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Molecular Biology

, Volume 53, Issue 4, pp 535–546 | Cite as

Evolution and Comparative Genomics of the pSM22 Plasmid of the IncF/MOBF12 Group

  • T. R. IasakovEmail author
  • L. G. Anisimova
  • N. V. Zharikova
  • E. I. Zhurenko
  • V. V. Korobov
  • T. V. Markusheva
GENOMICS. TRANSCRIPTOMICS
  • 1 Downloads

Abstract

A new plasmid, pSM22, was isolated from Serratia marcescens and sequenced. Its 43 190-bp sequence with an average GC-content of 58% contains 31 open reading frames (ORFs) which form replication, conjugation, stability, and adaptive modules. The replication module includes a site of initiation of leading-strand synthesis in plasmid replication, a replication termination site (terC), the repA (=repA1) and repA4 genes, and the copA sequence, which codes for an antisense RNA (asRNA). These structures are functionally integrated in an FII replicon (incompatibility group IncFII). Based on the significant differences between the FII replicon and the canonical sequences of the R plasmids R1 and NR1 (=R100=R222), pSM22 was assigned to a new subtype. The conjugation module includes 13 genes with a high identity to the genes responsible for conjugation of the F plasmid. A comparative genomic analysis showed that the conjugation modules of pSM22 and F are structurally similar. By the conjugation system and the presence of three conserved motifs in relaxase (TraI), pSM22 belongs to the F12 clade of the MOBF type. The stability module includes the resD and parA genes, which are responsible for the resolution of multimeric plasmid forms and their subsequent segregation between daughter cells. The adaptive module contains the microcin H47 (MccH47) secretion/processing and UV resistance genes. The mosaic structure of pSM22 and reductive evolution of its modules suggest high genomic plasticity for the genus Serratia. An analysis of the architecture of the pSM22 modules clarifies the evolutionary relationships among IncF/MOBF12 group plasmids in bacteria of the family Enterobacteriaceae and opens a novel avenue for further comparative genomic studies of Serratia plasmids.

Keywords:

plasmid pSM22 IncF/MOBF12 ori repA evolution comparative genomics Serratia marcescens replicon relaxase 

Notes

ACKNOWLEDGMENTS

We are grateful to B.B. Kuznetsov, B.K. Bumazhkin, T.V. Kolganova, and E.O. Patutina (Institute of Bioengineering, Russian Academy of Sciences, Moscow) for help in experiments.

The study was carried out using the infrastructure and equipment of the Core Facilities “Bioengineering” (Moscow) and “Agidel” (Ufa).

FUNDING

This work was supported by a Government Contract, state registration no. AAAA-A18-118022190098-9.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.

Statement of compliance with standards of research involving humans as subjects. This article does not contain any studies involving humans performed by any of the authors.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • T. R. Iasakov
    • 1
    Email author
  • L. G. Anisimova
    • 1
  • N. V. Zharikova
    • 1
  • E. I. Zhurenko
    • 1
  • V. V. Korobov
    • 1
  • T. V. Markusheva
    • 1
  1. 1.Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of SciencesUfaRussia

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