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Molecular Biology

, Volume 52, Issue 1, pp 23–29 | Cite as

Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases

  • A. G. Gabdulkhakov
  • O. S. Kostareva
  • I. A. Kolyadenko
  • A. O. Mikhaylina
  • L. I. Trubitsina
  • S. V. Tishchenko
Structural Functional Analysis of Biopolymers and Their Complexes
  • 26 Downloads

Abstract

Laccase belongs to the family of copper-containing oxidases. A study was made of the mechanism that sustains the incorporation of copper ions into the T2/T3 centers of recombinant two-domain laccase Streptomyces griseoflavus Ac-993. The occupancy of the T3 center by copper ions was found to increase with an increasing copper content in the culture medium and after dialysis of the protein preparation against a copper sulfate-containing buffer. The T2 center was filled only when overproducer strain cells were grown at a higher copper concentration in the medium. Two-domain laccases were assumed to possess a channel that serves to deliver copper ions to the T3 center during the formation of the three-dimensional laccase conformation and dialysis of the protein preparation. A narrower channel leads to the T2 center in two-domain laccases compared with three-domain ones, rendering the center less accessible for copper atoms. The incorporation of copper ions into the T2 center of two-domain laccases is likely to occur in the course of their biosynthesis or the formation of a functional trimer.

Keywords

two-domain laccases T2/T3 copper centers channels X-ray structures Streptomyces griseoflavus 

Abbreviations

2D

two-domain (laccase)

3D

three-domain (laccase)

SgSL

S. griseoflavus small laccase

SgSLmut

mutant SgSL

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • A. G. Gabdulkhakov
    • 1
  • O. S. Kostareva
    • 1
  • I. A. Kolyadenko
    • 1
  • A. O. Mikhaylina
    • 1
  • L. I. Trubitsina
    • 2
  • S. V. Tishchenko
    • 1
  1. 1.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow oblastRussia
  2. 2.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow oblastRussia

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