Russian Journal of Plant Physiology

, Volume 58, Issue 2, pp 370–374 | Cite as

Effect of pre-sowing γ-irradiation of sea buckthorn seeds on the content and fatty acid composition of total lipids in the seeds of the first plant generation

Brief Communications

Abstract

Absolute content and FA-composition of sea buckthorn (Hippophaë rhamnoides L.) seed lipids were studied. The seeds of cvs. Vitaminnaya and Zyryanka belonging to the Siberian climatype and also the seeds of the first generation (M1) plants grown from the seeds subjected to pre-sowing γ-irradiation (60Co) at the doses of 50 and 100 Gy (cv. Vitaminnaya) and 100, 250, and 500 Gy (cv. Zyryanka) were used in analyses. In all treatments, irradiation resulted in the reduced seed weight in M1 plants, which was sharper in cv. Vitaminnaya. In contrast, oil content declined strongly in cv. Zyryanka seeds, especially after irradiation with 500 Gy, whereas this index remained almost unchanged in cv. Vitaminnaya. Control and treated plants were close by their FA qualitative composition and by the total content of unsaturated FAs (88–90%). Pre-sowing seed irradiation resulted in the rise of the unsaturation index of lipids and linolenic acid concentration; at lower irradiation doses, this rise was more pronounced. With the increase of irradiation dose, the content of linolenic acid reduced in both cultivars, whereas the content of linoleic acid increased. As distinct from cv. Vitaminnaya, in cv. Zyryanka irradiation increased the content of linoleate and reduction in the level of oleate. The conclusion is that pre-sowing γ-irradiation of sea buckthorn seeds could affect substantially on the basic quantitative indices characterizing seeds of M1 plants. The range and direction of induced changes depend on both the dose of irradiation and cultivar genotype.

Keywords

Hippophaäerhamnoides γ-irradiation doses of irradiation plants of the first generation seeds fatty acids linolenic acid 

Abbreviations

Fatty acids: 14:0

myristic

15:0

pentadecanoic

16:0

palmitic

16:1

palmitoleic

17:0

margarinic

18:0

stearic

18:1

oleic

18:2

linoleic

18:3

linolenic

UI

unsaturation index

FAME

methyl ester of FA

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Privalov, G.F., Experimental Mutagenesis and Mutational Selection of Sea Buckthorn, Geneticheskie metody v selektsii rastenii (Genetic Methods in Plant Breeding), Shumny, V.K., Ed., Novosibirsk: Nauka, Sibirskoe Otd., 1992, pp. 210–237.Google Scholar
  2. 2.
    II Mezhdunarodnyi simpozium po oblepikhe (Proc. II Int. Symp. Sea Buckthorn), Novosibirsk: Ross. Akad. S-kh. Nauk, Sibirskoe Otd., 1993.Google Scholar
  3. 3.
    Morgun, V.V., Experimental Mutagenesis and Its Use for Genetic Improvement of Cultivated Plants (Resume of 30-Year Investigations), Fiziol. Biokh. Kul’t. Rast., 1996, vol. 28, pp. 53–72.Google Scholar
  4. 4.
    Zhukov, O.S. and Mokrousova, G.I., New Perspective Sea Buckthorn Species for Central-Black Earth Region, Fiziologiya i biokhimiya kul’turnykh rastenii (Physiology and Biochemistry of Cultivated Plants), Eliseev, I.P., Ed., Gorky: Gork. S-kh. Inst., 1985, pp. 92–96.Google Scholar
  5. 5.
    Zhukov, O.S. and Mokrousova, G.I., Radiation Mutagenesis for Improvement of Biochemical Composition of Sea Buckthorn Fruits, Biologiya, khimiya, introduktsiya i selektsiya oblepikhi (Biology, Chemistry, Introduction, and Breeding of Sea Buckthorn), Eliseev, I.P., Ed., Gorky: Gork. S-kh. Inst., 1986, pp. 67–68.Google Scholar
  6. 6.
    Zhukov, O.S. and Mokrousova, G.I., Generation of Valuable Sea Buckthorn Mutants Using Natural Populations, Mekhanizmy radiatsionnogo mutageneza (Mechanisms of Radiation Mutagenesis), Pushchino, 1989, p. 34.Google Scholar
  7. 7.
    Privalov, G.F., Solonenko, L.P., and Trofimova, O.S., Chemical Composition Variability in Sea Buckthorn Fruits as Applied to Seed Exposure to Ionizing Radiation in Stimulatory Doses, Izv. Sib. Otd. Akad. Nauk SSSR, Ser. Biol., 1971, vol. 2, pp. 72–75.Google Scholar
  8. 8.
    Privalov, G.F. and Solonenko, L.P., Biochemical Composition Variability in Sea Buckthorn M1 Fruits as Applied to Seed Treatment with γ-Radiation in Large Doses, Voprosy teoreticheskoi i prikladnoi genetiki (Theoretic and Applied Genetics), Salganik, R.I., Ed., Novosibirsk, 1976, pp. 72–73.Google Scholar
  9. 9.
    Privalov, G.F. and Solonenko, L.P., Ionizing Radiation as the Factor for Sea Buckthorn Breeding, Genetika, 1977, vol. 13, pp. 2045–2046.Google Scholar
  10. 10.
    Privalov, G.F., Skuridin, G.M., and Solonenko, L.P., Effects of Seed Treatment with Mutagens and Gibberellins on Chemical Composition in Sea Buckthorn M1 Fruits, Biologiya, khimiya i farmakologiya oblepikhi (Sea Buckthorn: Biology, Chemistry, and Pharmacology), Novosibirsk: Nauka, Sibirskoe Otd., 1983, pp. 49–56.Google Scholar
  11. 11.
    Laakso, I., Hiltunen, R., Hovinen, S., Schantz, M., and Huhtikangas, A., Selection of High Linoleic Acid Content in Summer Turnip Rape (Brassica campestris L.): 1. Variation of Fatty Acids in an Irradiated Crossing Material, Acta Agric. Scandinavica, 1982, vol. 32, pp. 397–404.CrossRefGoogle Scholar
  12. 12.
    Green, A.G. and Marshall, D.R., Isolation of Induced Mutants in Linseed (Linum usitatissimum) Having Reduced Linolenic Acid Content, Euphytica, 1984, vol. 33, pp. 321–328.CrossRefGoogle Scholar
  13. 13.
    Ivanov, P. and Ivanov, I.G., Sunflower Mutant Line with the High Content of Palmitic Acid, Genetika i Selektsiya, 1985, vol. 18, pp. 123–127.Google Scholar
  14. 14.
    Encheva, J., Ivanov, P., Tsvetkova, F., and Nikolova, V., Development of a New Initial Breeding Material in Sunflower (Helianthus annuus L.) Using Direct Organogenesis and Somatic Embryogenesis, Euphytica, 1993, vol. 68, pp. 181–185.CrossRefGoogle Scholar
  15. 15.
    Garces, R., Osorio, J., Mancha, M., and Fernandez-Martinez, J.M., Sunflower Mutants with Altered Fatty Acid Composition in the Seed Oil, Plant Lipid Metabolism, Kader, J. C. and Mazliak, P., Eds., Dordrecht: Kluwer, 1995, pp. 512–514.Google Scholar
  16. 16.
    Takagi, Y., Hossain, A.B.M.M., Yanagita, T., Matsueda, T., and Murayama, A., Linolenic Acid Content in Soybean Improved by X-Ray Irradiation, Agric. Biol. Chem., 1990, vol. 54, pp. 1735–1738.Google Scholar
  17. 17.
    Rahman, S.M., Takagi, Y., Kubota, K., Miyamoto, K., and Kawakita, T., High Oleic Acid Mutant in Soybean Induced by X-Ray Irradiation, BioSci. Biotechnol. Biochem., 1994, vol. 58, pp. 1070–1072.CrossRefGoogle Scholar
  18. 18.
    Takagi, Y., Rahman, S.M., Joo, H., and Kawakita, T., Reduced and Elevated Palmitic Acid Mutants in Soybean Developed by X-Ray Irradiation, BioSci. Biotechnol. Biochem., 1995, vol. 59, pp. 1778–1779.CrossRefGoogle Scholar
  19. 19.
    Ozerinina, O.V., Berezhnaya, G.A., and Vereshchagin, A.G., Triacylglycerol Composition and Structure of Sea Buckthorn Fruits Grown in Different Regions, Russ. J. Plant Physiol., 1997, vol. 44, pp. 62–69.Google Scholar
  20. 20.
    Belenko, E.L., Datunashvili, E.N., Tsydendambaev, V.D., and Vereshchagin, A.G., Absolute Content and Extractability of Grape Berry Lipids, Sov. Plant Physiol., 1984, vol. 31, pp. 162–167.Google Scholar
  21. 21.
    Berezhnaya, G.A., Eliseev, I.P., Tsydendambaev, V.D., and Vereshchagin, A.G., Determination of Fatty Acid Composition and Content of Acylated Lipids in Sea Buckthorn Fruits, Prikl. Biokhim. Mikrobiol., 1988, vol. 24, pp. 568–572.Google Scholar
  22. 22.
    Berezhnaya, G.A., Ozerinina, O.V., Eliseev, I.P., Tsydendambaev, V.D., and Vereshchagin, A.G., Dynamics of Absolute Content and Fatty Acid Composition of Acylated Lipids in Sea Buckthorn Maturing Fruits, Sov. Plant Physiol., 1992, vol. 39, pp. 1187–1196.Google Scholar
  23. 23.
    Korobova, N.A., Kotova, G.P., and Popesku, O.V., Comparative Efficiency of Chemical and Physical Mutagens Action on Growth and Development of M1 Maize Plants, Primenenie fizicheskikh i khimicheskikh mutagennykh faktorov v selektsii i genetike polevykh kul’tur (Use of Physical and Chemical Mutagenic Factors in Crop Breeding and Genetics), Unguryan, V.G., Ed., Chisinau, 1985, pp. 58–63.Google Scholar
  24. 24.
    Vereshchagin, A.G. and Ganieva, M., Lipid Metabolism in Maturing and Germinating Cotton Seeds and Effect of γ-Radiation on This Process, Biokhimiya, 1964, vol. 29, pp. 288–299.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

Personalised recommendations