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Russian Journal of Plant Physiology

, Volume 57, Issue 5, pp 648–655 | Cite as

Effect of root-applied spermidine on growth and respiratory metabolism in roots of cucumber (Cucumis sativus) seedlings under hypoxia

  • Y. X. Jia
  • J. Sun
  • S. R. GuoEmail author
  • J. Li
  • X. H. Hu
  • S. P. Wang
Research Papers

Abstract

The effects of exogenous spermidine (Spd) application to hypoxic nutrient solution on the contents of endogenous polyamines (PAs) and respiratory metabolism in the roots of cucumber (Cucumis sativus L.) seedlings were investigated. Cucumber seedlings were grown hydroponically in control and hypoxic nutrient solutions with and without addition of Spd at a concentration of 0.05 mM. The activities of key enzymes involved in the tricarboxylic acid cycle (TCAC), such as succinate dehydrogenase (SDH) and isocitrate dehydrogenase (IDH), were significantly inhibited under root-zone hypoxia with dissolved oxygen (DO) at 1 mg/l. In contrast, the activities of enzymes involved in the process of fermentation, such as pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), and alanine aminotransferase (AlaAT), were significantly increased. Thus, aerobic respiration was inhibited and fermentation was enhanced in the roots of cucumber seedlings as a result of decreasing ATP content to inhibit the dry weight of seedlings under hypoxic stress. Moreover, the contents of free, soluble conjugated, and insoluble bound putrescine (Put), Spd, and spermine (Spm) in the roots of cucumber seedlings were significantly increased under hypoxia stress. Interestingly, application of Spd to hypoxic roots markedly suppressed the accumulation of free Put and, in contrast, promoted an increase in free Spd and Spm, as well as soluble conjugated and insoluble bound Put, Spd, and Spm contents. From these data, we deduced that exogenous Spd promotes the conversion of free Put into free Spd and Spm, and soluble conjugated and insoluble bound PAs under hypoxia stress. Furthermore, the activities of LDH, PDC, and ADH were suppressed and, in contrast, the activities of SDH and IDH were enhanced by application of exogenous Spd to hypoxic roots. As a result, aerobic respiration was enhanced but fermentation metabolism was inhibited in the roots of cucumber seedlings, leading to an increase in ATP content to alleviate the inhibited dry weight of seedlings due to hypoxia stress. These results suggest that application of Spd to hypoxic nutrient solution promoted conversion of free Put into free Spd and Spm as well as soluble conjugated and insoluble bound PAs, further enhanced IDH and SDH activities, and inhibited ethanol fermentation and lactate fermentation, resulting in increased ATP content and eventually enhanced tolerance of cucumber plants to root-zone hypoxia.

Key words

Cucumis sativus spermidine hypoxia growth polyamines 

Abbreviations

AlaAT

alanine aminotransferase

ADH

alcohol dehydrogenase

DO

dissolved oxygen

IDH

isocitrate dehydrogenase

LDH

lactate dehydrogenase

PAs

polyamines

Put

putrescine

PDC

pyruvate decarboxylase

Spd

spermidine

Spm

spermine

SDH

succinate dehydrogenase

TCAC

tricarboxylic acid cycle

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Y. X. Jia
    • 1
    • 2
  • J. Sun
    • 1
  • S. R. Guo
    • 1
    Email author
  • J. Li
    • 1
  • X. H. Hu
    • 1
  • S. P. Wang
    • 1
  1. 1.Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture; College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.College of Resources and EnvironmentSichuan Agricultural UniversityYaanChina

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