Russian Journal of Plant Physiology

, Volume 54, Issue 5, pp 676–682

Physiological responses of wheat seedlings to drought and UV-B radiation. Effect of exogenous sodium nitroprusside application

Research Papers

Abstract

Physiological and biochemical responses of wheat seedlings to drought, UV-B radiation, and combined stress were investigated. Drought, UV-B, and combined stresses retarded seedling growth by 26.5, 29.1, and 55.9%, respectively. One reason for growth retardation may be the oxidative damage indicated by an increase in the H2O2 content and lipid peroxidation degree. Furthermore, there was negative correlation between shoot fresh weight and H2O2 content, fresh weight and the content of thiobarbituric acid-reacting substances (TBARS), and the positive correlation between H2O2 content and TBARS (R2 = 0.9251, 0.9005, and 0.9007, respectively). The activities of superoxide dismutase, guaiacol peroxidase, and ascorbate peroxidase increased under drought, UV-B, and the combination of stresses, while catalase activity decreased under the combined stress as compared to the control. The combination of drought and UV-B caused more severe damage to wheat seedlings than stress factors applied separately. Thus, the combined application of drought and UV-B had more strong adverse effects on wheat seedlings. The addition of 0.2 mM sodium nitroprusside (SNP) enhanced wheat seedling growth under drought, UV-B, and combined stress, likely, due to decreasing the accumulation of H2O2 and lipid peroxidation as well as activating the antioxidant enzymes. However, SNP treatment decreased the proline content.

Key words

Triticum aestivum antioxidant enzymes drought stress nitric oxide proline accumulation UV-B irradiation 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

GPX

guaiacol peroxidase

ROS

reactive oxygen species

SNP

sodium nitroprusside

SOD

superoxide dimutase

TBA

thiobarbituric acid

TBARS

TBA-reacting substances

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References

  1. 1.
    Balakumar, T., Hani, B.V.V., and Paliwal, K., On the Interaction of UV-B Radiation (280–315 nm) with Water Stress in Crop Plants, Physiol. Plant., 1993, vol. 87, pp. 217–222.CrossRefGoogle Scholar
  2. 2.
    Sullivan, J.H. and Teramura, A.H., Field Study of the Interaction between Solar Ultraviolet-B Radiation and Drought on Photosynthesis and Growth in Soybean, Plant Physiol., 1990, vol. 92, pp. 141–146.PubMedGoogle Scholar
  3. 3.
    Tevini, M., Iwanzik, W., and Teramura, A.H., Effects of UV-B Radiation on Plants during Mild Water Stress, Z. Pflanzenphysiologie, 1983, vol. 110, pp. 459–467.Google Scholar
  4. 4.
    Teramura, A.H., Foresth, I.N., and Lydon, J., Effects of UV-B Radiation on the Plants during Mild Water Stress: 4. The Insensitivity of Soybean Internal Water Relations to UV-B Radiation, Physiol. Plant., 1984, vol. 62, pp. 384–389.CrossRefGoogle Scholar
  5. 5.
    Teramura, A.H., Sullivan, J.H., and Lydon, J., Effects of UV-B Radiation on Soybean Yield and Seed Quality: A 6-Year Field Study, Physiol. Plant., 1990, vol. 80, pp. 5–11.CrossRefGoogle Scholar
  6. 6.
    Uchida, A., Jagendorf, A.T., Hibino, T., and Takabe, T., Effects of Hydrogen Peroxide and Nitric Oxide on Both Salt and Heat Stress Tolerance in Rice, Plant Sci., 2002, vol. 163, pp. 515–523.CrossRefGoogle Scholar
  7. 7.
    Tu, J., Shen, W.B., and Xu, L.L., Regulation of Nitric Oxide on the Aging Process of Wheat Leaves, Acta Bot. Sinica, 2003, vol. 45, pp. 1055–1062.Google Scholar
  8. 8.
    Beligni, M.V. and Lamattina, L., Nitric Oxide Counteracts Cytotoxic Processes Mediated by Reactive Oxygen Species in Plant Tissues, Planta, 1999, vol. 208, pp. 337–344.CrossRefGoogle Scholar
  9. 9.
    Frank, S. and Podda, M., Identification of Copper/Zinc Superoxide Dismutase as a Nitric Oxide-Regulated Gene in Human (HaCaT) Keratinocytes: Implications for Keratinocyte Proliferation, Biochem. J., 2000, vol. 346, pp. 719–728.PubMedCrossRefGoogle Scholar
  10. 10.
    Caldwell, M.M., Solar UV-B Irradiation and the Growth and Development of Higher Plant, Photophysiology, vol. 6, Giese, A.C., Ed., New York: Academic, 1971, pp. 131–171.Google Scholar
  11. 11.
    Brennan, T. and Frenkel, C., Involvement of Hydrogen Peroxide in the Regulation of Senescence in Pear, Plant Physiol., 1977, vol. 59, pp. 411–416.PubMedGoogle Scholar
  12. 12.
    Hodges, D.M., DeLong, J.M., Forney, C.F., and Prange, R.K., Improving the Thiobarbituric Acid-Reactive-Substance Assay for Estimating Lipid Peroxidation in Plant Tissues Containing Anthocyanin and Other Interfering Compounds, Planta, 1999, vol. 207, pp. 604–611.CrossRefGoogle Scholar
  13. 13.
    Bates, L.S., Waldren, S.P., and Teare, I.D., Rapid Determination of Free Proline for Water-Stress Studies, Plant Soil, 1973, vol. 39, pp. 205–207.CrossRefGoogle Scholar
  14. 14.
    Giannopotitis, C.N. and Ries, S.K., Superoxide Dismutase in Higher Plants, Plant Physiol., 1977, vol. 59, pp. 309–314.Google Scholar
  15. 15.
    Egley, G.H., Paul, R.N., Vaughn, K.C., and Duke, S.O., Role of Peroxidase in the Development of Water Impermeable Seed Coats in Sida spinosa L., Planta, 1983, vol. 157, pp. 224–232.CrossRefGoogle Scholar
  16. 16.
    Nakano, Y. and Asada, K., Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.Google Scholar
  17. 17.
    Cakmak, I. and Marschner, H., Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean Leaves, Plant Physiol., 1992, vol. 98, pp. 1222–1227.PubMedCrossRefGoogle Scholar
  18. 18.
    Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.PubMedCrossRefGoogle Scholar
  19. 19.
    Smirnoff, N., Plant Resistance to Environmental Stress, Curr. Opin. Biotechnol., 1998, vol. 9, pp. 214–219.PubMedCrossRefGoogle Scholar
  20. 20.
    Alexieva, V., Ivanov, S., Sergiev, I., and Karanov, E., Interaction between Stresses, Bulg. J. Plant Physiol., 2003, spec. iss., pp. 1–7.Google Scholar
  21. 21.
    Alexieva, V., Sergiev, I., Mapelli, S., and Karanov, E., The Effect of Drought and Ultraviolet Radiation on Growth and Stress Markers in Pea and Wheat, Plant, Cell Environ., 2001, vol. 24, pp. 1337–1344.CrossRefGoogle Scholar
  22. 22.
    Rout, N.P. and Shaw, B.P., Salt Tolerance in Aquatic Macrophytes: Possible Involvement of the Antioxidative Enzymes, Plant Sci., 2001, vol. 160, pp. 415–423.PubMedCrossRefGoogle Scholar
  23. 23.
    Reddy, A.R., Chaitanya, K.V., and Vivekanandan, M., Drought-Induced Responses of Photosynthesis and Antioxidant Metabolism in Higher Plants, J. Plant Physiol., 2004, vol. 161, pp. 1189–1202.CrossRefGoogle Scholar
  24. 24.
    Sgherri, C.L. and Navari-Izzo, F., Sunflower Seedlings Subjected to Water Deficit Stress: Oxidative Stress and Defense Mechanisms, Physiol. Plant., 1995, vol. 93, pp. 25–30.CrossRefGoogle Scholar
  25. 25.
    Zhang, C.S., Lu, Q., and Verma, D.P.S., Removal of Feedback Inhibition of P5CS, a Bifunctional Enzyme Catalyzing the First Two Steps of Proline Biosynthesis in Plants, J. Biol. Chem., 1995, vol. 270, pp. 20491–20496.PubMedCrossRefGoogle Scholar
  26. 26.
    Ruiz, J.M., Sanchez, E., Garcia, P., Lopez-Lefebre, L.R., Rivero, R.M., and Romero, L., Proline Metabolism and NAD Kinase Activity in Green Bean Plants Subjected to Cold-Shock, Phytochemistry, 2002, vol. 59, pp. 473–478.PubMedCrossRefGoogle Scholar
  27. 27.
    Chen, C.T., Chen, L.M., Lin, C.C., and Kao, C.H., Regulation of Proline Accumulation in Detached Rice Leaves Exposed to Excess Copper, Plant Sci., 2001, vol. 160, pp. 283–290.PubMedCrossRefGoogle Scholar
  28. 28.
    Carletti, P., Masi, A., Wonisch, A., Grill, D., Tausz, M., and Ferretti, M., Changes in Antioxidant and Pigment Pool Dimensions in UV-B Irradiated Maize Seedlings, Environ. Exp. Bot., 2003, vol. 50, pp. 149–157.CrossRefGoogle Scholar
  29. 29.
    Conner, E.M. and Grisham, M.B., Inflammation, Free Radicals and Antioxidants, Nutrition, 1996, vol. 12, pp. 274–277.PubMedCrossRefGoogle Scholar
  30. 30.
    Lamotte, O., Gould, K., Lecourieux, D., Sequeira-Legrand, A., Lebun-Garcia, A., Durner, J., Pugin, A., and Wendehenne, D., Analysis of Nitric Oxide Signaling Functions in Tobacco Cells Challenged by the Elicitor Cryptogein, Plant Physiol., 2004, vol. 135, pp. 516–529.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  1. 1.School of Biological Resources and Environmental SciencesJishou UniversityHunanChina
  2. 2.Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaChina

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