Changes in the Plasma Protein Composition in Cosmonauts after Space Flight and its Significance for Endothelial Functions
- 24 Downloads
Spaceflight (SF) conditions have a significant impact on the functioning of the human cardiovascular system. The endothelium plays an important role in the process of adaptation to SF factors. Therefore, the detection of biomarkers of endothelial dysfunction is necessary for understanding the molecular mechanisms involved in changes caused by SFs. For this purpose, the blood plasma proteins of 18 Russian cosmonauts were used for quantitative analysis by liquid chromatography with a UPLC 1290 Infinity chromatograph coupled to an Agilent 6490 triple quadrupole mass spectrometer. It was found that a decrease in the circulating plasma volume during the flight followed by the activation of fluid retention at the final stage of flight contributed to the changes in plasma protein concentrations on the first day after landing. We observed a significant increase in the concentration of the S100A9 protein that plays an important role in endothelium functioning and angiogenesis and can serve as a marker of inflammatory reactions. On the first day after landing, the complement system and acute-phase protein concentrations tended to increase, which can adversely affect the functioning of the endothelium.
Keywords:chromatography-mass spectrometry cosmonauts blood acute-phase proteins
This study was supported by the Russian Foundation for Basic Research, project no. 18-34-00524. The materials were prepared in the framework of RAS basic topics 65.3.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. All cosmonauts voluntarily filled in the informed consent form. The experiment was approved by the Biomedical Ethics Committee, Institute of Biomedical Problems, Russian Academy of Sciences, and the Human Research Multilateral Review Board.
- 1.Demiot, C., Dignat-George, F., Fortrat, J.O., et al., WISE 2005: chronic bed rest impairs microcirculatory endothelium in women, Am. J. Physiol.: Heart Circ. Physiol., 2007, vol. 293, no. 5, p. H3159.Google Scholar
- 11.Belova, L.A., Ogloblina, O.G., Satalkin, A.A., et al., Imbalance in the proteinase-inhibitor system in obstetric sepsis and septic shock, Klin. Lab. Diagn., 2003, no. 7, p. 13.Google Scholar
- 14.Kuzichkin, D.S., Morukov, B.V., Markin, A.A., et al., Cosmonauts homeostasis system indices after long-term and short-term space flights, 17th IAA Humans in Space Symp., June 7–11, 2009, Moscow, Russia, Moscow, 2009, p. 75.Google Scholar
- 16.Stein, T.P. and Leskiw, M.J., Oxidant damage during and after spaceflight, Am. J. Physiol.: Endocrinol. Metab., 2000, vol. 278, no. 3, p. E375.Google Scholar
- 18.Kozlov, A.A., Berkovich, A.L., Kachalova, N.D., et al., Posobie dlya vrachei-laborantov po metodam issledovaniya plazmennogo gemostaza. Faktory svertyvaniya krovi (Manual for Physicians-Laboratory Assistants for Analysis of Plasma Homeostasis. Blood Coagulation Factors), Moscow: Ross. Akad. Med. Nauk, 2006, p. 24.Google Scholar
- 19.Larina, O.N., Protein composition of astronaut’s blood plasma after prolonged orbital flights, Kosm. Biol. Aviakosm. Med., 1992, vol. 26, no. 3, p. 67.Google Scholar
- 23.Bartolomei, S., Sadres, E., Church, D.D., et al., Comparison of the recovery response from high-intensity and high-volume resistance exercise in trained men, Eur. J. Appl. Physiol., 2017, vol. 117, no. 7, p. 1287.Google Scholar
- 28.Li, C., Li, S., Jia, C., et al., Low concentration of S100A8/9 promotes angiogenesis-related activity of vascular endothelial cells: bridges among inflammation, angiogenesis, and tumorigenesis? Mediators Inflammation, 2012, vol. 2012, p. 248574.Google Scholar
- 29.Gliemann, L., Olesen, J., Bienso, R.S., et al., Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men, Am. J. Physiol.: Heart Circ. Physiol., 2014, vol. 307, no. 8, p. H1111.Google Scholar