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Molecular Biology

, Volume 53, Issue 4, pp 501–512 | Cite as

Elongation Factor P: New Mechanisms of Function and an Evolutionary Diversity of Translation Regulation

  • A. A. Golubev
  • Sh. Z. Validov
  • K. S. Usachev
  • M. M. YusupovEmail author
REVIEWS
  • 6 Downloads

Abstract

The protein synthesis in cells occurs in ribosomes, with the involvement of protein translational factors. One of these translational factors is the elongation factor P (EF-P). EF-P is a three-domain protein that binds between the P and E sites of the ribosome, near the P-tRNA, the peptidyl transferase center, and E-site codon of the mRNA. The majority of studies showed that the EF-P helps the ribosome to synthesize stalling amino acid motifs, such as polyprolines. In the first part of this review, we inspect the general evolutionary variety of the EF-P in different organisms, the problems of the regulation provided by the EF-P, and its role in the sustainability of the protein balance in the cell in different physiological states. Although the functions of the EF-P have been well studied, there are still some problems that remain to be solved. The data from recent studies contradict the previous theories. Consequently, in the second part, we discuss the recent data that suggest the involvement of the EF-P in each translocation event, not only in those related to polyproline synthesis. This activity contradicts some aspects of the known pathway of the removal of the E-tRNA during the translocation event. In addition, in the third part of this review, we tried to partly shift the interest from the antistalling activity of domain I of the EF-P to the action of domain III, the functions of which has not been closely studied. We expand on the idea about the involvement of domain III of the EF-P in preventing the frameshift and debate the EF-P’s evolutionary history.

Keywords:

ribosome EF-P translation 

Notes

FUNDING

This study was supported by the Russian Science Foundation (project no. 17-74-20009).

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interest. The authors declare that they have no conflict of interest.

Statement of the welfare of animals. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • A. A. Golubev
    • 1
  • Sh. Z. Validov
    • 1
  • K. S. Usachev
    • 1
  • M. M. Yusupov
    • 1
    • 2
    Email author
  1. 1.Institute of Fundamental Medicine and Biology, Kazan (Volga) Federal UniversityKazanRussia
  2. 2.Institut de Génétique et de Biologie Moléculaire et CellulaireStrasbourgFrance

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