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Photochemical heat-shock response in common bean leaves as affected by previous water deficit

  • R. V. Ribeiro
  • M. G. Santos
  • E. C. Machado
  • R. F. Oliveira
Research Papers

Abstract

The heat sensitivity of photochemical processes was evaluated in the common bean (Phaseolus vulgaris) cultivars A222, A320, and Carioca grown under well-watered conditions during the entire plant cycle (control treatment) or subjected to a temporal moderate water deficit at the preflowering stage (PWD). The responses of chlorophyll fluorescence to temperature were evaluated in leaf discs excised from control and PWD plants seven days after the complete recovery of plant shoot hydration. Heat treatment was done in the dark (5 min) at the ambient CO2 concentration. Chlorophyll fluorescence was assessed under both dark and light conditions at 25, 35, and 45°C. In the dark, a decline of the potential quantum efficiency of photosystem II (PSII) and an increase in minimum chlorophyll fluorescence were observed in all genotypes at 45°C, but these responses were affected by PWD. In the light, the apparent electron transport rate and the effective quantum efficiency of PSII were reduced by heat stress (45°C), but no change due to PWD was demonstrated. Interestingly, only the A222 cultivar subjected to PWD showed a significant increase in nonphotochemical fluorescence quenching at 45°C. The common bean cultivars had different photochemical sensitivities to heat stress altered by a previous water deficit period. Increased thermal tolerance due to PWD was genotype-dependent and associated with an increase in potential quantum efficiency of PSII at high temperature. Under such conditions, the genotype responsive to PWD treatment enhanced its protective capacity against excessive light energy via increased nonphotochemical quenching.

Key words

Phaseolus vulgaris chlorophyll fluorescence photoprotection photosynthesis temperature water deficit 

Abbreviations

ETR

apparent electron transport rate

FM

maximum chlorophyll fluorescence yield under dark conditions

F′M

maximum chlorophyll fluorescence yield under light conditions

F0

minimum chlorophyll fluorescence yield under dark conditions

FS

steady-state chlorophyll fluorescence yield under light conditions

FV

variable chlorophyll fluorescence under dark conditions

FV/FM

potential quantum efficiency of PSII

LHCII

light-harvesting chlorophyll a/b protein complexes of PSII

NPQ

nonphotochemical quenching of chlorophyll fluorescence

Q

photosynthetic photon flux density

PS

photosystem

PWD

previous moderate water deficit

QA

primary plastoquinone acceptor of PSII

QB

secondary plastoquinone acceptor of PSII

ΔF

variable chlorophyll fluorescence under light conditions

ΔF/FM

effective quantum efficiency of PSII

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • R. V. Ribeiro
    • 1
  • M. G. Santos
    • 2
  • E. C. Machado
    • 1
  • R. F. Oliveira
    • 3
  1. 1.Section of Plant Physiology, Center for Research and Development in Ecophysiology and BiophysicsAgronomic InstituteCampinasBrazil
  2. 2.Department of BotanyFederal University of PernambucoRecifieBrazil
  3. 3.Department of Biological Sciences, ‘Luiz de Queiroz’ College of AgricultureUniversity of Sao PauloPiracicabaBrazil

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