Rapid evolution of promoters for the plastome gene ndhF in flowering plants
- 113 Downloads
Plastome is thought to be a very conservative part of plant genome but little is known about the evolution of plastome promoters. It was previously shown that one light-regulated promoter (LRPpsbD) is highly conserved in different flowering plant species and in black pine. We have undertaken search and demonstrated that gene ndhF is located in a plastome region that rarely underwent substantial rearrangements in terrestrial plants. However, alignment of sequences upstream ndhF suggests that promoters of this gene underwent comparatively rapid evolution in flowering plants. Probably, the ancestor of two basal Magnoliophyta branches (magnoliids and eudicotyledons) had the promoter PA-ndhF, which was substituted with other promoters—PB-ndhF and PC-ndhF—in some phylogenetic lineages of dicots. We failed to reveal conservative sequences with potential promoters of −10/−35 type upstream ndhF genes of monocotyledonous plants, including nine representatives of the grass family (Poaceae). Multiple alignments of sequences from related taxa showed that the predicted ndhF promoters (A–C) underwent frequent mutations and these mutations are not only nucleotide substitutions but also small insertions and deletions. Thus, we can assume that at least some plastome promoters evolve rapidly.
Key wordsflowering plants plastome promoter evolution ndhF
insertion or deletion
light-regulated promoter of psbD gene
nucleus-encoded plastid RNA polymerase
plastid-encoded RNA polymerase
small single-copy region (in plastome).
Unable to display preview. Download preview PDF.
- 2.Turmel, M., Otis, C., and Lemieux, C., The Chloroplast and Mitochondrial Genome Sequences of the Charophyte Chaetosphaeridium globosum: Insights into the Timing of the Events That Restructured Organelle DNAs within the Green Algal Lineage That Led to Land Plants, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 11275–11280.CrossRefPubMedGoogle Scholar
- 3.Palmer, J.D., Comparison of Chloroplast and Mitochondrial Genome Evolution in Plants, Cell Organelles, Herrmann, R.G., Ed., Wien: Springer-Verlag, 1992, pp. 99–136.Google Scholar
- 18.Greiner, S., Wang, X., Rauwolf, U., Silber, M.V., Mayer, K., Meurer, J., Haberer, G., and Herrmann, R.G., The Complete Nucleotide Sequences of the Five Genetically Distinct Plastid Genomes of Oenothera, Subsection Oenotera: I. Sequence Evaluation and Plastome Evolution, Nucleic Acids Res., 2008, vol. 36, pp. 2366–2378.CrossRefPubMedGoogle Scholar
- 22.Kim, M., Thum, K.E., Morishige, D.T., and Mullet, J.E., Detailed Architecture of the Barley Chloroplast psbD-psbC Blue Light-Responsive Promoter, J. Biochem., 1999, vol. 274, pp. 4684–4692.Google Scholar