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Human Physiology

, Volume 45, Issue 1, pp 75–82 | Cite as

Changes in the Plasma Protein Composition in Cosmonauts after Space Flight and its Significance for Endothelial Functions

  • D. N. KashirinaEmail author
  • L. Kh. Pastushkova
  • A. J. Percy
  • Ch. H. Borchers
  • A. G. Brzhozovsky
  • I. M. Larina
Article
  • 24 Downloads

Abstract

Spaceflight (SF) conditions have a significant impact on the functioning of the human cardiovascular system. The endothelium plays an important role in the process of adaptation to SF factors. Therefore, the detection of biomarkers of endothelial dysfunction is necessary for understanding the molecular mechanisms involved in changes caused by SFs. For this purpose, the blood plasma proteins of 18 Russian cosmonauts were used for quantitative analysis by liquid chromatography with a UPLC 1290 Infinity chromatograph coupled to an Agilent 6490 triple quadrupole mass spectrometer. It was found that a decrease in the circulating plasma volume during the flight followed by the activation of fluid retention at the final stage of flight contributed to the changes in plasma protein concentrations on the first day after landing. We observed a significant increase in the concentration of the S100A9 protein that plays an important role in endothelium functioning and angiogenesis and can serve as a marker of inflammatory reactions. On the first day after landing, the complement system and acute-phase protein concentrations tended to increase, which can adversely affect the functioning of the endothelium.

Keywords:

chromatography-mass spectrometry cosmonauts blood acute-phase proteins 

Notes

ACKNOWLEDGMENTS

This study was supported by the Russian Foundation for Basic Research, project no. 18-34-00524. The materials were prepared in the framework of RAS basic topics 65.3.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All cosmonauts voluntarily filled in the informed consent form. The experiment was approved by the Biomedical Ethics Committee, Institute of Biomedical Problems, Russian Academy of Sciences, and the Human Research Multilateral Review Board.

REFERENCES

  1. 1.
    Demiot, C., Dignat-George, F., Fortrat, J.O., et al., WISE 2005: chronic bed rest impairs microcirculatory endothelium in women, Am. J. Physiol.: Heart Circ. Physiol., 2007, vol. 293, no. 5, p. H3159.Google Scholar
  2. 2.
    Watenpaugh, D.E., Fluid volume control during short-term spaceflight and implications for human performance, J. Exp. Biol., 2001, vol. 204, no. 18, p. 3209.PubMedGoogle Scholar
  3. 3.
    Kuzyk, M.A., Parker, C.E., Domanski, D., and Borchers, C.H., Development of MRM-based assays for the absolute quantitation of plasma proteins, Methods Mol. Biol., 2013, vol. 1023, p. 53.CrossRefPubMedGoogle Scholar
  4. 4.
    Larina, I.M., Percy, A.J., Yang, J., et al., Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts, Sci. Rep., 2017, vol. 7, p. 1.CrossRefGoogle Scholar
  5. 5.
    Schiopu, A. and Cotoi, O.S., S100A8 and S100A9: DAMPs at the crossroads between innate immunity, traditional risk factors, and cardiovascular disease, Mediators Inflammation, 2013, vol. 2013, p. 828 354.CrossRefGoogle Scholar
  6. 6.
    Cagnin, S., Biscuola, M., Patuzzo, C., et al., Reconstruction and functional analysis of altered molecular pathways in human atherosclerotic arteries, BMC Genomics, 2009, vol. 10, no. 1, p. 13.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Croce, K., Gao, H., Wang, Y., et al., Myeloid-related protein-8/14 is critical for the biological response to vascular injury, Circulation, 2009, vol. 120, no. 5, p. 427.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Gazenko, O.G., Grigor’ev, A.I., and Natochin, Yu.V., Water-salt homeostasis and weightlessness, Kosm. Biol. Aviakosm. Med., 1980, vol. 14, no. 5, p. 3.PubMedGoogle Scholar
  9. 9.
    Grigor’ev, A.I., Larina, I.M., and Noskov, V.B., Influence of space flights on the state and regulation of water–electrolyte homeostasis, Ross. Fiziol. Zh. im. I.M. Sechenova, 2006, vol. 92, no. 1, p. 5.PubMedGoogle Scholar
  10. 10.
    Leach, C.S., Alfrey, C.P., Suki, W.N., et al., Regulation of body fluid compartments during short-term spaceflight, J. Appl. Physiol., 1996, vol. 81, p. 105.CrossRefPubMedGoogle Scholar
  11. 11.
    Belova, L.A., Ogloblina, O.G., Satalkin, A.A., et al., Imbalance in the proteinase-inhibitor system in obstetric sepsis and septic shock, Klin. Lab. Diagn., 2003, no. 7, p. 13.Google Scholar
  12. 12.
    Popov, I.G. and Latskevich, A.A., Amino acids in the astronauts’ blood before and after the 211-day flight, Kosm. Biol. Aviakosm. Med., 1984, vol. 18, no. 2, p. 26.PubMedGoogle Scholar
  13. 13.
    Kaur, I., Simons, E.R., Kapadia, A.S., et al., Effect of spaceflight on ability of monocytes to respond to endotoxins of gram-negative bacteria, Clin. Vaccine Immunol., 2008, vol. 15, no. 10, p. 1523.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Kuzichkin, D.S., Morukov, B.V., Markin, A.A., et al., Cosmonauts homeostasis system indices after long-term and short-term space flights, 17th IAA Humans in Space Symp., June 7–11, 2009, Moscow, Russia, Moscow, 2009, p. 75.Google Scholar
  15. 15.
    Fomin, A.N., Characteristics of blood fibrinogen in 7-day water immersion and short-term space flight, Kosm. Biol. Aviakosm. Med., 1981, vol. 15, no. 5, p. 83.PubMedGoogle Scholar
  16. 16.
    Stein, T.P. and Leskiw, M.J., Oxidant damage during and after spaceflight, Am. J. Physiol.: Endocrinol. Metab., 2000, vol. 278, no. 3, p. E375.Google Scholar
  17. 17.
    Steptoe, A., Kivimaki, M., Lowe, G., et al., Blood pressure and fibrinogen responses to mental stress as predictors of incident hypertension over an 8-year period, Ann. Behav. Med., 2016, vol. 50, no. 6, p. 898.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Kozlov, A.A., Berkovich, A.L., Kachalova, N.D., et al., Posobie dlya vrachei-laborantov po metodam issledovaniya plazmennogo gemostaza. Faktory svertyvaniya krovi (Manual for Physicians-Laboratory Assistants for Analysis of Plasma Homeostasis. Blood Coagulation Factors), Moscow: Ross. Akad. Med. Nauk, 2006, p. 24.Google Scholar
  19. 19.
    Larina, O.N., Protein composition of astronaut’s blood plasma after prolonged orbital flights, Kosm. Biol. Aviakosm. Med., 1992, vol. 26, no. 3, p. 67.Google Scholar
  20. 20.
    Muid, S., Froemming, G.R., Ali, A.M., and Nawawi, H., Interleukin-6 and intercellular cell adhesion molecule-1 expression remains elevated in revived live endothelial cells following spaceflight, Malays J. Pathol., 2013, vol. 35, no. 2, p. 165.PubMedGoogle Scholar
  21. 21.
    Stein, T.P. and Schluter, M.D., Excretion of IL-6 by astronauts during spaceflight, Am. J. Physiol., 1994, vol. 266, p. E448.CrossRefPubMedGoogle Scholar
  22. 22.
    Crucian, B.E., Zwart, S.R., Mehta, S., et al., Plasma cytokine concentrations indicate that in vivo hormonal regulation of immunity is altered during long-duration spaceflight, J. Interferon Cytokine Res., 2014, vol. 34, no. 10, p. 778.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Bartolomei, S., Sadres, E., Church, D.D., et al., Comparison of the recovery response from high-intensity and high-volume resistance exercise in trained men, Eur. J. Appl. Physiol., 2017, vol. 117, no. 7, p. 1287.Google Scholar
  24. 24.
    Serrano, A.L., Baeza-Raja, B., Perdiguero, E., et al., Interleukin-6 is an essential regulator of satellite cell-mediated skeletal muscle hypertrophy, Cell Metab., 2008, vol. 7, no. 1, p. 33.CrossRefGoogle Scholar
  25. 25.
    Wang, S.W. and Sun, Y.M., The IL-6/JAK/STAT3 pathway: potential therapeutic strategies in treating colorectal cancer (review), Int. J. Oncol., 2014, vol. 44, no. 4, p. 1032.CrossRefPubMedGoogle Scholar
  26. 26.
    Nishikawa, Y., Kajiura, Y., Lew, J.H., et al., Calprotectin induces IL-6 and MCP-1 production via Toll-like receptor 4 signaling in human gingival fibroblasts, J. Cell. Physiol., 2017, vol. 232, no. 7, p. 1862.CrossRefPubMedGoogle Scholar
  27. 27.
    Ehlermann, P., Eggers, K., Bierhaus, A., et al., Increased proinflammatory endothelial response to S100A8/A9 after preactivation through advanced glycation end products, Cardiovasc. Diabetol., 2006, vol. 30, no. 5, p. 6.CrossRefGoogle Scholar
  28. 28.
    Li, C., Li, S., Jia, C., et al., Low concentration of S100A8/9 promotes angiogenesis-related activity of vascular endothelial cells: bridges among inflammation, angiogenesis, and tumorigenesis? Mediators Inflammation, 2012, vol. 2012, p. 248574.Google Scholar
  29. 29.
    Gliemann, L., Olesen, J., Bienso, R.S., et al., Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men, Am. J. Physiol.: Heart Circ. Physiol., 2014, vol. 307, no. 8, p. H1111.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • D. N. Kashirina
    • 1
    Email author
  • L. Kh. Pastushkova
    • 1
  • A. J. Percy
    • 2
  • Ch. H. Borchers
    • 2
  • A. G. Brzhozovsky
    • 1
  • I. M. Larina
    • 1
  1. 1.Institute of Biomedical Problems, Russian Academy of SciencesMoscowRussia
  2. 2.University of Victoria, Victoria V8P 5C2Canada

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