Molecular Biology

, Volume 53, Issue 2, pp 308–311 | Cite as

Protein Biosynthesis Proofreading Is Closely Associated with the Existence of Factor-Free Ribosomal Synthesis

  • A. V. FinkelsteinEmail author
  • L. P. Gavrilova


Despite protein biosynthesis being studied for decades, some major questions concerning this process are still to be addressed. We elucidate a close connection between proofreading of the emerging amino acid sequence during its normal, elongation factor-dependent ribosomal biosynthesis and the existence of the factor-free synthesis of a polypeptide chain on a ribosome. In this factor-free process, the biological role of proofreading is played by a process opposite to the factor-free attachment of Aa-tRNA to the ribosome, namely, the removal via the same pathway of that Aa-tRNA, which is not complementary to the mRNA codon exhibited by the ribosome.


biosynthesis of polypeptides ribosome elongation factor Tu GTP factor-free process parallel reactions free energy codon recognition proofreading 



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© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  1. 1.Institute of Protein Research, Pushchino, Russian Academy of SciencesPushchinoRussia
  2. 2.Faculty of Biology, Moscow State UniversityMoscowRussia

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