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Russian Journal of Plant Physiology

, Volume 61, Issue 5, pp 714–717 | Cite as

Characterization of proteases secreted by leek roots

  • B. Adamczyk
Brief Communications
  • 107 Downloads

Abstract

Some characteristics of root-secreted proteases were studied. We measured their molecular weights and mechanism of BSA digestion in comparison to endogenous root proteases. We related these studies to culture medium N composition. The seedlings of Allium porrum L. (cv. Bartek) were cultivated on MS medium, MS without inorganic nitrogen (MS-IN), and MS without IN, but with 0.1% casein (MS-IN + 0.1% casein). Electrophoretic study showed that root-secreted proteases had one isoform with a mol wt of 45 kD, regardless of medium N composition. Difference in molecular weights of root-secreted proteases and endogenous root proteases active under used conditions (>66 kD) provide us another strong evidence that root-secreted proteases were not just leaking from the roots, but they were secreted. Proteases exuded by roots degraded BSA in a similar way as endogenous proteases, with only one SDS-PAGE-detectable product of degradation. Our results may be a powerful tool in the extraction and purification of these enzymes and also in proteomic studies.

Keywords

Allium porrum organic nitrogen plant nitrogen nutrition protease secretion proteolytic activity 

Abbreviations

MS-IN

MS without inorganic nitrogen

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  1. 1.Department of Plant Cytology and Cytochemistry, Institute of Plant Physiology, Cytology and CytogeneticUniversity of ŁódźŁódźPoland
  2. 2.Finnish Forest Research InstituteVantaa UnitVantaaFinland

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