Russian Journal of Plant Physiology

, Volume 53, Issue 4, pp 469–473

Specific features of oxidative stress in the chilled tobacco plants following transformation with the desC gene for acyl-lipid Δ9-desaturase from Synechococcus vulcanus

  • V. N. Popov
  • N. V. Kipaikina
  • N. V. Astakhova
  • T. I. Trunova
Article

Abstract

Tobacco plants (Nicotiana tabacum L.) transformed with the desC gene for acyl-lipid Δ9-desaturase from a thermophilic cyanobacterium Synechococcus vulcanus were cultivated on the agarized Murashige and Skoog medium at 22°C and a 16-h photoperiod. Tobacco plants transformed with an empty binary vector pGA482 served as the control. The investigations showed that, in contrast to the control, transgenic plants maintained a higher activity of antioxidant enzymes during 2-h incubation at 2°C; as a result, these plants resisted more efficiently the accumulation of reactive oxygen species and reduced the rate of the lipid peroxidation. The activity of antioxidant enzymes in the transformed plants is apparently related to the operation of the introduced desC gene for acyl-lipid Δ9-desaturase because the enhanced activity of the latter increased the relative content of polyunsaturated FAs in membrane lipids and in this way promoted the liquid state of membranes during the chilling period. These changes helped preserve the cellular homeostasis and thereby maintain the steady synthesis of antioxidant enzymes at hypothermic conditions; as a result, cold resistance of transformed tobacco plants increased.

Key words

Nicotiana tabacum transgenic plants acyl-lipid desaturase antioxidant enzymes cold resistance 

Abbreviations

FA

fatty acid

MDA

malondialdehyde

POL

peroxidation of lipids

ROS

reactive oxygen species

SOD

superoxide dismutase.

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Copyright information

© MAIK “Nauka/Interperiodica” 2006

Authors and Affiliations

  • V. N. Popov
    • 1
  • N. V. Kipaikina
    • 1
  • N. V. Astakhova
    • 1
  • T. I. Trunova
    • 1
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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