
  6. 6.
    Baek, K.H. and Skinner, D.Z., Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines, Plant Sci., 2003, vol. 165, pp. 1221–1227.CrossRefGoogle Scholar
  7. 7.
    Naraikina, N.V., Sin’kevich, M.S., Demin, I.N., Selivanov, A.A., Moshkov, I.E., and Trunova, T.I., Changes in the activity of superoxide dismutase isoforms in the course of low-temperature adaptation in potato plants of wild type and transformed with Δ12-acyl-lipid desaturase gene, Russ. J. Plant Physiol., 2014, vol. 61, pp. 332–338.CrossRefGoogle Scholar
  8. 8.
    Janda, T., Szalai, G., Rios-Gonzalez, K., Veisz, O., and Paldi, E., Comparative study of frost tolerance and antioxidant activity in cereals, Plant Sci., 2003, vol. 164, pp. 301–306.CrossRefGoogle Scholar
  9. 9.
    Apostolova, P., Yordanova, R., and Popova, L., Response of antioxidative defence system to low temperature stress in two wheat cultivars, Gen. Appl. Plant Physiol., 2008, vol. 34, pp. 281–294.Google Scholar
  10. 10.
    Dzhavadian, N., Karimzade, G., Mafuzi, S., and Ganati, F., Cold-induced changes of enzymes, proline, carbohydrates, and chlorophyll in wheat, Russ. J. Plant Physiol., 2010, vol. 57, pp. 540–547.CrossRefGoogle Scholar
  11. 11.
    Lukatkin, A.S., Contribution of oxidative stress to the development of cold-induced damage to leaves of chilling-sensitive plants. 2. The activity of antioxidant enzymes during plant chilling, Russ. J. Plant Physiol., 2002, vol. 49, pp. 782–788.CrossRefGoogle Scholar
  12. 12.
    Sin’kevich, M.S., Deryabin, A.N., and Trunova, T.I., Characteristics of oxidative stress in potato plants with modified carbohydrate metabolism, Russ. J. Plant Physiol., 2009, vol. 56, pp. 168–174.CrossRefGoogle Scholar
  13. 13.
    Kolupaev, Yu.E. and Karpets, Yu.V., Formirovanie adaptivnykh reaktsii rastenii na deistvie abioticheskikh stressorov (Formation of Adaptive Responses of Plants to Abiotic Stressors), Kiev: Osnova, 2010.Google Scholar
  14. 14.
    Liang, X., Zhang, L., Natarajan, S.K., and Becker, D.F., Proline mechanisms of stress survival, Antioxid. Redox Signal., 2013, vol. 19, pp. 998–1011.PubMedCentralPubMedCrossRefGoogle Scholar
  15. 15.
    Vagujfalvi, A., Kerepesi, I., Galiba, G., Tischner, T., and Sutka, J., Frost hardiness depending on carbohydrate changes during cold acclimation in wheat, Plant Sci., 1999, vol. 144, pp. 85–92.CrossRefGoogle Scholar
  16. 16.
    Burbulis, N., Jonytiene, V., Kupriene, R., and Blinstrubiene, A., Changes in proline and soluble sugars content during cold acclimation of winter rapeseed shoots in vitro, J. Food Agricult. Environ., 2011, vol. 9, pp. 371–374.Google Scholar
  17. 17.
    Radyukina, N.L., Shashukova, A.V., Makarova, S.S., and Kuznetsov, Vl.V., Exogenous proline modifies differential expression of superoxide dismutase genes in UV-B-irradiated Salvia officinalis plants, Russ. J. Plant Physiol., 2011, vol. 58, pp. 51–59.CrossRefGoogle Scholar
  18. 18.
    Carvalho, K., Campos, M.K., Domingues, D.S., Pereira, L.F., and Vieira, L.G., The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo, Mol. Biol. Rep., 2013, vol. 40, pp. 3269–3279.PubMedCrossRefGoogle Scholar
  19. 19.
    Kartashov, A.V., Radyukina, N.L., Ivanov, Yu.V., Pashkovskii, P.P., Shevyakova, N.I., and Kuznetsov, Vl.V., Role of antioxidant systems in wild plant adaptation to salt stress, Russ. J. Plant Physiol., 2008, vol. 55, pp. 463–468.CrossRefGoogle Scholar
  20. 20.
    Samygin, G.A., Rapid determination of the relative hardiness of wheat samples by freezing of germinated seeds, Metody opredeleniya morozostoikosti rastenii (Methods for Determination of Frost Resistance), Moscow: Nauka, 1967, pp. 77–84.Google Scholar
  21. 21.
    Merzlyak, M.N., Pogosyan, S.I., Yuferova, S.G., and Shevyreva, V.A., The Use of 2-Thiobarbituric Acid in the Study of Lipid Peroxidation in Plant Tissues, Biol. Nauki, 1978, no. 9, pp. 86–94.Google Scholar
  22. 22.
    Karpets, Yu.V., Kolupaev, Yu.E., Lugovaya, A.A., and Oboznyi, A.I., Effect of jasmonic acid on the pro-/antioxidant system of wheat coleoptiles as related to hyperthermia tolerance, Russ. J. Plant Physiol., 2014, vol. 61, pp. 339–346.CrossRefGoogle Scholar
  23. 23.
    Alscher, R.G., Erturk, N., and Heath, L.S., Role of superoxide dismutases (SODs) in controlling oxidative stress in plants, J. Exp. Bot., 2002, vol. 53, pp. 1331–1341.PubMedCrossRefGoogle Scholar
  24. 24.
    Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.PubMedCrossRefGoogle Scholar
  25. 25.
    Zhao, K., Fan, H., Zhou, S., and Song, J., Study on the salt and drought tolerance of Suaeda salsa and Kalanchoe claigremontiana under iso-osmotic salt and water stress, Plant Sci., 2003, vol. 165, pp. 837–844.CrossRefGoogle Scholar
  26. 26.
    Bates, L.S., Walden, R.P., and Tear, G.D., Rapid determination of free proline for water stress studies, Plant Soil, 1973, vol. 39, pp. 205–210.CrossRefGoogle Scholar
  27. 27.
    Wanner, L.A. and Junttila, O., Cold-induced freezing tolerance in Arabidopsis, Plant Physiol., 1999, vol. 120, pp. 391–399.PubMedCentralPubMedCrossRefGoogle Scholar
  28. 28.
    Radyukina, N.L., Kartashov, A.V., Ivanov, Yu.V., Shevyakova, N.I., and Kuznetsov, Vl.V., Functioning of defense systems in halophytes and glycophytes under progressing salinity, Russ. J. Plant Physiol., 2007, vol. 54, pp. 806–815.CrossRefGoogle Scholar
  29. 29.
    Michaeli, R., Philosoph-Hadas, S., Riov, J., Shahak, Y., Ratner, K., and Meir, S., Chilling-induced leaf abscission of Ixora coccinea plants. III. Enhancement by high light via increased oxidative processes, Physiol. Plant., 2001, vol. 113, pp. 338–345.PubMedCrossRefGoogle Scholar
  30. 30.
    Konstantinova, T., Parvanova, D., Atanassov, A., and Djilianov, D., Freezing tolerant tobacco, transformed to accumulate osmoprotectants, Plant Sci., 2002, vol. 163, pp. 157–164.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • Yu. E. Kolupaev
    • 1
  • N. I. Ryabchun
    • 2
  • A. A. Vayner
    • 1
  • T. O. Yastreb
    • 1
  • A. I. Oboznyi
    • 1
  1. 1.Dokuchaev National Agrarian UniversityKharkivUkraine
  2. 2.Yur’ev Institute of Plant BreedingNational Academy of Agrarian Sciences of UkraineKharkivUkraine

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