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Effect of wounding on gene expression involved in artemisinin biosynthesis and artemisinin production in Artemisia annua

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Abstract

Abstract-Effects of mechanical wounding on gene expression involved in artemisinin biosynthesis and artemisinin production in Artemisia annua leaves were investigated. HPLC-ELSD analysis indicated that there was a remarkable enhancement of the artemisinin content in 2 h after wounding treatment, and the content reached the maximum value at 4 h (nearly 50% higher than that in the control plants). The expression profile analysis showed that many important genes (HMGR, ADS, CPR, and CYP71AV1) involved in the artemisinin biosynthetic pathway were induced in a short time after wounding treatment. This study indicates that the artemisinin biosynthesis is affected by mechanical wounding. The possible mechanism of the control of gene expression during wounding is discussed.

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Abbreviations

HPLC-ELSD:

high performance liquid chromatography-evaporative light scattering detection

MeJa:

methyl jasmonate

MS:

Murashige and Skoog nutrient medium

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Authors and Affiliations

  1. Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China

    Donghui Liu, Lida Zhang, Chengxiang Li, Ke Yang, Yueyue Wang & Kexuan Tang

  2. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, P. R. China

    Xiaofen Sun & Kexuan Tang

  3. Department of Biochemistry and Molecular Biology, Shenyang Medical College, Shenyang, Liaoning, 110034, P. R. China

    Donghui Liu

Authors
  1. Donghui Liu
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  2. Lida Zhang
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  3. Chengxiang Li
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  4. Ke Yang
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  5. Yueyue Wang
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  6. Xiaofen Sun
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  7. Kexuan Tang
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Correspondence to Kexuan Tang.

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Liu, D., Zhang, L., Li, C. et al. Effect of wounding on gene expression involved in artemisinin biosynthesis and artemisinin production in Artemisia annua . Russ J Plant Physiol 57, 882–886 (2010). https://doi.org/10.1134/S102144371006018X

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  • DOI: https://doi.org/10.1134/S102144371006018X

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