Russian Journal of Plant Physiology

, Volume 60, Issue 3, pp 431–436 | Cite as

Quantitative analysis of IAA in DR5::GUS transgenic arabidopsis plants

  • G. A. Pozhvanov
  • A. L. Shavarda
  • S. S. Medvedev


In the study of auxin transport, transgenic constructs, including DR5::GUS, are widely used for visualization of phytohormone localization. Previously we proposed a method for quantitative evaluation of the IAA content by histochemical staining for glucuronidase activity. In this work, this method was complemented by quantitative data on the content of IAA in plants obtained by gas chromatography-mass spectrometry (GC/MS), which allowed more accurate characterization of the lateral IAA gradient arising at the Arabidopsis thaliana (L.) Heynh (ecotype Columbia 0) root gravistimulation. Applied method of IAA analysis, combining GC/MS and histochemistry, can be used for quantitatification of the other plant hormone distribution in transgenic plants with the GUS reporter.


Arabidopsis thaliana IAA redistribution GUS reporter gas chromatography mass-spectrometry digital image analysis 



gas chromatography with mass-selective detection


reporter enzyme β-D-glucuronidase from Escherichia coli


color model where the combination of red, green, and blue color (R, G, B) produces the final color of digital image pixel


trimethylsilyl fragment (Si(CH3)3)


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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • G. A. Pozhvanov
    • 1
    • 2
  • A. L. Shavarda
    • 1
    • 2
  • S. S. Medvedev
    • 1
  1. 1.Department of Plant Physiology and BiochemistrySt. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Komarov Institute of BotanyRussian Academy of SciencesSt. PetersburgRussia

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