Advertisement

Russian Journal of Plant Physiology

, Volume 59, Issue 3, pp 398–405 | Cite as

Changes in cytokinin, auxin, and abscisic acid contents in wheat seedlings treated with the growth regulator Stifun

  • O. I. Yakhin
  • A. A. Lubyanov
  • I. A. Yakhin
Applied Aspects

Abstract

The growth regulator Stifun at all concentrations tested (0.033, 0.33, 3.3, and 33 mg/L) affected the hormonal status of wheat seedlings (Triticum aestivum L., cv. Zhnitsa) and stimulated plant growth. This was evident in activation of cell division and elongation, as well as in the increase in shoot and root length, water content, and dry weight. Effects of Stifun on roots and shoots depended on concentration. Application of Stifun at the optimal growth-stimulating concentration (0.033 mg/L) elevated the levels of zeatin, zeatin riboside, dihydrozeatin riboside, isopentenyladenosine, and IAA in roots of 2-day-old seedlings, but reduced the ABA content. The levels of ABA, IAA, dihydrozeatin, and dihydrozeatin riboside in shoots increased, while the levels of zeatin riboside, isopentenyl adenine, and isopentenyladenosine decreased. The results indicate that the hormonal system plays a part in the plant response to growth-stimulating action of Stifun.

Keywords

Triticum aestivum growth regulator Stifun cytokinins zeatin zeatin riboside dihydrozeatin dihydrozeatin riboside isopentenyl adenine isopentenyladenosine IAA ABA mitotic index 

Abbreviations

CK

cytokinin

DHZ

dihydrozeatin

DHZR

dihydrozeatin riboside

ELISA

enzyme immunosorbent analysis

IP

isopentenyl adenine

IPA

isopentenyladenosine

MI

mitotic index

PBS

phosphate buffer saline supplemented with Tween-20

Z-zeatin

ZR-zeatin riboside

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Prusakova, L.D., Malevannaya, N.N., Belopukhov, S.L., and Vakulenko, V.V., Plant Growth Regulators with Antistress and Immunoprotective Properties, Agrokhimiya, 2005, no. 11, pp. 76–86.Google Scholar
  2. 2.
    Yakhin, I.A., Vakhitov, V.A., Isaev, R.F., and Yakhin, O.I., The way for Improving of Crop Productivity, RF Patent no. 2076603, Byull. Izobret., 1997, no. 10.Google Scholar
  3. 3.
    Yakhin, I.A., Yakhin, O.I., and Isaev, R.F., Effect of Stifun on Growth and Productivity of Spring Wheat, Dokl. Ross. Akad. S.-kh. Nauk, 1999, no. 6, pp. 8–10.Google Scholar
  4. 4.
    Yakhin, I.A., Yakhin, O.I., and Vakulenko, V.V., Stifun-A New Plant Growth Regulator with Fungicidal Activity, Zashchita Karantin Rast., 2000, no. 4, p. 19.Google Scholar
  5. 5.
    Arkhipova, T.N., Veselov, S.U., Melentiev, A.I., Martynenko, E.V., and Kudoyarova, G.R., Ability of Bacterium Bacillus subtilis to Produce Cytokinins and to Influence the Growth and Endogenous Hormone Content of Lettuce Plants, Plant Soil, 2005, vol. 272, pp. 201–209.CrossRefGoogle Scholar
  6. 6.
    Kudoyarova, G.R., Farhutdinov, R.G., Mitrichenko, A.N., Teplova, I.R., Dedov, A.V., Veselov, S.U., and Kulaeva, O.N., Fast Changes in Growth Rate and Cytokinin Content of the Shoot Following Rapid Cooling of Roots of Wheat Seedling, Plant Growth Regul., 1998, vol. 26, pp. 105–108.CrossRefGoogle Scholar
  7. 7.
    Veselov, S.Y., Kudoyarova, G.R., Egutkin, N.L., Gyuli-Zade, V.G., Mustafina, A.R., and Kof, E.M., Modified Solvent Partitioning Scheme Providing Increased Specificity and Rapidity of Immunoassay for Indole-3-Acetic Acid, Physiol. Plant., 1992, vol. 86, pp. 93–96.CrossRefGoogle Scholar
  8. 8.
    Kudoyarova, G.R., Veselov, S.Yu., Erkeev, M.I., Gyulizade, V.Z., Gimaeva, R.M., Zagidullin, I.V., and Shakirova, F.M., Immunoenzyme Determination of IAA Content in Maize Seeds Using Labeled Antibodies, Fiziol. Rastenii, 1986, vol. 33, pp. 1221–1227.Google Scholar
  9. 9.
    Kudoyarova, G.R., Veselov, S.Yu., Karavaiko, N.N., Gyuli-zade, V.Z., Cheredova, E.P., Mustafina, A.R., Moshkov, I.E., and Kulaeva, O.N., Immunoenzyme Test-System for Cytokinin Determination, Sov. Plant Physiol., 1990, vol. 37, pp. 193–199.Google Scholar
  10. 10.
    Arkhipova, T.N., Veselov, S.Yu., Melent’ev, A.I., Martynenko, E.V., and Kudoyarova, G.R., Effect of Micro-organisms Producing Cytokinins on Plant Growth, Biotekhnologiya, 2006, no. 4, pp. 50–55.Google Scholar
  11. 11.
    Pausheva, Z.P., Praktikum po tsitologii rastenii (Manual for Plant Cytology), Moscow: Agropromizdat, 1988.Google Scholar
  12. 12.
    Del Pozo, J.C., Lopez-Matas, M.A., Ramirez-Parra, E., and Gutierrez, C., Hormonal Control of the Plant Cell Cycle, Physiol. Plant., 2005, vol. 123, pp. 173–183.CrossRefGoogle Scholar
  13. 13.
    Havlová, M., Dobrev, P.I., Motyka, V., Štorchov., Libus, J., Dobrá, J., Malbeck, J., Gaudinová, A., and Vanková, R., The Role of Cytokinins in Responses to Water Deficit in Tobacco Plants Over-Expressing trans-Zeatin O-Glucosyltransferase Gene under 35S or SAG12 Promoters, Plant Cell Environ., 2008, vol. 31, pp. 341–353.PubMedCrossRefGoogle Scholar
  14. 14.
    Hirose, N., Takei, K., Kuroha, T., Kamada-Nobusada, T., Hayashi, H., and Sakakibara, H., Regulation of Cytokinin Biosynthesis, Compartmentalization and Translocation, J. Exp. Bot., 2008, vol. 59, pp. 75–83.PubMedCrossRefGoogle Scholar
  15. 15.
    Banowetz, G.M., Cultivars of Hexaploid Wheat of Contrasting Stature and Chlorophyll Retention Differ in Cytokinin Content and Responsiveness, Ann. Bot., 1997, vol. 79, pp. 185–190.CrossRefGoogle Scholar
  16. 16.
    Dewitte, W., Chiappetta, A., Azmi, A., Witters, E., Strnad, M., Rembur, J., Noin, M., Chriqui, D., and van Onckelen, H., Dynamics of Cytokinins in Apical Shoot Meristems of a Day-Neutral Tobacco during Floral Transition and Flower Formation, Plant Physiol., 1999, vol. 119, pp. 111–121.PubMedCrossRefGoogle Scholar
  17. 17.
    Aloni, R., Langhans, M., Aloni, E., Dreieicher, E., and Ullrich, C.I., Root-Synthesized Cytokinin in Arabidopsis Is Distributed in the Shoot by the Transpiration Stream, J. Exp. Bot., 2005, vol. 56, pp. 1535–1544.PubMedCrossRefGoogle Scholar
  18. 18.
    Hwang, I. and Sakakibara, H., Cytokinin Biosynthesis and Perception, Physiol. Plant., 2006, vol. 126, pp. 528–538.CrossRefGoogle Scholar
  19. 19.
    Veselov, D.S., Veselov, S.Yu., Vysotskaya, L.B., Kudoyarova, G.R., and Farkhutdinov, R.G., Gormony rastenii: regulyatsiya kontsentratsii, svyaz’ s rostom i vodnym obmenom (Plant Hormones: Regulation of the Concentration, Relationships with Growth and Water Relations), Moscow: Nauka, 2007.Google Scholar
  20. 20.
    Polanská, L., Vičánková, A., Nováková, M., Malbeck J., Dobrev, P.I., Brzobohaty, B., Vaňková, R., and Macháčková, I., Altered Cytokinin Metabolism Affects Cytokinin, Auxin, and Abscisic Acid Contents in Leaves and Chloroplasts, and Chloroplast Ultrastructure in Transgenic Tobacco, J. Exp. Bot., 2007, vol. 58, pp. 637–649.PubMedCrossRefGoogle Scholar
  21. 21.
    Hartig, K. and Beck, E., Crosstalk between Auxin, Cytokinins, and Sugars in the Plant Cell Cycle, Plant Biol., 2006, vol. 8, pp. 389–396.PubMedCrossRefGoogle Scholar
  22. 22.
    Skorobogatova, I.V., Zakharova, E.V., Karsunkina, N.P., Kurapov, P.B., Sorokina, G.L., and Kislin, E.N., Changes in the Phytohormones Content in Barley Seedlings during Ontogeny and after the Addition of Growth-Stimulating Regulators, Agrokhimiya, 1999, no. 8, pp. 49–53.Google Scholar
  23. 23.
    Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A., and Fatkhutdinova, D.R., Changes in the Hormonal Status of Wheat Seedlings Induced by Salicylic Acid and Salinity, Plant Sci., 2003, vol. 164, pp. 317–322.CrossRefGoogle Scholar
  24. 24.
    Ghanem, M.E., Albacete, A., Smigocki, A.C., Frebort, I., Pospisilova, H., Martínez-Andujar, C., Acosta, M., Sanchez-Bravo, J., Lutts, S., Dodd, I.C., and Perez-Alfocea, F., Root-Synthesized Cytokinins Improve Shoot Growth and Fruit Yield in Salinized Tomato (Solanum lycopersicum L.) Plants, J. Exp. Bot., 2011, vol. 62, pp. 125–140.PubMedCrossRefGoogle Scholar
  25. 25.
    Dodd, I.C., Hormonal Interactions and Stomatal Responses, J. Plant Growth Regul., 2003, vol. 22, pp. 32–46.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • O. I. Yakhin
    • 1
  • A. A. Lubyanov
    • 1
    • 2
  • I. A. Yakhin
    • 1
  1. 1.Institute of Biochemistry and Genetics, Ufa Research CenterRussian Academy of SciencesUfa, BashkortostanRussia
  2. 2.Orenburg State UniversityUfa Branch, Ufa, BashkortostanRussia

Personalised recommendations