Expression of WRKY transcription factor and stress protein genes in wheat plants during cold hardening and ABA treatment
Changes in expression of WRKY transcription factor and stress protein genes (Wcor15, Wrab17, Wrab19, and Wcs120) were studied on wheat (Triticum aestivum L., cv. Moskovskaya 39) seedlings exposed to cold hardening for 7 days at 4°C. The high level of WRKY gene expression was noticed already in 15 min after the beginning of cold treatment, but the expression level lowered during longer treatments. Exogenous ABA (0.1 mM) suppressed the WRKY gene expression. The level of Wcor15 gene expression increased gradually, reaching the peak on the second day, and then decreased. Gene expression of Wrab17 remained elevated throughout the period of cold exposure (7 days), and expression of Wrab19 was promoted within the first two days. Exogenous ABA induced expression of Wcor15, Wrab17, and Wrab19 genes both at cold-hardening (4°C) and normal (22°C) temperatures. A significant increase in Wcs120 gene expression during cold hardening was ABA-independent. It is concluded that the increase of wheat plant resistance at the initial stage of cold hardening is related to gene expression of WRKY transcription factor and of stress proteins (Wcor15, Wrab17, Wrab19, and Wcs120), while the resistance increase during prolonged adaptation is related to gene expression of Wcor15 and Wrab17.
Key wordsTriticum aestivum cold hardening gene expression WRKY transcription factor Cor-genes ABA
the median lethal temperature that kills 50% of parenchymal leaf cells.
Unable to display preview. Download preview PDF.
- 4.Trunova, T.I., Rastenie i nizkotemperaturnyi stress (Plant and Low-Temperature Stress), Moscow: Nauka, 2007.Google Scholar
- 12.Drozdov, S.N., Kurets, V.K., Budykina, N.P., and Balagurova, N.I., Determination of Plant Frost-Resistance, Metody otsenki ustoichivosti rastenii k neblagopriyatnym usloviyam sredy (Methods for the assessment of Plant Tolerance to Unfavorable Environments), Leningrad: Vses. Inst. Rastenievod., 1976, pp. 222–228.Google Scholar
- 22.Gavrikova, O.M., Relationships between Protein Content and Processing Characteristics of the Grain of Soft Winter Wheat Varieties, Cand. Sci. (Biol.) Dissertation, Moscow: Rossiiskii agrarnyi universitet-Mosk. s-kh. akad., 2007.Google Scholar
- 26.Kume, S., Kobayashi, F., Ishibashi, M., Ohno, R., Nakamura, C., and Takumi, S., Differential and Coordinated Expression of Cbf and Cor/Lea Genes during Long-Term Cold Acclimation in Two Wheat Cultivars Showing Distinct Levels of Freezing Tolerance, Gen. Genet. Syst., 2005, vol. 80, pp. 185–197.CrossRefGoogle Scholar
- 28.Xiong, L., Schumaker, K.S., and Zhu, J.-K., Cell Signaling during Cold, Drought, and Salt Stress, Plant Cell, 2002, Suppl. Iss., pp. 165–183.Google Scholar