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Russian Journal of Plant Physiology

, Volume 53, Issue 4, pp 456–462 | Cite as

Peptide conjugates of benzene and toluene metabolites in English ryegrass

  • D. I. Chrikishvili
  • G. V. Zaalishvili
  • T. I. Mitaishvili
  • E. P. Lomidze
Article
  • 81 Downloads

Abstract

Biotransformation of [1-6-14C]benzene and [1-14C]toluene in English ryegrass (Lolium perenne L.) seedlings was investigated. Vapors of these compounds were absorbed by the leaves of this plant. Benzene and toluene were oxidized, forming phenol and benzoic acid, respectively. A portion of phenol and benzoic acid was bound by low-molecular-weight peptides forming conjugates. A qualitative amino acid composition of the peptides involved in the conjugation was determined. After removing plants from the atmosphere containing [1-6-14C]benzene and [1-14C]toluene, the radioactivity of the conjugates gradually decreased. This process was accompanied by the evolution of 14CO2, indicating the breakdown of these conjugates. Radioactive compounds thus formed were oxidized, yielding carbon dioxide. A portion of phenol and benzoic acid, along with peptide conjugation, was subjected to further oxidative transformations up to disruption of the aromatic ring. By this pathway, nonvolatile carboxylic acids, such as muconic, fumaric, succinic, malic, malonic, glycolic, and glyoxylic, were formed. Using electron microscopy, a damaging effect of benzene on the cell ultrastructure of English ryegrass leaves was shown, and this toxic effect depended on the benzene concentration.

Key words

Lolium perenne benzene toluene peptide conjugation xenobiotic ultrastructure 

Abbreviations

IR

infrared

Tm

melting temperature

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Copyright information

© MAIK “Nauka/Interperiodica” 2006

Authors and Affiliations

  • D. I. Chrikishvili
    • 1
  • G. V. Zaalishvili
    • 1
  • T. I. Mitaishvili
    • 2
  • E. P. Lomidze
    • 1
  1. 1.Durmishidze Institute of Biochemistry and BiotechnologyAcademy of Sciences of GeorgiaTbilisiGeorgia
  2. 2.State Agricultural University of GeorgiaTbilisiGeorgia

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