Advertisement

Russian Journal of Plant Physiology

, Volume 53, Issue 3, pp 289–297 | Cite as

Transients of delayed fluorescence induction signal and photosynthetic antennas: A possible relationship. Mathematical modeling approach

  • A. Kalauzi
  • D. Z. Marković
  • Č. N. Radenović
Article

Abstract

A mathematical model was developed for resolved temporal transients of experimentally recorded delayed fluorescence (DF) induction signal. During an intermittent light regime, antennas of the photosynthetic apparatus were treated as targets, repeatedly hit by potentially absorbable photons within a series of consecutive light flashes. Formulas were derived for the number of antennas, cumulatively hit by a specific number of photons, as a function of the flash serial number (time). Model parameters included number of absorbable photons in one flash, antenna sizes, and their number. A series of induction curves were analyzed, obtained from a Zea mays leaf segment and differing in the previous dark period (t d). Each curve, consisting of the two most prominent DF transients (C and D), was fitted with several model types, differing in the number of absorbed photons. For both transients, the best fitting result was achieved when DF induction was linked to the second absorbed photon. As expected, model parameters related to antenna sizes showed weaker dependence on t d than those referring to antenna number. With restrictions applied to this model, the two DF induction transients may be related to two classes of photosynthetic antennas. Their different sizes may have a predominant influence on the efficiency of photon absorption and possibly time-dependent appearance of DF transients.

Key words

Zea mays delayed fluorescence induction transients mathematical modeling photosynthetic antennas 

Abbreviations

Chl

chlorophyll

DF

delayed fluorescence

ECG

electrochemical gradient

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lang, M. and Lichtenthaler, H.K., Changes in the Blue-Green and Red Fluorescence Emission Spectra of Beech Leaves during the Autumnal Chlorophyll Breakdown, J. Plant Physiol., 1991, vol. 138, pp. 550–553.Google Scholar
  2. 2.
    Krause, G.H. and Weis, E., Chlorophyll Fluorescence and Photosynthesis: The Basics, Annu. Rev. Plant Physiol. Plant. Mol. Biol., 1991, vol. 42, pp. 313–349.CrossRefGoogle Scholar
  3. 3.
    Govindjee, van der Ven, M., Preston, C., Seibert, M., and Gratton, E., Chlorophyll a Fluorescence Lifetime Distribution in Open and Closed Photosystem II Reaction Centre Preparation, Biochim. Biophys. Acta, 1990, vol. 1015, pp. 173–179.PubMedCrossRefGoogle Scholar
  4. 4.
    Schmuck, G., Moya, I., Pedrini, A., van der Linde, D., Lichtenthaler, H.K., Stober, F., Schindler, C., and Goulas, Y., Chlorophyll Fluorescence Lifetime Determination of Water Stressed C3 and C4 Plants, Rad. Environ. Biophys., 1992, vol. 31, pp. 141–151.CrossRefGoogle Scholar
  5. 5.
    Sonneveld, A., Duysens, N.M.L., and Moerdijk, A., Submicrosecond Chlorophyll Delayed Fluorescence from Photosystem I. Magnetic Field Induced Increase of the Emission Yield, Biochim. Biophys. Acta, 1981, vol. 636, pp. 39–49.PubMedCrossRefGoogle Scholar
  6. 6.
    Mimuro, M., Akimoto, S., Yamazaki, I., Miyashita, H., and Miyachi, S., Fluorescence Properties of Chlorophyll d-Dominating Prokaryotic Alga, Acaryochloris marina: Studies Using Time-Resolved Fluorescence Spectroscopy on Intact Cells, Biochim. Biophys. Acta, 1999, vol. 1412, pp. 37–46.PubMedCrossRefGoogle Scholar
  7. 7.
    Haveman, J. and Lavorel, J., Identification of the 120 msec Phase in the Decay of Delayed Fluorescence in Spinach Chloroplasts and Subchloroplast Particles as the Intrinsic Back Reaction. The Dependence of the Level of This Phase on the Thylakoids Internal pH, Biochim. Biophys. Acta, 1975, vol. 408, pp. 269–283.PubMedCrossRefGoogle Scholar
  8. 8.
    Holzapfel, C. and Haug, A., Time Course of Microsecond Delayed Light Emission from Scenedesmus obliquus, Biochim. Biophys. Acta, 1974, vol. 333, pp. 52–58.CrossRefGoogle Scholar
  9. 9.
    Hipkins, M.F. and Barber, J., Estimation of the Activation Energy for Millisecond Delayed Fluorescence from Uncoupled Chloroplasts, FEBS Lett., 1974, vol. 42, pp. 289–292.PubMedCrossRefGoogle Scholar
  10. 10.
    Barber, J. and Neumann, J., An Energy Conservation Site between H2O and DBMIB: Evidence from msec Delayed Light and Chlorophyll Fluorescence Studies in Chloroplasts, FEBS Lett., 1974, vol. 40, pp. 186–189.CrossRefGoogle Scholar
  11. 11.
    Rutherford, A.W. and Inoue, Y., Oscillations of Delayed Luminescence from PSII: Recombination of S2QB-and S3QB, FEBS Lett., 1984, vol. 165, pp. 163–170.CrossRefGoogle Scholar
  12. 12.
    Govindjee and Papageorgiou, G., Chlorophyll Fluorescence and Photosynthesis; Fluorescence Transients, Photophysiology, Current Topics in Photobiology and Photochemistry, Giese, A.C., Ed., New York: Academic, 1971, pp. 1–47.Google Scholar
  13. 13.
    Papageorgiou, G., Chlorophyll Fluorescence: Intrinsic Probe of Photosynthesis, Bioenergetics of Photosynthesis, Govindjee, Ed., New York: Academic, 1975, pp. 319–371.Google Scholar
  14. 14.
    Jursinic, P., Delayed Fluorescence: Current Concepts and Status, Light Emission by Plants and Bacteria, Govindjee, Amesz, J., and Fork, D.C., Eds., Orlando, Florida: Academic, 1986, pp. 291–328.Google Scholar
  15. 15.
    Zaharieva, I., Taneva, S.G., and Goltsev, V., Effect of PSII Antennae Size on the Induction Kinetics of Prompt and Delayed Chlorophyll Fluorescence, Bulg. J. Plant Physiol., 1999, vol. 25, pp. 17–30.Google Scholar
  16. 16.
    Vučinić Ž., Temperature Dependence of the Steady-State Delayed Light Emission of Maize Leaf, Fiziol. Biochim. Kul’t. Rast., 1983, vol. 15, pp. 3–7.Google Scholar
  17. 17.
    Marković, D.Z., Jeremić, M.G., Radenović, Č.N., and Vučinić, Ž.B., A Study of Temperature Induced Structural Changes in Photosynthetic System Using Delayed Fluorescence, J. Serb. Chem. Soc., 1987, vol. 52, pp. 331–336.Google Scholar
  18. 18.
    Dzhibladze, T.G., Bukhov, N.G., and Karapetyan, N.V., Relations between Kinetic Curves of Variable Fluorescence and Decisecond Component of Delayed Fluorescence in Plant Leaves, Biofizika, 1988, vol. 33, pp. 121–125.Google Scholar
  19. 19.
    Bukhov, N.G., Rakhimberdieva, M.G., and Karapetyan, N.V., Nature of Slow Transient Phenomena of Variable and Delayed Fluorescence in Leaves, Fiziol. Rast. (Moscow), 1989, vol. 36, pp. 1045–1054 (Sov. Plant Physiol., Engl. Transl.).Google Scholar
  20. 20.
    Radenović, C., Jeremić, M., Fidler, D., Marković, D., and Vučinić, Ž., A Kinetic Study of Delayed Fluorescence Induction and Its Dependence on Preceding Darkness, Period. Biol., 1985, vol. 87, pp. 304–306.Google Scholar
  21. 21.
    Veselovskii, V.A. and Veselova, T.V., Luminescence of Plants, Theoretical and Applied Aspects, Moscow: Nauka, 1990.Google Scholar
  22. 22.
    Lichtenthaler, H.K. and Rinderle, U., The Role of Chlorophyll Fluorescence in the Detection of Stress Conditions in Plants, CRC Crit. Rev. Anal. Chem., 1988, vol. 19, pp. S29–S85.Google Scholar
  23. 23.
    Lichtenthaler, H.K., The Kautsky Effect: 60 Years of Chlorophyll Fluorescence Induction Kinetics, Photosynthetica, 1992, vol. 27, pp. 45–55.Google Scholar
  24. 24.
    Marković, D.Z., Kalauzi, A., and Radenović, Č.N., Delayed Fluorescence Induction Transients: Mathematical Modelling Based on the Chosen Kinetic Models, Gen. Physiol. Biophys., 2001, vol. 20, pp. 303–313.PubMedGoogle Scholar
  25. 25.
    Goltsev, V. and Yordanov, I., Mathematical Model of Prompt and Delayed Chlorophyll Fluorescence Induction Kinetics, Photosynthetica, 1997, vol. 33, pp. 571–586.Google Scholar
  26. 26.
    Goltsev, Y., Zaharieva, I., Lambrev, P., Yordanov, I., and Strasser, R., Simultaneous Analysis of Prompt and Delayed Chlorophyll a Fluorescence in Leaves during the Induction Period of Dark to Light Adaptation, J. Theor. Biol., 2003, vol. 225, pp. 171–183.PubMedCrossRefGoogle Scholar
  27. 27.
    Jursinic, P. and Govindjee, Effects of Hydroxylamine and Silicomolybdate on the Decay of Delayed Light Emission in the 6–100 msec Range after a Single 10 ns Flash in Pea Thylakoids, Photosynthetica, 1982, vol. 3, pp. 161–177.Google Scholar
  28. 28.
    Marković, D.Z., Radenović, Č.N., Rafailović, L., Žerajić, S.A., Jeremić, M., and Marković, M.B., Temperature Dependence of Delayed Fluorescence Induction Curve Transients, Gen. Physiol. Biophys., 1999, vol. 18, pp. 257–267.PubMedGoogle Scholar
  29. 29.
    McCauley, S.W. and Ruby, R.H., Delayed Fluorescence Induction in Chloroplasts. Irradiation Dependence, Biochim. Biophys. Acta, 1981, vol. 638, pp. 268–274.CrossRefGoogle Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2006

Authors and Affiliations

  • A. Kalauzi
    • 1
  • D. Z. Marković
    • 2
  • Č. N. Radenović
    • 3
  1. 1.Center for Multidisciplinary StudiesUniversity of BelgradeBelgradeSerbia and Montenegro
  2. 2.Faculty of TechnologyUniversity of NišLeskovacSerbia and Montenegro
  3. 3.Maize Research InstituteBelgrade-ZemunSerbia and Montenegro

Personalised recommendations