Russian Journal of Plant Physiology

, Volume 59, Issue 5, pp 640–647 | Cite as

Early photosynthetic response of Arabidopsis thaliana to temperature and salt stress conditions

  • A. Martínez-Peñalver
  • E. Graña
  • M. J. Reigosa
  • A. M. Sánchez-Moreiras
Research Papers

Abstract

Temperature changes and salt accumulation are among the most common abiotic factors affecting plants in agricultural and natural ecosystems. The different responses of plants to these factors have been widely investigated in previous works. However, detailed mechanism of the early photosynthetic response (first 24 h) has been poorly studied. The aim of the work was to monitor the early response of adult Arabidopsis thaliana plants exposed to different thermal (cold and heat) and salt conditions. Detailed evaluation of the efficiency of photosystem II was done, and the various routes of energy output as well as measurements of the contents of H2O2, proline, and photosynthetic pigments at different times during the first 24 h of treatment were examined. The conditions used in the study were those that caused a weak stress with time of exposure. Cold-treated plants showed the most continuous inhibitory effect on photosynthetic activity, with a fast metabolic slowdown (reduced PSII efficiency and decreased pigment contents), although they also demonstrated clear acclimation responses (increased heat dissipation and protein content). Heat-treated plants showed a late but stronger effect on photosynthesis with significantly increased quantum yield of nonregulated energy dissipation (ΨNO) and H2O2 content at the last measurements. Finally, salt-induced oxidative stress (increased H2O2 content), decreased PSII efficiency and pigment content.

Keywords

Arabidopsis thaliana chlorophyll a fluorescence stress factors temperature salt 

Abbreviations

DAB

3,3′-diaminobenzidine

PSII

photosystem II

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kreps, J.A., Wu, Y., Chang, H.-S., Zhu, T., Wang, X., and Harper, J.F., Transcriptome Changes for Arabidopsis in Response to Salt, Osmotic, and Cold Stress, Plant Physiol., 2002, vol. 130, pp. 2129–2141.PubMedCrossRefGoogle Scholar
  2. 2.
    Gupta, N.K., Meena, S.K., Gupta, S., and Khandelwal, S.K., Gas Exchange, Membrane Permeability, and Ion Uptake in Two Species of Indian Jujube Differing in Salt Tolerance, Photosynthetica, 2002, vol. 40, pp. 535–539.CrossRefGoogle Scholar
  3. 3.
    Khan, N.A., NaCl Inhibited Chlorophyll Synthesis and Associated Changes in Ethylene Evolution and Antioxidative Enzyme Activities in Wheat, Biol. Plant., 2003, vol. 47, pp. 437–440.CrossRefGoogle Scholar
  4. 4.
    Verslues, P.E., Agarwal, M., Katiyar-Agarwa, S., Zhu, J., and Zhu, J.K., Methods and Concepts in Quantifying Resistance to Drought, Salt and Freezing, Abiotic Stresses That Affect Plant Water Status, Plant J., 2006, vol. 45, pp. 523–539.PubMedCrossRefGoogle Scholar
  5. 5.
    Yeo, A., Review Article, Molecular Biology of Salt Tolerance in the Context of Whole-Plant Physiology, J. Exp. Bot., 1998, vol. 49, pp. 915–929.Google Scholar
  6. 6.
    Andersen, J.K., Oxidative Stress in Neurodegeneration: Cause or Consequence? Nat. Med., 2004, vol. 10, pp. S18–S25.PubMedCrossRefGoogle Scholar
  7. 7.
    Foyer, C.H., Vanacker, H., Gomez, L.D., and Harbinson, J., Regulation of Photosynthesis and Antioxidant Metabolism in Maize Leaves at Optimal and Chilling Temperatures: Review, Plant Physiol. Biochem., 2002, vol. 40, pp. 659–668.CrossRefGoogle Scholar
  8. 8.
    Mahajan, S. and Tuteja, N., Cold, Salinity and Drought Stresses: An Overview, Arch. Biochem. Biophys., 2005, vol. 444, pp. 139–158.PubMedCrossRefGoogle Scholar
  9. 9.
    Allakhverdiev, S.I., Kreslavski, V.D., and Klimov, V.V., Los, D.A., Carpentier, R., and Mohanty, P., Heat Stress: An Overview of Molecular Responses in Photosynthesis, Photosynth. Res., 2008, vol. 98, pp. 541–550.PubMedCrossRefGoogle Scholar
  10. 10.
    Duncan, D.R. and Widholm, J.M., Proline Accumulation and Its Implication in Cold Tolerance of Regenerable Maize Callus, Plant Physiol., 1987, vol. 83, pp. 703–708.PubMedCrossRefGoogle Scholar
  11. 11.
    Lee, T.-M., The Role of Proline Dehydrogenase and Δ-Pyrroline-5-Carboxylate Dehydrogenase in the Regulation of Proline Homeostasis in Gracilaria tenuistipitata (Gigartinales, Rhodophyta) Exposed to High Temperature, Phycologia, 1998, vol. 37, pp. 439–442.CrossRefGoogle Scholar
  12. 12.
    Salvucci, M.E. and Crafts-Brandner, S.J., Inhibition of Photosynthesis by Heat Stress: The Activation State of Rubisco as a Limiting Factor in Photosynthesis, Physiol. Plant., 2004, vol. 120, pp. 179–186.PubMedCrossRefGoogle Scholar
  13. 13.
    Kim, H.J. and Lee, D.H., Long SAGE Analysis of the Early Response to Cold Stress in Arabidopsis Leaf, Planta, 2009, vol. 229, pp. 1181–1200.PubMedCrossRefGoogle Scholar
  14. 14.
    Chaves, M.M., Flexas, J., and Pinheiro, C., Photosynthesis Under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell, Ann. Bot., 2009, vol. 103, pp. 551–560.PubMedCrossRefGoogle Scholar
  15. 15.
    Ramos, P. and Pedrol, N., Free Proline Quantification, Handbook of Plant Ecophysiology Techniques, Reigosa, M.J., Ed., Dordrecht: Kluwer, 2001, pp. 365–382.Google Scholar
  16. 16.
    Pedrol, N. and Ramos, P., Protein Content Quantification by Bradford Method, Handbook of Plant Ecophysiology Techniques, Reigosa, M.J., Ed., Dordrecht: Kluwer, 2001, pp. 283–296.Google Scholar
  17. 17.
    Calatayud, A., Roca, D., and Martinez, P.F., Spatial-Temporal Variations in Rose Leaves under Water Stress Conditions by Imaging Fluorescence, Plant Physiol. Biochem., 2006, vol. 44, pp. 564–573.PubMedCrossRefGoogle Scholar
  18. 18.
    Van Acker, F.A.A., Schouten, O., Haenen, G.R.M., van der Vijgh, W.J.F., and Bast, A., Flavonoids Can Replace Tocopherol as an Antioxidant, FEBS Lett., 2000, vol. 473, pp. 145–148.PubMedCrossRefGoogle Scholar
  19. 19.
    Martínez-Peñalver, A., Reigosa, M.J., and Sánchez-Moreiras, A.M., Imaging Chlorophyll a Fluorescence Reveals Specific Spatial Distributions under Different Stress Conditions, Flora, 2011, vol. 206, pp. 836–844.CrossRefGoogle Scholar
  20. 20.
    Cattivelli, L. and Bartels, D., Cold Induced mRNAs Accumulate with Different Kinetics in Barley Coleoptiles, Planta, 1989, vol. 78, pp. 84–88.Google Scholar
  21. 21.
    Pastori, P., Foyer, C.H., and Mullineaux, P., Low Temperature-Induced Changes in the Distribution of H2O2 and Antioxidants between the Bundle Sheath and Mesophyll Cells of Maize Leaves, J. Exp. Bot., 2000, vol. 51, pp. 107–113.PubMedCrossRefGoogle Scholar
  22. 22.
    Liu, X. and Huang, B., Photosynthetic Acclimation to High Temperatures Associated with Heat Tolerance in Creeping Bentgrass, J. Plant Physiol., 2008, vol. 165, pp. 1947–1953.PubMedCrossRefGoogle Scholar
  23. 23.
    Khedr, A.B.A., Abbas, M.A., Wahid, A.A.A., Quick, W.P., and Abogadallah, G.M., Proline Induces the Expression of Salt-Stress-Responsive Proteins and May Improve the Adaptation of Pancratium maritimum L. to Salt Stress, J. Exp. Bot., 2003, vol. 54, pp. 2553–2562.PubMedCrossRefGoogle Scholar
  24. 24.
    Jaleel, C.A., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A., Sridharan, R., and Panneerselvam, R., Studies on Germination, Seedling Vigour, Lipid Peroxidation and Proline Metabolism in Catharanthus roseus Seedlings under Salt Stress, South Afr. J. Bot., 2007, vol. 73, pp. 190–195.CrossRefGoogle Scholar
  25. 25.
    Verbruggen, N. and Hermans, C., Proline Accumulation in Plants: A Review, Amino Acids, 2008, vol. 35, pp. 753–759.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • A. Martínez-Peñalver
    • 1
  • E. Graña
    • 1
  • M. J. Reigosa
    • 1
  • A. M. Sánchez-Moreiras
    • 1
  1. 1.Department of Plant Biology and Soil Science, Faculty of BiologyUniversity of VigoVigoSpain

Personalised recommendations