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Journal of Ichthyology

, Volume 58, Issue 3, pp 312–317 | Cite as

First Record of African Angel Shark, Squatina africana (Chondricthyes: Squatinidae) in Indian Waters, Confirmed by DNA Barcoding

  • M. N. Ambily
  • P. U. ZachariaEmail author
  • T. M. Najmudeen
  • L. Ambily
  • K. T. S. Sunil
  • M. Radhakrishnan
  • T. G. Kishor
Article
  • 33 Downloads

Abstract

A single specimen of African angel shark, Squatina africana (Regan, 1908) was caught off Lakshadweep (11°5′47″ N; 72°2′21″ E), India in September 2016. The present study is a new report of the above species from Indian waters. In addition to classical methodologies, DNA barcoding was also adopted for species identification. The 650 bp-long region of mitochondrial Cytochrome Oxidase subunit I was sequenced to obtain the DNA barcode for the species under study. The sequence divergence value within species and between species was calculated using MEGA V.7.0, where Kimura 2 parameter (k2p) model was chosen as a distance model. The average k2p distance separating individuals within species was 1.76% and inter specific divergence was 8–10%. A neighbour joining network was constructed to provide a graphical representation of divergence between the species. Using the maximum identity with Gen Bank database, K2P divergence distance, NJ-network and traditional morphological approach, we could identify the given specimen as a mature male African angel shark.

Keywords

first record Squatinidae cytochrome c oxidase Squatina africana India 

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • M. N. Ambily
    • 1
  • P. U. Zacharia
    • 1
    Email author
  • T. M. Najmudeen
    • 1
  • L. Ambily
    • 1
  • K. T. S. Sunil
    • 1
  • M. Radhakrishnan
    • 1
  • T. G. Kishor
    • 1
  1. 1.ICAR-Central Marine Fisheries Research Institute (CMFRI)KochiIndia

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