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Russian Journal of Plant Physiology

, Volume 54, Issue 2, pp 202–206 | Cite as

Cloning, expression, and antitumor activity of recombinant protein of curcin

  • M. J. Luo
  • W. X. Liu
  • X. Y. Yang
  • Y. Xu
  • F. Yan
  • P. Huang
  • F. Chen
Research Papers

Abstract

Curcin, a protein isolated from the seeds of Jatropha curcas can be used as a cell-killing agent. To elaborate the purification methods and investigate the antitumor activity of the recombinant protein, the fragment encoding the mature protein of curcin was inserted into E. coli strain M15 and the recombinant strain was induced to express by the optimum inducer (0.5 mM isopropyl-β-D-thiogalactopyranoside). The recombinant protein was expressed in the form of the inclusion body and was purified by Ni-NTA affinity chromatography. The protein of interest was incubated with the tumor cells at various concentrations for different time. It was shown that the target protein could inhibit the growth of NCL-H446, SGC-7901, and S180 at a very low concentration.

Key words

Jatropha curcas Esherichia coli curcin expression purification recombinant protein antitumor activity 

Abbreviations

Gn

guanidine chloride

IPTG

isopropyl-β-D-thiogalactopyranoside

PBS

phosphate-buffered saline

PMSF

phenylmethylsulfonyl fluoride

RIP

ribosome-inactivating protein

RT-PCR

reverse transcription-polymerase chain reaction.

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Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • M. J. Luo
    • 1
  • W. X. Liu
    • 2
  • X. Y. Yang
    • 1
  • Y. Xu
    • 1
  • F. Yan
    • 1
  • P. Huang
    • 2
  • F. Chen
    • 1
  1. 1.College of Life and ScienceSichuan University 24ChengduChina
  2. 2.Chengdu Institute for Family PlanningChengduChina

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