Effects of abscisic acid on the contents of polyamines and proline in common bean plants under salt stress
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The effects of ABA treatment on the contents of polyamines (PAs) and proline (Pro) in the glycophyte Phaseolus vulgaris L. during plant adaptation to salt stress were studied. Two-week-old common bean seedlings grown in the phytotron chamber on the Jonson nutrient medium were subjected to salinity for 6 days by one-time NaCl addition to medium up to final concentrations of 50 and 100 mM. During first three days of salinity, the root system was daily treated with ABA (1, 5, 10, or 50 μM) for 30 min. Salt stress (100 mM NaCl) elevated the level of endogenous ABA, increased the content of Pro 14-fold, reduced sharply the content of free PAs (putrescine, spermidine, spermine, and cadaverine), and the accumulation of 1,3-diaminopropan, a product of oxidation of high-molecular PAs. Common bean plant treatment with 1 μM ABA weakened the adverse effects of salt stress (100 mM NaCl), which was manifested in the maintenance of plant growth, stimulation of chlorophyll (a and b) and carotenoid accumulation, a stabilization of water and Na+ balance. Seedling treatment with ABA suppressed NaCl-induced Pro and intracellular ABA accumulation and restored the levels of putrescine and spermidine. The content of spermine in the leaves of plants subjected to salt stress and treated with ABA was approximately threefold higher than in control plants, whereas the content of cadaverine increased under similar conditions more than fivefold. Simultaneously, the contents of 1,3-diaminopropan and malondialdehyde as well as activity of superoxide dismutase were reduced, which indicates a weakening of oxidative stress, one of the possible causes of defensive ABA effects against salt stress. In addition, the suppression by exogenous ABA of Pro accumulation and stimulation of PA content under salt stress confirm indirectly our hypothesis that ABA is involved in the coordinated regulation of two biosynthetic pathways, Pro and PA formation, which use a common precursor, glutamate, and play an important protective role during stress in plants.
KeywordsPhaseolus vulgaris salt stress ABA polyamines proline
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- 2.Kuznetsov, Vl.V. and Shevyakova, N.I., Proline under Stress: Biological Role, Metabolism, and Regulation, Russ. J. Plant Physiol., 1999, vol. 46, pp. 274–288.Google Scholar
- 3.Rushton, D.L., Tripathi, P., Rabara, R.C., Ringler, P., Boken, A.K., Langum, T.J., Smidt, L., Boomsma, D.D., Emme, N.J., Chen, X., Finer, J.J., Shen, Q.J., and Rushton, P.J., WRKY Transcription Factors: Key Components in Abscisic Acid Signaling, Plant Biotechnol. J., 2011, doi 101111/j.1467-7652.2011.00634.Google Scholar
- 4.Shevyakova, N.I., Musatenko, L.I., Stetsenko, L.A., Rakitin, V.Yu., Vedenicheva, N.P., Voitenko, L.V., and Kuznetsov, Vl.V., Effects of Salinity on Growth and Phytohormones and Polyamines Contents in Phaseolus vulgaris L. Plants, Fiziol. Biokhim. Kul’t. Rast., 2010, vol. 42, pp. 483–490.Google Scholar
- 5.Vedenicheva, N.P., Voitenko, L.V., Musatenko, L.I., Stetsenko, L.A., and Shevyakova, N.I., Effects of Salinity on Phytohormones Contents in Mesembryanthemum crystallinum L. Leaves, Vestn. Khar’k. Nats. Univ. im. V.N. Karazina, Ser. Biol., 2010, no. 3, pp. 30–36.Google Scholar
- 7.Urano, K., Yoshiba, Y., Nanjo, T., Igarashi, Y., Seki, M., Sekiguchi, F., Yamaguchi-Shinozaki, K., and Shinozaki, K., Characterization of Arabidopsis Genes Involved in Biosynthesis of Polyamines in Abiotic Stress Responses and Developmental Stages, Plant Cell Environ., 2003, vol. 26, pp. 1917–1926.CrossRefGoogle Scholar
- 9.Strizhov, N., Abraham, E., Okresz, L., Blickling, S., Zilberstein, A., Schell, J., Koncz, C., and Szabados, L., Differential Expression of Two P5CS Genes Controlling Proline Accumulation during Salt-Stress Requires ABA and Is Regulated by ABA1, ABI1 and AXR2 in Arabidopsis, Plant J., 1997, vol. 12, pp. 557–569.PubMedCrossRefGoogle Scholar
- 10.Hare, P.D., Cress, W.A., and Staden, J., Proline Synthesis and Degradation: A Model System for Elucidating Stress-Related Signal Transduction, J. Exp. Bot., 1999, vol. 50, pp. 413–434.Google Scholar
- 14.Shlyk, A.A., Determination of Chlorophyll and Carotenoid Contents in Green Leaf Extracts, Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Pavlinova, O.A., Ed., Moscow: Nauka, 1971, pp. 154–170.Google Scholar
- 19.Kuznetsov, Vl.V., Shorina, M.V., Aronova, E.E., Stetsenko, L.A., Rakitin, V.Yu., and Shevyakova, N.I., NaCl- and Ethylene-Dependent Cadaverine Accumulation and Its Possible Protective Role in the Adaptation of the Common Ice Plant to Salt Stress, Plant Sci., 2007, vol. 172, pp. 363–370.CrossRefGoogle Scholar
- 25.Kholodova, V.P., Meshcheryakov, A.B., Rakitin, V.Yu., Karyagin, V.V., and Kuznetsov, Vl.V., Hydraulic Signal as the “Primary Messenger of Water Deficit” in Plants under Salt Stress, Dokl. Akad. Nauk, 2006, vol. 407, pp. 282–285.Google Scholar