Transgenic Expression of chit42 gene from Metarhiziumanisopliae in Trichoderma harzianum Enhances Antagonistic Activity against Botrytis cinerea
Сhitinases expressed by some beneficial fungi are crucial for the biocontrol of phytopathogens. The activity of chitinolytic strains of Trichoderma sp. may be enhanced by increasing the expression of chitinases. We describe the Trichoderma strain Mchit42 which expresses a transgenic chitinase chit42 from Metarhizium anisopliae. Inhibitory effects against plant pathogens were tested. Comparison of WT (T30) and OE (Mchit42) indicated that overexpression of M. anisopliaechit42 did not alter Trichoderma growth, while enhancing the expression of endogenous chitinase, β-1,3-glucanases, and polygalacturonase and increasing the antagonistic activity of Trichoderma against Botrytis cinerea. This work confirmed that the expression of the entomopathogenic fungi-sourced chit42 genes in Trichodermaharzianum enhances the efficiency of Trichoderma biocontrol against targeted pathogens.
Keywords:chit42 gene Metarhizium anisopliae Trichoderma harzianum antagonistic activity Botrytis cinerea
This work was supported by National Key Research and Development Program of China (2017YFD0200901 and 2017YFD0200400), National Natural Science Foundation of China (31672072) and Agricultural Extension Project of Shanghai Municipal Agricultural Commission (2017) no. 1-6. We thank the laboratory of Plant Pathology of Shanghai Jiaotong University for providing us Metarhizium anisopliae CY1 and Trichoderma harzianum T30.
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