Molecular Biology

, Volume 53, Issue 3, pp 438–441 | Cite as

The Involvement of Cardiomyocyte-Specific Transcription Factors Meis in Adipocyte Differentiation

  • A. Greco
  • D. V. Vaipan
  • V. A. Tkachuk
  • D. N. PenkovEmail author


Homeodomain transcription factors play a significant role in adipocyte differentiation. The role of Pbx1 and Prep1, proteins of the TALE family (the three amino acid loop extension), was previously established in adipocyte differentiation of mesenchymal stromal cells and 3T3-L1 cell line. In this study, with the use of RNA interference technology we show that another transcription factor from the same family, Meis1, which is a core protein of mature cardiomyocytes, represses adipogenesis to a greater degree than its paralog Meis2. A number of Meis target genes, markers of adipocytes, are identified. This may indicate the transcriptional mechanism of the effect of Meis1 on the adipocyte differentiation of mouse preadipocytes.


adipocyte differentiation transcription factor chromatin Meis1 Meis2 



  1. 1.
    Siersbæk R., Nielsen R., John S., Sung M.H., Baek S., Loft A., Hager G.L., Mandrup S. 2011. Extensive chromatin remodelling and establishment of transcription factor “hotspots” during early adipogenesis. EMBO J. 30, 1459–1472.CrossRefGoogle Scholar
  2. 2.
    Siersbaek R., Nielsen R., Mandrup S. 2012. Transcriptional networks and chromatin remodeling controlling adipogenesis. Trends Endocrinol. Metab. 23, 56–64.CrossRefGoogle Scholar
  3. 3.
    Fu M., Sun T., Bookout A.L., Downes M., Yu R.T., Evans R.M., Mangelsdorf D.J. 2005. A nuclear receptor atlas: 3T3-L1 adipogenesis. Mol. Endocrinol. 19, 2437–2450.CrossRefGoogle Scholar
  4. 4.
    Siersbæk R., Baek S., Rabiee A., Nielsen R., Traynor S., Clark N., Sandelin A., Jensen O.N., Sung M.H., Hager G.L., Mandrup S. 2014. Molecular architecture of transcription factor hotspots in early adipogenesis. Cell Rep. 7, 1434–1442.CrossRefGoogle Scholar
  5. 5.
    Monteiro M.C., Sanyal M., Cleary M.L., Sengenès C., Bouloumié A., Dani C., Billon N. 2011. PBX1: A novel stage-specific regulator of adipocyte development. Stem Cells. 29, 1837–1848.CrossRefGoogle Scholar
  6. 6.
    Penkov D., Mateos San Martín D., Fernandez-Díaz L.C., Rosselló C.A., Torroja C., Sánchez-Cabo F., Warnatz H.J., Sultan M., Yaspo M.L., Gabrieli A., Tkachuk V., Brendolan A., Blasi F., Torres M. 2013. Analysis of the DNA-binding profile and function of TALE homeoproteins reveals their specialization and specific interactions with Hox genes/proteins. Cell Rep. 3, 1321–1333.CrossRefGoogle Scholar
  7. 7.
    Maroni G., Tkachuk V.A., Egorov A., Morelli M.J., Luongo R., Levantini E., Blasi F., Magli M.C., Penkov D. 2017. Prep1 prevents premature adipogenesis of mesenchymal progenitors. Sci. Rep. 7, 15573.CrossRefGoogle Scholar
  8. 8.
    Liu Q., Jiang C., Xu J., Zhao M.T., Van Bortle K., Cheng X., Wang G., Chang H.Y., Wu J.C., Snyder M.P. 2017. Genome-wide temporal profiling of transcriptome and open chromatin of early cardiomyocyte differentiation derived from hiPSCs and hESCs. Circ. Res. 121, 376–391.CrossRefGoogle Scholar
  9. 9.
    Quaranta R., Fell J., Rühle F., Rao J., Piccini I., Araúzo-Bravo M.J., Verkerk A.O., Stoll M., Greber B. 2018. Revised roles of ISL1 in a hES cell-based model of human heart chamber specification. eLife. 7, e31706.CrossRefGoogle Scholar
  10. 10.
    Moffat J., Grueneberg D.A., Yang X., Kim S.Y., Kloepfer A.M., Hinkle G., Piqani B., Eisenhaure T.M., Luo B., Grenier J.K., Carpenter A.E., Foo S.Y., Stewart S.A., Stockwell B.R., Hacohen N., et al. 2006. A lentiviral RNAi libraryfor human and mouse genes applied to an arrayed viral high-content screen. Cell. 124, 1283–1298.CrossRefGoogle Scholar
  11. 11.
    Longobardi E., Blasi F. 2003. Overexpression of PREP-1 in F9 teratocarcinoma cells leads to a functionally relevant increase of PBX-2 by preventing its degradation. J. Biol. Chem. 278, 39235–39241.CrossRefGoogle Scholar
  12. 12.
    Kraus N.A., Ehebauer F., Zapp B., Rudolphi B., Kraus B.J., Kraus D. 2016. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte. 5, 351–358.CrossRefGoogle Scholar
  13. 13.
    McLean C.Y., Bristor D., Hiller M., Clarke S.L., Schaar B.T., Lowe C.B., Wenger A.M., Bejerano G. 2010. GREAT improves functional interpretation of cis-regulatory regions. Nat. Biotechnol. 28, 495–501.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • A. Greco
    • 1
  • D. V. Vaipan
    • 2
  • V. A. Tkachuk
    • 2
    • 3
  • D. N. Penkov
    • 1
    • 3
    Email author
  1. 1.The FIRC Institute of Molecular OncologyMilanItaly
  2. 2.Moscow State UniversityMoscowRussia
  3. 3.Russian National Research Center for CardiologyMoscowRussia

Personalised recommendations