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Molecular Biology

, Volume 52, Issue 4, pp 548–555 | Cite as

Modification of Anti-Glycan IgG and IgM Profiles in Allergic Inflammation

  • V. I. Butvilovskaya
  • O. V. Smoldovskaya
  • G. U. Feyzkhanova
  • M. A. Filippova
  • L.V. Pavlushkina
  • S. A. Voloshin
  • A. Yu. Rubina
Molecular Cell Biology

Abstract

Glycans and anti-glycan antibodies (AGAs) are essential for infiltration of inflammatory cells in various allergies. The glycocalyx structure of the cells is modified during disease progression, and this modification is possible to evaluate by assessment of AGAs. A printed glycan array with 55 immobilized glycans and immobilized antibodies to IgG, IgA, and IgM was used to study the changes in AGA profiles in bronchial asthma (BA). Levels of antibodies to certain glycans in BA patients statistically differed from levels in healthy donors (p < 0.0007 by the Mann–Whitney test); the glycan set included 6Su-6`-SiaLec, Sia LeX, Sia6Htype2; Tαα, Manβ1-4GlcNAc, and Manα1-4Manβ. The obtained results help to better understand the mechanisms of the cell-mediated immune response in bronchial asthma and other types of allergic reactions.

Keywords

allergy bronchial asthma anti-glycan antibodies glycan array immune response siglecs galectins 

Abbreviations

GBP

glycan-binding protein

AGA

anti-glycan antibody

AGA-IgM and AGA-IgG

class M and class G AGAs, respectively

BA

bronchial asthma

PBS

phosphate-buffered saline

PBST

PBS supplemented with Tween 20 (washing buffer)

PVA

polyvinyl alcohol

PVP

polyvinylpyrrolidone

PBSP

PBS supplemented with PVA (blocking buffer)

DB

dilution buffer

BS

background signal

Cy5 and Cy3

cyanine dies 5 and 3, respectively

Su

sulfate

Sia

sialic acid

LN

lactosamine

DAMP

damage-associated molecular pattern

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • V. I. Butvilovskaya
    • 1
  • O. V. Smoldovskaya
    • 1
  • G. U. Feyzkhanova
    • 1
  • M. A. Filippova
    • 1
  • L.V. Pavlushkina
    • 2
  • S. A. Voloshin
    • 1
  • A. Yu. Rubina
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Filatov Moscow Pediatric Clinic no. 13Healthcare Department of MoscowMoscowRussia

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