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Molecular Biology

, Volume 51, Issue 5, pp 748–758 | Cite as

Proteome of the human HaCaT keratinocytes: Identification of the oxidative stress proteins after sodium dodecyl sulpfate exposur

  • N. A. Petushkova
  • A. L. Rusanov
  • V. G. Zgoda
  • M. A. Pyatnitskiy
  • O. V. Larina
  • K. V. Nakhod
  • N. G. Luzgina
  • A. V. Lisitsa
Molecular Cell Biology

Abstract

Oxidative stress is a universal response of the skin cell damage of various origins. Sodium dodecyl sulfate (SDS, sodium lauryl sulfate) is an anionic surfactant commonly used as an emulsifying detergent in household cleaners. Sodium dodecyl sulfate is the reference compound for testing toxicity on cellular skin models. The effect of sodium dodecyl sulfate in sub toxic dose 25 μg/mL during 48 h on the protein profile of human keratinocytes HaCaT was studied by tandem mass spectrometry with electrospray ionization. In total, 1064 proteins were found in immortalized human keratinocytes HaCaT, of which about 80% were identified by two or more peptides. The change of the 217 proteins content was revealed, among them 39 according to Gene Ontology are associated with oxidative stress. It has been found that sodium dodecyl sulfate leads to a decrease in the number of proteins/peptides containing carboxymethylated and/or carboxyethylated lysine. We concluded about the promising of the cells redox-balance analysis at testing chemicals in the doses, which do not lead to a decrease in their viability. Possible involvement of sodium dodecyl sulfate in the development of cutaneous neoplasia is discussed.

Keywords

human keratinocytes HaCaT liquid chromatography tandem mass spectrometry sodium dodecyl sulphate oxidative stress 

Abbreviations

siRNA

small interfering RNA

VSR

viral suppressor of RNA silencing

RISC

RNA-induced silencing complex

PTGS

post-transcriptional gene silencing

TGS

transcriptional gene silencing

CLCuMuB

cotton leaf curl Multan betasatellite

DCL

Dicer-like (proteins)

dsRNA

double-stranded RNA

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Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  • N. A. Petushkova
    • 1
  • A. L. Rusanov
    • 2
  • V. G. Zgoda
    • 1
  • M. A. Pyatnitskiy
    • 1
  • O. V. Larina
    • 1
  • K. V. Nakhod
    • 1
  • N. G. Luzgina
    • 1
  • A. V. Lisitsa
    • 1
  1. 1.Institute of Biomedical ChemistryMoscowRussia
  2. 2.Research and Manufacturing Association “Perspectiva”NovosibirskRussia

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