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Russian Journal of Plant Physiology

, Volume 62, Issue 3, pp 360–366 | Cite as

Transgenic tomato overexpressing ath-miR399d improves growth under abiotic stress conditions

  • N. Gao
  • X. M. Qiang
  • B. N. Zhai
  • J. Min
  • W. M. ShiEmail author
Research Papers

Abstract

Phosphorus (P) is an essential element required for plant growth and development. Abiotic stresses, such as cold and P deficiency cause adverse effects on growth of plants, inhibit accumulation or translocation of mineral nutrients such as P, and often constrain the productivity and quality of crops. miR399 as a long-distance signal controls systemic phosphate homeostasis by modulating phosphate uptake and transport. However, overexpression of miR399 causes P toxicity and results in retarded growth. Thus, we constructed a transgenic tomato (Solanum lycopersicum L., C1), which contains foreign gene ath-miR399d under the control of rd29A promoter. In the transgenic C1 expression of the miR399d gene showed a stress-inducible pattern. Results showed that miR399d was moderately up-regulated in response to salinity and cold stresses. In soil, dry weight was enhanced in transgenic tomato under low temperature and P deficiency conditions. The transgenic tomato has potential to improve growth when it is exposed to other abiotic stresses such as salinity and drought.

Keywords

Solanum lycopersicum transgenic tomato microRNA399 abiotic stress 

Abbreviations

24/16

plants grown in chamber at 24°C for day-time and at 16°C for night-time

24/24

plants grown in chamber at constant 24°C

P0

150 mg P2O5/kg soil as KH2PO4 added into soil

P1

300 mg P2O5/kg soil as KH2PO4 added into soil

Pi

inorganic phosphorus

RT-qPCR

real-time quantitative reverse transcription PCR

WT

wild type

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • N. Gao
    • 1
  • X. M. Qiang
    • 1
    • 2
  • B. N. Zhai
    • 2
  • J. Min
    • 1
  • W. M. Shi
    • 1
    Email author
  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingChina
  2. 2.College of Resource and EnvironmentNorthwest Agriculture and Forestry UniversityBeijingChina

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