Molecular Biology

, Volume 52, Issue 2, pp 190–199 | Cite as

Overexpression of microRNAs miR-9, -98, and -199 Correlates with the Downregulation of HK2 Expression in Colorectal Cancer

  • A. V. Snezhkina
  • G. S. Krasnov
  • S. O. Zhikrivetskaya
  • I. Y. Karpova
  • M. S. Fedorova
  • K. M. Nyushko
  • M. M. Belyakov
  • N. V. Gnuchev
  • D. V. Sidorov
  • B. Y. Alekseev
  • N. V. Melnikova
  • A. V. Kudryavtseva
Genomics. Transcriptomics
  • 17 Downloads

Abstract

Glycolysis activation is one of the main features of energy metabolism in cancer cells that is associated with the increase in glycolytic enzyme synthesis, primarily, hexokinases (HKs), in many types of tumors. Conversely, in colorectal cancer (CRC) the decrease in the expression of HK2 gene, which encodes one of the key rate-limiting enzyme of glycolysis, was revealed, thus, the study of the mechanisms of its inhibition in CRC is of particular interest. To search for potential microRNAs, inhibiting the expression of HK2 in CRC, we have performed the analysis of data from “The Cancer Genome Atlas” (TCGA) and five microRNA–mRNA target interaction databases (TargetScan, DIANA microT, mirSVR (miRanda), PicTar, and miRTarBase) using original CrossHub software. Seven microRNAs containing binding site on mRNA HK2, which expression is negatively correlated with HK2 expression, were selected for further analysis. The expression levels of these microRNAs and mRNA HK2 were estimated by quantitative PCR on a set of CRC samples. It has been shown, that the expression of three microRNAs (miR-9-5p, -98-5p, and -199-5p) was increased and correlated negatively with mRNA level of HK2 gene. Thus, downregulation of HK2 gene may be caused by its negative regulation through microRNAs miR-9-5p, -98-5p, and -199-5p.

Keywords

colorectal cancer microRNA HK2 glycolysis qPCR 

Abbreviations

qPCR

quantitative PCR

CRC

colorectal cancer

microRNA

small noncoding RNA

miRNA-Seq

miRNA sequencing

RNA-Seq

RNA sequencing

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • A. V. Snezhkina
    • 1
  • G. S. Krasnov
    • 1
  • S. O. Zhikrivetskaya
    • 1
  • I. Y. Karpova
    • 1
  • M. S. Fedorova
    • 1
  • K. M. Nyushko
    • 2
  • M. M. Belyakov
    • 2
  • N. V. Gnuchev
    • 3
  • D. V. Sidorov
    • 2
  • B. Y. Alekseev
    • 2
  • N. V. Melnikova
    • 1
  • A. V. Kudryavtseva
    • 1
    • 2
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.National Medical Research Radiological CenterMinistry of Health of the Russian FederationMoscowRussia
  3. 3.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia

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