Russian Journal of Physical Chemistry A

, Volume 81, Issue 8, pp 1335–1340 | Cite as

Aggregation and photobleaching of merocyanine 540 as probed by resonance light scattering

  • E. A. Kozhinova
  • A. M. Tikhomirova
  • L. A. Kozyr’
  • A. A. Kyagova
  • A. Ya. Potapenko
Photochemistry and Magnetochemistry

Abstract

Merocyanine 540 (MC540) aggregation induced by adding KCl has been studied by resonance light scattering (RLS) and absorption spectrophotometry. In water, MC540 exists as H-dimers and monomers with absorption maxima at ∼500 and 535 nm, respectively (these species lack RLS). Upon the introduction of the salt in concentrations below 0.15 M, the spectrum undergoes a hypochromic effect, without modification of its general shape. In addition to the hypochromic effect, a new broad RLS band emerges at ∼420–460 nm, which arises from large H-aggregates of the dye. The formation of these aggregates does not manifest itself in absorption spectra. At KCl concentrations above the critical one (0.15 M), a new absorption band at ∼517 nm emerges; simultaneously, a strong RLS band of a similar shape appears, which proves the formation of large supramolecular aggregates of MC540. Comparison of the spectrophotometry and RLS data shows that large aggregates of MC540 are more photolabile than dimers and monomers.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    L. Šikurová, B. Čunderlíková, J. Turisová, and I. Waczuliková, Anal. Chim. Acta 303(1), 79 (1995).CrossRefGoogle Scholar
  2. 2.
    D. Bernik, E. Tymczyszyn, M. E. Daraio, and R. M. Negri, Photochem. Photobiol. 70(1), 40 (1999).CrossRefGoogle Scholar
  3. 3.
    A. Mateasik, L. Šikurová, and D. Chorvát, Jr., Bioelectrochem. 55(1–2), 173 (2002).CrossRefGoogle Scholar
  4. 4.
    K. S. Gulliya, S. Pervaiz, R. M. Dowben, and J. L. Matthews, Photochem. Photobiol. 52(4), 831 (1990).Google Scholar
  5. 5.
    A. Adenier and J. J. Aaron, Spectrochim. Acta, Part A 58(3), 543 (2002).CrossRefGoogle Scholar
  6. 6.
    L. Šikurová, I. Haban, and D. Chorvát, Stud. Biophys. 125, 197 (1988).Google Scholar
  7. 7.
    E. A. Kozhinova, L. A. Kozyr’, A. M. Tikhomirov, et al., Vestn. Ros. Gos. Med. Univ., No. 7, 47 (2005).Google Scholar
  8. 8.
    R. J. Singh, J. B. Feix, T. J. Pintar, et al., Photochem. Photobiol. 53(4), 493 (1991).Google Scholar
  9. 9.
    M. Hoebeke, A. Seret, J. Piette, and A. van de Vorst, J. Photochem. Photobiol., B 1(4), 437 (1988).CrossRefGoogle Scholar
  10. 10.
    P. Bilski, T. McDevitt, and C. F. Chignell, Photochem. Photobiol. 69(6), 671 (1999).CrossRefGoogle Scholar
  11. 11.
    R. J. Singh, J. B. Feix, and B. Kalyanaraman, Photochem. Photobiol. 55(4), 483 (1992).Google Scholar
  12. 12.
    S. Pervaiz, J. L. Hirpara, and M. V. Clement, Cancer Lett. 128(1), 11 (1998).CrossRefGoogle Scholar
  13. 13.
    A. A. Kyagova, L. A. Kozir, G. V. Mansurova, et al., Russ. J. Immunol. 7(4), 327 (2002).Google Scholar
  14. 14.
    R. F. Pasternack and P. J. Collings, Science (Washington, D.C.) 269(5226), 935 (1995).CrossRefGoogle Scholar
  15. 15.
    J. Parkash, J. H. Robblee, J. Agnew, et al., Biophys. J. 74(4), 2089 (1998).CrossRefGoogle Scholar
  16. 16.
    L. P. Aggarwal and I. E. Borissevitch, Spectrochim. Acta, Part A 63(1), 227 (2006).CrossRefGoogle Scholar
  17. 17.
    I. E. Borissevitch, T. T. Tominaga, H. Imasato, and M. Tabak, Anal. Chim. Acta 343(3), 281 (1997).CrossRefGoogle Scholar
  18. 18.
    C. Z. Huang, Y. Liu, Y. H. Wang, and H. P. Guo, Anal. Biochem. 321(2), 236 (2003).CrossRefGoogle Scholar
  19. 19.
    R. F. Pasternack, Chirality 15(4), 329 (2003).CrossRefGoogle Scholar
  20. 20.
    B. Čunderlíková and L. Šikurová, Chem. Phys. 263(2–3), 415 (2001).CrossRefGoogle Scholar
  21. 21.
    L. Šikurová and B. Čunderlíková, Spectrochim. Acta, Part A 53(2), 293 (1997).CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • E. A. Kozhinova
    • 1
  • A. M. Tikhomirova
    • 1
  • L. A. Kozyr’
    • 1
  • A. A. Kyagova
    • 1
  • A. Ya. Potapenko
    • 1
  1. 1.Russian State Medical UniversityMoscowRussia

Personalised recommendations