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Molecular Biology

, Volume 52, Issue 5, pp 701–706 | Cite as

Expression of Stroma Components in the Lymph Nodes Affected by Prostate Cancer Metastases

  • M. Yu. Shkurnikov
  • D. V. Maltseva
  • E. N. Knyazev
  • B. Ya. Alekseev
MOLECULAR CELL BIOLOGY
  • 34 Downloads

Abstract

The architecture of stroma is crucial for normal lymph node functioning, as well as for the systemic and local immune response. Data from previous studies in metastatic lymph nodes suggest that changes in the composition of extracellular matrix proteins may occur, not only around the lesion site, but throughout the lymph node stroma. In the present study, the extracellular matrix status was compared between the affected and metastasis-free lymph nodes in prostate cancer. It was found that the presence of tumor cells was associated with significant changes in the expression of genes encoding extracellular matrix components, including α4, β1 and γ1 laminin chains, osteonectin, and collagen, as well as with decrease in the expression of lymphatic endothelial cell biomarkers LYVE1 and NRP2. This result suggests that the normal stromal architecture is significantly disrupted in metastatic lymph nodes and may indicate the development of immune tolerance to the tumor cells.

Keywords:

lymphogenic metastasis lymph node prostate cancer α4 laminin chain LAMA4 LAMB1 LAMC1 laminin 411 (laminin-8) SPARC chemokine 

Notes

ACKNOWLEDGMENTS

This work was supported by the Russian Science Foundation (Agreements nos. 16-15-00290 and 17-14-01338). Analysis of laminin gene expression by real-time PCR was financed via Agreement no. 17-14-01338; the other experiments were supported via Agreement no. 16-15-00290.

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • M. Yu. Shkurnikov
    • 1
  • D. V. Maltseva
    • 2
  • E. N. Knyazev
    • 2
  • B. Ya. Alekseev
    • 1
  1. 1.National Medical Radiological Research Center, Ministry of Health of the Russian FederationObninskRussia
  2. 2.Bioclinicum Research CenterMoscowRussia

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