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Molecular Biology

, Volume 52, Issue 4, pp 583–589 | Cite as

Ribosome Inactivation and the Integrity of the Intestinal Epithelial Barrier

  • S. V. Nikulin
  • N. A. Mnafki (Krainova)
  • S. A. Shilin
  • I. N. Gazizov
  • D. V. Maltseva
Molecular Cell Biology

Abstract

The mistletoe lectin viscumin (MLI) is a ribosome-inactivating protein from Viscum album widely used in cancer therapy. Its antitumor properties are due to its immunomodulating action, previously demonstrated in experiments involving intravenous, subcutaneous, and oral administration of viscumin. To investigate whether viscumin has a cytotoxic effect on the intestinal epithelium, its safety was assessed using (i) impedance spectroscopy to measure the integrity of the colorectal adenocarcinoma Caco-2 cell monolayer after exposure to viscumin and (ii) a novel technique of determining the portion of viscumin-inactivated ribosomes. It was shown that inactivation of at least 20% of the ribosomes within 6 h did not lead to disruption of the Caco-2 cell monolayer or alter the physicochemical parameters of enterocyte membranes.

Keywords

MLI viscumin ricin ribosome-inactivating protein impedance spectroscopy TEER Caco-2 

Abbreviations

AP site

apurinic site

qPCR

quantitative realtime PCR

RIP-II

ribosome-inactivating protein type II

ER stress

endoplasmic reticulum stress

TEER

transepithelial electrical resistance

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • S. V. Nikulin
    • 1
    • 2
  • N. A. Mnafki (Krainova)
    • 1
  • S. A. Shilin
    • 1
  • I. N. Gazizov
    • 1
  • D. V. Maltseva
    • 1
  1. 1.Scientific Research Center BioclinicumMoscowRussia
  2. 2.Moscow Institute of Physics and Technology (State University)MoscowRussia

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