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Russian Journal of Plant Physiology

, Volume 58, Issue 4, pp 667–673 | Cite as

Effects of paraquat on lipid peroxidation and antioxidant enzymes in aquatic fern Azolla microphylla

  • A. Sood
  • S. Pabbi
  • P. L. Uniyal
Research Papers

Abstract

Paraquat is most extensively used methyl viologen herbicide to control weeds in the rice-Azolla ecosystem. The effects of different paraquat (PQ) dosages on growth, lipid peroxidation, and activity of antioxidant enzymes of Azolla microphylla Kaul. were investigated. The results indicated that Azolla fronds survived only at the concentrations of 2–6 μM PQ. Frond fragmentation and browning occurred after 24 h at 8 μM PQ. At 24 h, the amount of proteins decreased by 48.7 % in Azolla fronds exposed to 10 μM PQ than that in control fronds. The supplementation of 10 μM PQ increased the activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX) by 2,4-, 1,8-, 3,0-, and 2,2-fold, respectively, as compared with control. The content of PQ and activities of SOD, CAT, GPX, and APX were found to be positively correlated. Our study showed that PQ (2–6 μM) caused ROS overproduction in Azolla fronds, which were scavenged by induced activities of antioxidant enzymes.

Keywords

Azolla paraquat antioxidant enzymes lipid peroxidation reactive oxygen species 

Abbreviations

APX

ascorbate peroxidase

ASA

ascorbate

CAT

catalase

GPX

guaiacol peroxidase

PQ

paraquat

SOD

superoxide dismutase

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.Center for Conservation and Utilization of Blue-Green AlgaeIndian Agricultural Research InstituteNew DelhiIndia

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